The germline-specific expression of Foxl3a and its paralogous Foxl3b are associated with male gonadal differentiation in the Japanese eel, Anguilla japonica

Wu, Guan-Chung; Jeng, Shan-Ru; Pan, Yi-Tin; Li, Hau-Wen; Ku, Wei-Lun; Lin, Chien-Ju; Chang, Ching‐Fong

doi:10.1016/j.ygcen.2019.03.008

Cited by 13 publications

(7 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding was consistent with results from previous studies showing an absence of foxl3 in amphibians and placental mammals. 17 In the present study, investigation of a possible foxl3 paralogue in chickens revealed no results according to NCBI BLAST search (https://blast.ncbi.nlm.nih.gov/Blast.cgi) of current genome databases using chicken FOXL3-like amino acid sequence (our unpublished data). FOXL2 is related to ovarian development in mammals 14 and teleosts, 15 and in chickens, foxl2 mRNA is highly expressed in female gonads in eggs incubated for 5.7 days, with the timing of expression similar to that of the aromatase gene.…”

Section: Discussionmentioning

confidence: 56%

“…A recent report characterizing foxl3 in Japanese eel (Anguilla japonica) revealed the presence of two foxl3 genes (foxl3a and foxl3b) in this species. 17 In the present study, investigation of a possible foxl3 paralogue in chickens revealed no results according to NCBI BLAST search (https://blast.ncbi.nlm.nih.gov/Blast.cgi) of current genome databases using chicken FOXL3-like amino acid sequence (our unpublished data).…”

Section: Discussionmentioning

confidence: 56%

See 1 more Smart Citation

Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3‐like gene

Ichikawa

Ezaki

Furusawa

et al. 2019

Developmental Dynamics

View full text Add to dashboard Cite

Background Birds harbor specific sex determination and differentiation mechanisms. Although the molecular mechanisms associated with sex determination in somatic cells have been elucidated, those for germ cells remain unclear. Results Here, we characterized the chicken forkhead box L3 (foxl3)‐like gene as a sex‐determination factor in sexually indifferent medaka germline stem cells. The foxl3‐like gene was cloned by rapid amplification of cDNA ends, and the nucleotide sequence was analyzed. The deduced amino acid sequence was compared with FOXL3 sequences from other species, revealing low identity and similarity scores. Expression analysis of foxl3‐like mRNA during gonadogenesis showed female left‐gonad‐specific temporal expression in an egg incubated from 10 to 16 days, as well as low general expression in certain hatched female chicken organs. Moreover, the amino acid sequence deduced for the FOXL3‐like protein displayed low identity with medaka FOXL3, with the FOXL3‐like protein specifically localized in the oogonia, whereas medaka FOXL3 was found in sexually indifferent germline stem cells. Furthermore, the timing of expression differed between the foxl3‐like gene and that of medaka foxl3. Conclusions These results suggest that chicken FOXL3‐like protein and medaka FOXL3 differ in terms of their functions as female sex‐determination factors.

show abstract

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 56%

Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3‐like gene

Ichikawa

Ezaki

Furusawa

et al. 2019

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…Sex-related genes were sexually dimorphic expressed in the testicular and ovarian tissues. Some sex-related genes ( vasa , figla , sox3 , cyp19a1 , dmrt1 , sox9a , foxl3a , foxl3b , foxl2a ) were previously investigated during gonadal differentiation in Japanese eels [ 6 , 43 , 70 , 78 ]. We investigated the expression profiles of other sex-related genes according to the proposed model of gonadal sex development in Japanese eels ( Figure 12 ).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies on the Japanese eel reported the expression of some sex-specific genes ( vasa , cyp19a1 ; female: figla , sox3 ; male: dmrt1 , sox9a, foxl3a, foxl3b ) during gonadal differentiation and development [ 6 , 70 ]. However, the molecular regulators involved in sex determination and differentiation in eels need further investigation.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions

Lin

Jeng

Lei

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

The gonochoristic feature with environmental sex determination that occurs during the yellow stage in the eel provides an interesting model to investigate the mechanisms of gonadal development. We previously studied various sex-related genes during gonadal sex differentiation in Japanese eels. In the present study, the members of transforming growth factor beta (TGF-β) superfamily were investigated. Transcript levels of anti-Müllerian hormone, its receptor, gonadal soma-derived factor (amh, amhr2, and gsdf, respectively) measured by real-time polymerase chain reaction (qPCR) showed a strong sexual dimorphism. Transcripts were dominantly expressed in the testis, and their levels significantly increased with testicular differentiation. In contrast, the expressions of amh, amhr2, and gsdf transcripts were low in the ovary of E2-feminized female eels. In situ hybridization detected gsdf (but not amh) transcript signals in undifferentiated gonads. amh and gsdf signals were localized to Sertoli cells and had increased significantly with testicular differentiation. Weak gsdf and no amh signals were detected in early ovaries of E2-feminized female eels. Transcript levels of amh and gsdf (not amhr2) decreased during human chorionic gonadotropin (HCG)-induced spermatogenesis in males. This study suggests that amh, amhr2, and especially gsdf might be involved in the gene pathway regulating testicular differentiation of Japanese eels.

show abstract

“…Foxl3 is widely conserved in vertebrate genomes except for placental mammals ( Bertho et al, 2016 ). Although the role of Foxl3 in other vertebrate species remains largely unknown, its expression in germ cells has been reported in some teleost species including Nile tilapia ( Oreochromis niloticus ) and Japanese eel ( Anguilla Japonica ) ( Wu et al, 2019 ; Dai et al, 2021 ). Furthermore, a loss-of-function mutant of tilapia foxl3 leads to production of spermatogenic cells in XX ovaries ( Dai et al, 2021 ), suggesting a conserved role of foxl3 in germ cell feminization.…”

Section: Role Of Foxl3 In Germ Cell Sex Determination In Medakamentioning

confidence: 99%

Functional Modules in Gametogenesis

Kikuchi

Tanaka

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Gametogenesis, the production of eggs and sperm, is a fundamental process in sexually reproducing animals. Following gametogenesis commitment and sexual fate decision, germ cells undergo several developmental processes to halve their genomic size and acquire sex-specific characteristics of gametes, including cellular size, motility, and cell polarity. However, it remains unclear how different gametogenesis processes are initially integrated. With the advantages of the teleost fish medaka (Oryzias latipes), in which germline stem cells continuously produce eggs and sperm in mature gonads and a sexual switch gene in germ cells is identified, we found that distinct pathways initiate gametogenesis cooperatively after commitment to gametogenesis. This evokes the concept of functional modules, in which functionally interlocked genes are grouped to yield distinct gamete characteristics. The various combinations of modules may allow us to explain the evolution of diverse reproductive systems, such as parthenogenesis and hermaphroditism.

show abstract

The germline-specific expression of Foxl3a and its paralogous Foxl3b are associated with male gonadal differentiation in the Japanese eel, Anguilla japonica

Cited by 13 publications

References 51 publications

Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3‐like gene

Comparison of sex determination mechanism of germ cells between birds and fish: Cloning and expression analyses of chicken forkhead box L3‐like gene

Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions

Functional Modules in Gametogenesis

Contact Info

Product

Resources

About