The genus Leuconostoc

In vitro ability of lactic acid bacteria to inhibit mastitis-causing pathogens

“…Gram-positive, catalase negative, oxidase negative (Bactident s Oxidase; Merck, Darmstadt, Germany), fermentative (OF basal medium with addition of D (þ )-glucose monohydrate; Merck, Darmstadt, Germany), H 2 S and indole negative, immotile (SIM agar; Merck, Darmstadt, Germany) rods or cocci were considered as presumptive LAB and were stored in MRS broth containing 20% glycerol at À 80°C. Additionally, esculin hydrolysis (esculin blood agar; Oxoid, Wesel, Germany) and growth in 6.5% NaCl Standard-I-Nutrient-Broth (Merck, Darmstadt, Germany) or at 45°C in MRS broth were tested (Björkroth et al, 2014;Franz et al, 2014;Pot et al, 2014;Teuber and Geis, 2006). For all subsequent assays, LAB were cultured anaerobically in MRS broth at 37°C for 24 h and subcultivated under the same conditions.…”

Analysis of rpoB polymorphism and PCR-based approaches for the identification of Leuconostoc mesenteroides at the species and subspecies level

“…The genomic DNA was isolated with the GeneElute Bacterial Genomic DNA Kit (Sigma-Aldrich Srl, Milan, Italy) and quantified with a Nan-oDrop®1000c spectrophotometer (Thermo Scientific, Wilmington, DE). Forward and reverse rpoB gene primers (Supplementary Table 2) were designed with Primer Express v.3.0 (Applied Biosystems, Concord, On Lactobacillus brevis (Chun et al, 2017b) 575 (FS); B29, F02 (SD); TO62 (RM) Leuconostoc carnosum (Campedelli et al, 2015) 102, 1249 (FS) Leuconostoc citreum (Campedelli et al, 2015) D27, V2 (Endo and Okada, 2008) 1034 (FS); M65 (RM); W2, W4, W5, Z02, Z11 (SD) Weissella cibaria (Dicks et al, 1995) E66, D4, O3, MTE22L, MTE5L (SD); DSM15878 (chili) Weissella confusa (Björkroth et al, 2014) DSM20196 (sugar cane) Weissella hellenica (Björkroth et al, 2014) DSM7378 (FS) Weissella minor (Björkroth et al, 2014) DSM20014 (milking machine slime) Weissella viridescens (Björkroth et al, 2014) DSM20410 (cured meat products) where not specified, the strains belong to the culture collection of the Laboratory of Industrial Microbiology, Università degli Studi della Basilicata (Potenza, Italy). Bold: strains used for the phylogenetic analysis of rpoB gene (see Fig.…”

Antimicrobial Efficacy of Leuconostoc spp. Isolated from Indian Meat against Escherichia coli and Listeria monocytogenes in Spinach Leaves

“…has been isolated from multiple and different habitats and they play an integral role in the fermentation of food. Leuconostocs are generally considered as GRAS (generally regarded as safe) organisms (Bjorkroth et al, 2014) as they are associated with food fermentation. However, some species of Leuconostoc have been associated with opportunistic infection (Kumudhan et al, 2004) in immune compromised patients and this microorganism can cause spoilage in some types of food matrices (de Paula et al, 2015).…”