The genus Simplicillium

Abstract

Simplicillium species have a wide host range and an extensive distribution. Some species are associated with rusts, as well as other plant pathogenic fungi and play an important role in biological control. In this study, two specimens of Simplicillium were collected from Chiang Mai Province, Thailand. Simplicillium formicae sp. nov. was isolated from an infected ant and S. lanosoniveum from Ophiocordyceps unilateralis which is a new host record. Species were initially identified using ITS gene sequences and confirmed using morphology coupled with phylogenetic analyses of a combined nrLSU, nrSSU, TEF and RPB1 dataset. Simplicillium formicae differs from other species in the genus by the presence of flask-shaped synnemata and phialides with intercalary nodes. Simplicillium lanosoniveum resembles other collections of the species by its completely solitary, tapering phialides and globose to ellipsoidal conidia which adhere in a slimly head. A key to species of Simplicillium is also provided.

Taxonomic and phylogenetic characterizations reveal four new species of Simplicillium (Cordycipitaceae, Hypocreales) from Guizhou, China

“…In the present study, S. chinense , S. coffeanum and S. filiform were clustered into an independent clade (Fig. 2 ), and supported by Crous et al 7 , Chen et al 20 and Wei et al 28 . Thus, L. filiform is proposed as a new combination.…”

“…Lasergene software (version 6.0, DNASTAR) was applied for the assembling and editing of DNA sequence in this study. The ITS , LSU , SSU , RPB1 and TEF sequences were downloaded from GenBank, based on Nonaka et al 5 , Zhang et al 8 , Gomes et al 6 , Crous et al 7 , Mongkolsamrit et al 27 , Chen et al 20 , Wei et al 28 , Kondo et al 29 and others selected on the basis of BLAST algorithm-based searches in GenBank (Table 2 ). The Multiple datasets of ITS , LSU , SSU , RPB1 and TEF were aligned and edited by MAFFT v7.037b 30 and MEGA6 31 .…”

Simplicilones A and B Isolated from the Endophytic Fungus Simplicillium subtropicum SPC3

“…The genus Simplicillium belongs to the Ascomycota and they have a wide host range and an extensive distribution. Since some of them play an important role in biological control [15], we investigated the strain SPC3 for its secondary metabolite production. We herein report the isolation and structural elucidation of simplicilones A and B, two secondary metabolites produced by S. subtropicum isolated from the Cameroonian medicinal plant D. staudtii (Annonaceae).…”

Identification of a Hyperparasitic Simplicillium obclavatum Strain Affecting the Infection Dynamics of Puccinia striiformis f. sp. tritici on Wheat

“…and Simplicillium spp. ( Zare and Gams, 2008 ; Wei et al, 2019 ). However, these morphological characteristics are considerably variable in different culture conditions and environments.…”