The ghr-miR164 and <i>GhNAC100</i> module participates in cotton plant defence against <i>Verticillium dahliae</i>

Hu, Guiying; Y, Lei; Wang, L; Liu, Jing; Tang, Yan; Zhang, Z; Chen, A; Qin, Peng; Yang, Zuoren; Wu, Jian

doi:10.1101/440826

Cited by 5 publications

(5 citation statements)

References 66 publications

(69 reference statements)

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“…The hlu-miR164b cleavage site within the transcript of hops NAC domain-containing protein was con rmed by 5' RLM-RACE analysis done by Mishra, et al [53]. Hu, et al [61] observed a signi cant decrease of ghr-miR164 in the response of cotton plants to infection with V. dahliae. Additionally, the researchers showed that ghr-miR164 directly cleaves the mRNA of GhNAC100, and silencing of ghr-miR164 leads to increased GhNAC100 expression, which in turn increases plant resistance to the fungus.…”

Section: Discussionmentioning

confidence: 92%

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

Kunej

Jakše

Radišek³

et al. 2021

Preprint

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Micro RNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biological processes, including plant response and resistance to fungal pathogens. Hops are grown for use in the brewing industry and recently also for pharmaceutical industry. Severe Verticillium wilt caused by the phytopathogenic fungus Verticillium nonalfalfae, is the main factor in yield loss in many crops, including hops (Humulus lupulus L.). In our study, we identified 56 known and 43 novel miRNAs and their expression patterns in the roots of susceptible and resistant hop cultivars after inoculation with V. nonalfalfae. In response to inoculation with V. nonalfalfae, we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. Differentially expressed miRNAs target 49 transcripts involved in protein localization and pigment synthesis in the susceptible cultivar, whereas they are involved in transcription factor regulation and hormone signalling in the resistant cultivar. The results of our study suggest that the susceptible and resistant hop cultivars respond differently to V. nonalfalfae inoculation at the miRNA level and that miRNAs may contribute to the successful defence of the resistant cultivar.

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Section: Discussionmentioning

confidence: 92%

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

Kunej

Jakše

Radišek³

et al. 2021

Preprint

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“…In our previous database, ghr-miR397 was identified to regulate 6 target genes namely Gh_A03G0417, Gh_D03G1128, Gh_A03G2084, Gh_D13G2373, Gh_A13G2215 and Gh_D13G2524, out of which, Gh_A03G0417 and Gh_D03G1128 showed remarkable upregulation expression under V. dahliae treatment ( Supplementary Figure 3 ) and located on the A and D subgenomes of G. hirsutum ( Hu et al, 2020b ). Therefore, two target genes were suffered to further research.…”

Section: Resultsmentioning

confidence: 99%

“…The GUS transient expression in the tobacco leaves, histochemical staining and activity quantified was performed as described by Hu et al (2020b) . In brief, the leaves were placed in pre-cooled 95% acetone on ice overnight.…”

Section: Methodsmentioning

confidence: 99%

A Cotton Lignin Biosynthesis Gene, GhLAC4, Fine-Tuned by ghr-miR397 Modulates Plant Resistance Against Verticillium dahliae

et al. 2021

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Plant lignin is a component of the cell wall, and plays important roles in the transport potential of water and mineral nutrition and plant defence against biotic stresses. Therefore, it is necessary to identify lignin biosynthesis-related genes and dissect their functions and underlying mechanisms. Here, we characterised a cotton LAC, GhLAC4, which participates in lignin biosynthesis and plant resistance against Verticillium dahliae. According to degradome sequencing and GUS reporter analysis, ghr-miR397 was identified to directedly cleave the GhLAC4 transcript through base complementary. GhLAC4 knockdown and ghr-miR397 overexpression significantly reduced basal lignin content compared to the control, whereas ghr-miR397 silencing significantly increased basal lignin levels. Based on staining patterns and GC/MS analysis, GhLAC4 acted in G-lignin biosynthesis. Under V. dahliae infection, we found that G-lignin content in ghr-miR397-knockdowned plants significantly increased, compared to these plants under the mock treatment, while G-lignin contents in GhLAC4-silenced plants and ghr-miR397-overexpressed plants treated with pathogen were comparable with these plants treated with mock, indicating that GhLAC4 participates in defence-induced G-lignin biosynthesis in the cell wall. Knockdown of ghr-miR397 in plants inoculated with V. dahliae promoted lignin accumulation and increased plant resistance. The overexpression of ghr-miR397 and knockdown of GhLAC4 reduced lignin content and showed higher susceptibility of plants to the fungal infection compared to the control. The extract-free stems of ghr-miR397-knockdowned plants lost significantly less weight when treated with commercial cellulase and V. dahliae secretion compared to the control, while the stems of ghr-miR397-overexpressed and GhLAC4-silenced plants showed significantly higher loss of weight. These results suggest that lignin protects plant cell walls from degradation mediated by cellulase or fungal secretions. In summary, the ghr-miR397-GhLAC4 module regulates both basal lignin and defence-induced lignin biosynthesis and increases plant resistance against infection by V. dahliae.

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“…In response to infection with V. nonalfalfae, the resistant cultivar showed signi cantly lower expression of hlu-miR164b, which targets transcripts of NAC domain-containing proteins. Chen, Peng, Yang and Wu [66] observed a signi cant decrease of ghr-miR164 in the response of cotton plants to infection with V. dahliae. Additionally, the researchers showed that ghr-miR164 directly cleaves the mRNA of GhNAC100, which in turn increased the resistance of plants to the fungus.…”

Section: Discussionmentioning

confidence: 96%

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

Kunej

Jakše

Radišek³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundMicro RNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biochemical and physiological processes, including plant response and resistance to fungal pathogens, by regulating the expression of numerous genes. Hops are grown for use in the brewing industry and have recently attracted the attention of the pharmaceutical industry. Severe Verticillium wilt disease caused by the phytopathogenic fungus Verticillium nonalfalfae is the main factor in yield loss in many crops, including hops (Humulus lupulus L.). ResultsIn our study, we identified miRNAs in hops and their expression patterns in the roots of susceptible and resistant hop cultivars in the early stages of infection with the fungus V. nonalfalfae. In total 56 known and 43 novel miRNAs were predicted. In response to infection with V. nonalfalfae, we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. Differentially expressed miRNAs target 49 transcripts and their gene ontology enrichment analysis showed that the susceptible cultivar responds by modulating the processes of protein localization and pigment synthesis, whereas the resistant cultivar responds by modulating transcription factors and hormone signalling.ConclusionThe results of our study suggest that the susceptible and the resistant cultivar respond differently at the miRNA level to infection with V. nonalfalfae and that miRNAs may contribute to the successful defence of the resistant cultivar.

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The ghr-miR164 and GhNAC100 module participates in cotton plant defence against Verticillium dahliae

Cited by 5 publications

References 66 publications

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

A Cotton Lignin Biosynthesis Gene, GhLAC4, Fine-Tuned by ghr-miR397 Modulates Plant Resistance Against Verticillium dahliae

Identification and Characterization of Verticillium Nonalfalfae-responsive Micrornas in the Roots of Resistant and Susceptible Hop Cultivars

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