The genus <i>Pseudo-nitzschia</i> (Bacillariophyceae) in New Zealand: analysis of the last decade's monitoring data

Rhodes, Lesley; Jiang, Weimin; Knight, Ben; Adamson, Janet; Smith, Kirsty F.; Langi, V.A.; Edgar, Mandy

doi:10.1080/00288330.2013.803489

Cited by 21 publications

(38 citation statements)

References 22 publications

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“…Different areas of the Southern Ocean show great variability in plankton diversity (Almandoz et al, 2008;Hasle, 2002;Trainer et al, 2012). Several Pseudo-nitzschia species have been reported to contribute to local diatom communities (Almandoz et al, 2008;Hegseth and Von Quillfeldt, 2002), however, only P. turgidula showed low toxicity (Rhodes et al, 2013). Potential sources of dDA in the Southern Ocean are thus scarce, which matches low dDA concentrations determined in our study.…”

Section: Spatial Distribution Of Dissolved Domoic Acid In the Easternsupporting

confidence: 82%

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

et al. 2019

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The widespread diatom Pseudo-nitzschia can produce domoic acid (DA). DA is a compound with well described neurotoxic effects on vertebrates including humans known as amnesic shellfish poisoning (ASP) syndrome. It has also been suggested to serve as an organic ligand that binds to iron and copper. By binding these trace elements, DA may increase their solubility and bioavailability. In order to serve this function, DA has to be excreted and reabsorbed by the cells. Only few records of dissolved domoic acid (dDA) concentrations in the ocean exist. To accomplish quantification by ultra performance liquid chromatography (UPLC), samples have to be pre-concentrated and desalted using solid-phase extraction, a procedure commonly applied for dissolved organic matter. Our major goals were to quantify dDA in a basin-wide assessment in the East Atlantic Ocean, to determine extraction efficiencies for complexed and uncomplexed dDA, and to assess whether domoic acid is represented by its molecular formula in direct-infusion high resolution mass spectrometry. Our results showed that dDA was extracted almost quantitatively and occurred ubiquitously in the ocean surface but also in deeper (and older) water, indicating surprisingly high stability in seawater. The maximum concentration measured was 173 pmol L −1 and the average molar dDA carbon yield was 7.7 ppm. Both carbon yield and dDA concentration decreased with increasing water depth. Providing quantification of dDA in the water column, we seek to improve our understanding of toxic bloom dynamics and the mechanistic understanding of DA production.

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Section: Spatial Distribution Of Dissolved Domoic Acid In the Easternsupporting

confidence: 82%

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

et al. 2019

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“…These concentrations are similar to those reported in the review by Trainer et al (2012;up to 30 fg Á cell À1 ) and in Lema et al (2017;up to 55 fg Á cell À1 ), though in the lower range of concentrations. For P. pungens, in contrast with studies reporting nontoxigenic strains (Villac et al 1993 and references therein), each of our strains produced DA, even if the measured cDA concentrations (0.2-50 fg Á cell À1 ) were also in the lower range compared with previous studies , Baugh et al 2006, Calu et al 2009, Rhodes et al 2013, Lema et al 2017. Pseudo-nitzschia pungens and P. fraudulenta also consistently produced dissolved domoic acid.…”

Section: Discussioncontrasting

confidence: 68%

Differential Influence of Life Cycle on Growth and Toxin Production of three Pseudo‐nitzschia Species (Bacillariophyceae)

Sauvey

Claquin

Roy

et al. 2019

Journal of Phycology

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We used a multistrain approach to study the intra‐ and interspecific variability of the growth rates of three Pseudo‐nitzschia species – P. australis, P. fraudulenta, and P. pungens – and of their domoic acid (DA) production. We carried out mating and batch experiments to investigate the respective effects of strain age and cell size, and thus the influence of their life cycle on the physiology of these species. The cell size – life cycle relationship was characteristic of each species. The influence of age and cell size on the intraspecific variability of growth rates suggests that these characteristics should be considered cautiously for the strains used in physiological studies on Pseudo‐nitzschia species. The results from all three species do not support the hypothesis of a decrease in DA production with time since isolation from natural populations. In P. australis, the cellular DA content was rather a function of cell size. More particularly, cells at the gametangia stage of their life cycle contained up to six times more DA than smaller or larger cells incapable of sexual reproduction. These findings reveal a link between P. australis life cycle and cell toxicity. This suggest that life cycle dynamics in Pseudo‐nitzschia natural populations may influence bloom toxicity.

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“…The strain IMP-BG 440 tested was able to produce the toxin in culture with a concentration between 0.004 and 0.010 pg cell −1 , which is comparable to those reported by Pistocchi, et al [49] (0.003 pg cell −1 ) in a strain obtained from the Adriatic Sea that was cultured under similar conditions (16-18 • C; 60-100 µmol photons m −2 s −1 ). These concentrations were lower than the values reported in Australian strains by Ajani, et al [63] (1-11 pg cell −1 ), Rhodes, et al [64] (1.5 pg cell −1 ) and in Italian strains registered by Amato, et al [65] (0.28 pg cell −1 ), Orsini, et al [47] (0.69 pg cell −1 ) and Sarno [48] (0.65 pg cell −1 ). The Humboldt Current system (HCS) is considered one of the most productive fishery regions in the world oceans [33,34,66,67].…”

Section: Discussioncontrasting

confidence: 65%

First Report of Domoic Acid Production from Pseudo-nitzschia multistriata in Paracas Bay (Peru)

Tenorio

Álvarez

Quijano-Scheggia

et al. 2021

Toxins

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The Peruvian sea is one of the most productive ecosystems in the world. Phytoplankton production provides food for fish, mammals, mollusks and birds. This trophic network is affected by the presence of toxic phytoplankton species. In July 2017, samples of phytoplankton were obtained from Paracas Bay, an important zone for scallop (Argopecten purpuratus) aquaculture in Peru. Morphological analysis revealed the presence of the genus Pseudo-nitzschia, which was isolated and cultivated in laboratory conditions. Subsequently, the monoclonal cultures were observed by scanning electron microscopy (SEM), and identified as P. multistriata, based on both the morphological characteristics, and internal transcribed spacers region (ITS2) sequence phylogenetic analysis. Toxin analysis using liquid chromatography (LC) with high-resolution mass spectrometry (HRMS) revealed the presence of domoic acid (DA) with an estimated amount of 0.004 to 0.010 pg cell−1. This is the first report of DA from the coastal waters of Peru and its detection in P. multistriata indicates that it is a potential risk. Based on our results, routine monitoring of this genus should be considered in order to ensure public health.

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The genus Pseudo-nitzschia (Bacillariophyceae) in New Zealand: analysis of the last decade's monitoring data

Cited by 21 publications

References 22 publications

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

Quantification, extractability and stability of dissolved domoic acid within marine dissolved organic matter

Differential Influence of Life Cycle on Growth and Toxin Production of three Pseudo‐nitzschia Species (Bacillariophyceae)

First Report of Domoic Acid Production from Pseudo-nitzschia multistriata in Paracas Bay (Peru)

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