The geographic distribution of Taura Syndrome Virus (TSV) in the Americas: determination by histopathology and in situ hybridization using TSV-specific cDNA probes

Hasson, Kenneth W.; Lightner, Donald V.; Mari, Jocelyne; Bonami, Jean Robert; Poulos, Bonnie T.; Mohney, Leone L.; Redman, Rita M.; Brock, James A.

doi:10.1016/s0044-8486(98)00429-3

Cited by 55 publications

(48 citation statements)

References 14 publications

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“…A commercially available in situ hybridization probe for TSV (Diagxotics Inc.) was used according to the manufacturer's instructions. The protocols have been outlined by Lightner (1996) and others , 1999a, Mari et al 1998.…”

Section: Methodsmentioning

confidence: 99%

“…ICENV (= TSV) is currently characterised as a picornavirus (Bonami et al 1997), and has been the focus of intense research , most of which has centred on development of sensitive detection techniques, its effects on different sizes or other species of penaeids , Lightner 1995, 1996, Lotz 1997, Overstreet et al 1997, Mari et al 1998, Nunan et al 1998, reports of geographic extension (Hasson et al 1999a), and progression of lesions under light microscopy (Hasson et al 1999b).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Periodic occurrence of epithelial viral necrosis outbreaks in Penaeus vannamei in Ecuador

Jiménez¹,

Barniol²,

Barniol³

et al. 2000

Dis. Aquat. Org.

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Epizootics of an infectious cuticular epithelial necrosis virus (ICENV) occurred in cultured Penaeus vannamei in Ecuadorian shrimp farms from 1994 to 1996. There were few reports of outbreaks during 1997, but in the second half of 1998 epizootics were again reported. Histopathological examination revealed extensive tissue changes and necrosis as described for infections by what others have called Taura syndrome virus (TSV). Infiltration of haemocytes in the cuticular epithelium was also one of the characteristics in the subacute and acute forms of this disease. Electron microscopy of affected tissues demonstrated the presence of a single type of virus particle in the cytoplasm of diseased shrimps from these outbreaks and it corresponded to the published descriptions for TSV. The epizootics of ICENV were periodic in occurrence, and data indicated that they might be related to the oceanographic and climatic variations reported in the eastern Pacific from 1994 to 1998. Using 3 mo rolling averages, a statistically significant negative correlation was found between prevalence of ICENV and temperature but not temperature change. By contrast, there was a statistically significant positive correlation between prevalence of ICENV and salinity change but not salinity value. Although these data do not establish causal relationships, they suggest that laboratory tests should be conducted to determine whether low temperature and upward changes in salinity can increase shrimp susceptibility to infection and mortality by ICENV. KEY WORDS: Infectious cuticular epithelial necrosis virus (ICENV) · Ultrastructure · Shrimp diseaseResale or republication not permitted without written consent of the publisher

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Periodic occurrence of epithelial viral necrosis outbreaks in Penaeus vannamei in Ecuador

Jiménez¹,

Barniol²,

Barniol³

et al. 2000

Dis. Aquat. Org.

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“…Following its initial discovery in 1992, TSV had spread to many shrimp farming regions, from South and Central America into North America by 1994 (Hasson et al 1999a) and to SE Asia by 1998 (Tu et al 1999, Yu & Song 2000.…”

Section: Introductionmentioning

confidence: 99%

Taura syndrome virus loads in Litopenaeus vannamei hemolymph following infection are related to differential mortality

Cao¹,

Wang²,

Breeland³

et al. 2010

Dis. Aquat. Org.

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“…Since the disease was first described in Ecuador in 1992, it has rapidly spread to many countries of the world (Jimenez 1992, 1999a, Tu et al 1999, Yu & Song 2002. The causative agent is Taura syndrome virus (TSV), which was placed in the family Dicistroviridae (Mayo 2002(Mayo , 2005.…”

Section: Introductionmentioning

confidence: 99%

Experimental infection of Penaeus monodon with Taura syndrome virus (TSV)

Srisuvan¹,

Tang²,

Lightner³

2005

Dis. Aquat. Org.

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Clinical signs and lesions of Taura syndrome virus (TSV) infection in Penaeus monodonhave not been documented although the virus has been detected in this shrimp species by reverse transcription polymerase chain reaction (RT-PCR). This study provides the first evidence of TSV infection in P. monodon by histological and in situ hybridization (ISH) analyses. We performed experimental bioassays with groups of P. monodon using inocula of P. monodon and Litopenaeus vannamei (Th04PmTSV and Th04LvTSV, respectively), which were collected from Thailand in 2004 and found to be positive for TSV by RT-PCR. Samples of shrimp for histological and ISH analyses were collected on Days 2, 14, and 28 post-inoculation. Mortality among TSV-inoculated P. monodon appeared on Day 3, with 2 out of 10 shrimp dying. Severe necrosis of cuticular epithelial cells and lymphoid organ spheroids, indicative of acute and chronic phase lesions of TSV infection, respectively, were detected in the samples. Sequence analyses of the capsid protein 2 (CP2) gene showed that Th04PmTSV and Th04LvTSV isolates were different; however, both belonged to a phylogenetic family of Asian TSV isolates. The results of this study demonstrated that both mortality and histological lesions are associated with TSV infection in P. monodon. KEY WORDS: Taura syndrome virus · TSV · Penaeus monodon · TSV infectionResale or republication not permitted without written consent of the publisher

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The geographic distribution of Taura Syndrome Virus (TSV) in the Americas: determination by histopathology and in situ hybridization using TSV-specific cDNA probes

Cited by 55 publications

References 14 publications

Periodic occurrence of epithelial viral necrosis outbreaks in Penaeus vannamei in Ecuador

Periodic occurrence of epithelial viral necrosis outbreaks in Penaeus vannamei in Ecuador

Taura syndrome virus loads in Litopenaeus vannamei hemolymph following infection are related to differential mortality

Experimental infection of Penaeus monodon with Taura syndrome virus (TSV)

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