The Giant Danio (<i>D. Aequipinnatus</i>) as A Model of Cardiac Remodeling and Regeneration

Lafontant, Pascal J.; Burns, Alan R.; Grivas, Jamie; Lesch, Mary Ann; Lala, Tanmoy Das; Reuter, Sean; Field, Loren J.; Frounfelter, Tyler

doi:10.1002/ar.21492

Cited by 50 publications

(73 citation statements)

References 85 publications

(124 reference statements)

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“…These procedures were previously described (Lafontant et al, 2012). Briefly, goldfish were anesthetized with 0.02% MS Tricaine for one to two minutes and placed ventral side up in a slit cut in a wet rectangular-shaped sponge block.…”

Section: Methodsmentioning

confidence: 99%

“…Semi-aquatic and aquatic vertebrates such as newts (Bader and Oberpriller, 1979; Witman et al, 2011; Piatkowski et al, 2013), axolotls (Flink, 2002; Vargas-Gonzalez et al, 2005), and teleosts such as the zebrafish (Poss et al, 2002) and giant danio (Lafontant et al, 2012) have been studied as models for cardiac injury, repair, and regeneration. Unlike mammals, significant adult heart regeneration has been documented in these species.…”

Section: Introductionmentioning

confidence: 99%

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Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

Grivas

Haag

Johnson

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The remarkable ability of the heart to regenerate has been demonstrated in the zebrafish and giant danio, two fish members of the cyprinid family. Here we use light and electron microscopy to examine the repair response in the heart of another cyprinid, the goldfish (Carassius auretus), following cautery injury to a small portion of its ventricular myocardium. We observed a robust inflammatory response in the first two weeks consisting primarily of infiltrating macrophages, heterophils, and melanomacrophages. These inflammatory cells were identified in the lumen of the spongy heart, within the site of the wound, and attached to endocardial cells adjacent to the site of injury. Marked accumulation of collagen fibers and increased connective tissue were also observed during the first and second week in a transition zone between healthy and injured myocardium as well as in adjacent sub-epicardial regions. The accumulation of collagen and connective tissue however did not persist. The presence of capillaries was also noted in the injured area during repair. The replacement of the cauterized region of the ventricle by myocardial tissue was achieved by 6 weeks. The presence of ethynyl deoxyuridine-positive cardiac myocytes and partially differentiated cardiac myocytes during repair suggest effective cardiac myocyte driven regeneration mechanisms also operate in the injured goldfish heart, and are similar to those observed in zebrafish and giant danio. Our data suggest the ability for cardiac regeneration may be widely conserved among cyprinids.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

Grivas

Haag

Johnson

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Although only a handful of model systems have been examined, it is now clear that at least certain urodele amphibians and teleost fish can regenerate portions of their hearts in a variety of severe injury models (Flink, 2002;Kikuchi et al, 2010;Lafontant et al, 2012;Poss et al, 2002;Wang et al, 2011). This process can replace most or all of the lost muscle locally in animals that are not concurrently undergoing cardiac growth, with local proliferative and gene expression responses.…”

Section: Animal Models For Injury-induced Adult Heart Regenerationmentioning

confidence: 99%

Building and re-building the heart by cardiomyocyte proliferation

2016

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The adult human heart does not regenerate significant amounts of lost tissue after injury. Rather than making new, functional muscle, human hearts are prone to scarring and hypertrophy, which can often lead to fatal arrhythmias and heart failure. The most-cited basis of this ineffective cardiac regeneration in mammals is the low proliferative capacity of adult cardiomyocytes. However, mammalian cardiomyocytes can avidly proliferate during fetal and neonatal development, and both adult zebrafish and neonatal mice can regenerate cardiac muscle after injury, suggesting that latent regenerative potential exists. Dissecting the cellular and molecular mechanisms that promote cardiomyocyte proliferation throughout life, deciphering why proliferative capacity normally dissipates in adult mammals, and deriving means to boost this capacity are primary goals in cardiovascular research. Here, we review our current understanding of how cardiomyocyte proliferation is regulated during heart development and regeneration.

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“…Although cardiac regeneration and repair have been investigated in other teleost models (Grivas et al, 2014;Ito et al, 2014;Lafontant et al, 2012), zebrafish arguably display the most robust and best characterized cardiac regenerative responses known to date among non-mammalian vertebrate models (Chablais et al, 2011;González-Rosa et al, 2011;Parente et al, 2013;Poss et al, 2002;Schnabel et al, 2011;Wang et al, 2011). This review will summarize recent advances in the field of regenerative medicine and discuss cellular and molecular mechanisms underlying the cardiac regenerative response in zebrafish.…”

Section: Introductionmentioning

confidence: 97%

Advances in understanding the mechanism of zebrafish heart regeneration

Kikuchi

2014

Stem Cell Research

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The adult mammalian heart was once believed to be a post-mitotic organ without any capacity for regeneration, but recent findings have challenged this dogma. A modified view assigns the mammalian heart a measurable capacity for regeneration throughout its lifetime, with the implication that endogenous regenerative capacity can be therapeutically stimulated in the injury setting. Although extremely limited in adult mammals, the natural capacity for organ regeneration is a conserved trait in certain vertebrates. Urodele amphibians and teleosts are well-known examples of such animals that can efficiently regenerate various organs including the heart as adults. By understanding how these animals regenerate a damaged heart, one might obtain valuable insights into how regeneration can be augmented in injured human hearts. Among the regenerative vertebrate models, the teleost zebrafish, Danio rerio, is arguably the best characterized with respect to cardiac regenerative responses. Knowledge is still limited, but a decade of research in this model has led to results that may help to understand how cardiac regeneration is naturally stimulated and maintained. This review surveys recent advances in the field and discusses current understanding of the endogenous mechanisms of cardiac regeneration in zebrafish.

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The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration

Cited by 50 publications

References 85 publications

Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

Cardiac repair and regenerative potential in the goldfish (Carassius auratus) heart

Building and re-building the heart by cardiomyocyte proliferation

Advances in understanding the mechanism of zebrafish heart regeneration

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