The geographic origin of<i>Helicobacter pylori</i>isolated from Costa Rican patients

Molina-Castro, Silvia; Herrera, D. Martínez; Malespín-Bendaña, Wendy; Ramírez, Vanessa; Une, Clas

doi:10.4161/gmic.32148

Cited by 5 publications

(2 citation statements)

References 28 publications

(27 reference statements)

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“…While this result cannot be confirmed by the serum CagA antibody test, which does not distinguish between cagA allele types, this result is expected based on analysis of the cagA allele types of 33 Costa Rican H. pylori strains, all of which had Western type cagA gene [38]. Furthermore, an analysis of the geographical origin of 24 Costa Rican H. pylori strains characterized 21 as belonging to the hpEurope group and three as belonging to the hspWAfrica group [39], which would be expected to have cagA genes encoding an EPIYA-C motif. H. pylori strains of Amerindian origin have an EPIYA-DC motif with characteristics of both EPIYA-C and EPIYA-D motifs [40].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Detection and Genotyping of Helicobacter pylori from Stool using Droplet Digital PCR Reveals Variation in Bacterial Loads that Correlates with cagA Virulence Gene Carriage

et al. 2015

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Background Epidemiologic studies of the carcinogenic stomach bacterium Helicobacter pylori have been limited by the lack of non-invasive detection and genotyping methods. We developed a new stool-based method for detection, quantification, and partial genotyping of H. pylori using droplet digital PCR (ddPCR), which allows for increased sensitivity and absolute quantification by PCR partitioning. Materials and Methods Stool-based ddPCR assays for H. pylori 16S gene detection and cagA virulence gene typing were tested using a collection of 50 matched stool and serum samples from Costa Rican volunteers and 29 H. pylori stool antigen-tested stool samples collected at a U.S. hospital. Results The stool-based H. pylori 16S ddPCR assay had a sensitivity of 84% and 100% and a specificity of 100% and 71% compared to serology and stool antigen tests, respectively. The stool-based cagA genotyping assay detected cagA in 22 (88%) of 25 stools from CagA antibody-positive individuals and 4 (16%) of 25 stools from CagA antibody-negative individuals from Costa Rica. All 26 of these samples had a Western-type cagA allele. Presence of serum CagA antibodies was correlated with a significantly higher load of H. pylori in the stool. Conclusions The stool-based ddPCR assays are a sensitive, non-invasive method for detection, quantification, and partial genotyping of H. pylori. The quantitative nature of ddPCR-based H. pylori detection revealed significant variation in bacterial load among individuals that correlates with presence of the cagA virulence gene. These stool-based ddPCR assays will facilitate future population-based epidemiologic studies of this important human pathogen.

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Section: Discussionmentioning

confidence: 99%

Quantitative Detection and Genotyping of Helicobacter pylori from Stool using Droplet Digital PCR Reveals Variation in Bacterial Loads that Correlates with cagA Virulence Gene Carriage

et al. 2015

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“…Primers for PCR amplification and direct sequencing are shown in Additional file 4: Table S4. For construction of phylogenetic tree, 1293 MLST sequences of global strains were obtained from the previous studies: 229, 67, 113, 544, 92, 50, 128, 39, and 31 strains from hpAfrica1, hpAfrica2, hpNEAfrica, hpEurope, hpAsia2, hpSahul, hspEAsia, hspMaori, and hspAmerind, respectively (Additional file 5: Table S5) [1, 2, 11, 16, 18, 21, 2231, 46–53]. We used MLST sequences of our Dominican Republic strains and these global strains for the successive analyses.…”

Section: Methodsmentioning

confidence: 99%

Comparative study between Helicobacter pylori and host human genetics in the Dominican Republic

Ono

Cruz

Abreu³

et al. 2019

BMC Evol Biol

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BackgroundHelicobacter pylori, a bacterium that infects the human stomach, has high genetic diversity. Because its evolution is parallel to human, H. pylori is used as a tool to trace human migration. However, there are few studies about the relationship between phylogeography of H. pylori and its host human.MethodsWe examined both H. pylori DNA and the host mitochondrial DNA and Y-chromosome DNA obtained from a total 119 patients in the Dominican Republic, where human demography consists of various ancestries. DNA extracted from cultured H. pylori were analyzed by multi locus sequence typing. Mitochondrial DNA and Y-chromosome DNA were evaluated by haplogroup analyses.ResultsH. pylori strains were divided into 2 populations; 68 strains with African group (hpAfrica1) and 51 strains with European group (hpEurope). In Y-chromosomal haplogroup, European origin was dominant, whereas African origin was dominant both in H. pylori and in mtDNA haplogroup. These results supported the hypothesis that mother-to-child infection is predominant in H. pylori infection. The Amerindian type of mtDNA haplogroup was observed in 11.8% of the patients; however, Amerindian type (hspAmerind) of H. pylori was not observed. Although subpopulation type of most hpAfrica1 strains in Central America and South America were hybrid (hspWAfrica/hpEurope), most Dominican Republic hpAfrica1 strains were similar to those of African continent.ConclusionsGenetic features of H. pylori, mtDNA, and Y haplogroups reflect the history of colonial migration and slave trade in the Dominican Republic. Discrepancy between H. pylori and the host human genotypes support the hypothesis that adaptability of hspAmerind H. pylori strains are weaker than hpEurope strains. H. pylori strains in the Dominican Republic seem to contain larger proportion of African ancestry compared to other American continent strains.

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Population genetic structure of Helicobacter pylori strains from Portuguese‐speaking countries

2017

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The geographic origin ofHelicobacter pyloriisolated from Costa Rican patients

Cited by 5 publications

References 28 publications

Quantitative Detection and Genotyping of Helicobacter pylori from Stool using Droplet Digital PCR Reveals Variation in Bacterial Loads that Correlates with cagA Virulence Gene Carriage

Quantitative Detection and Genotyping of Helicobacter pylori from Stool using Droplet Digital PCR Reveals Variation in Bacterial Loads that Correlates with cagA Virulence Gene Carriage

Comparative study between Helicobacter pylori and host human genetics in the Dominican Republic

Population genetic structure of Helicobacter pylori strains from Portuguese‐speaking countries

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