The Genus Artemisia and its Allies: Phylogeny of the Subtribe Artemisiinae (Asteraceae, Anthemideae) Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)

Abstract

Sequences of the internal transcribed spacers (ITS1 and ITS2) of nuclear ribosomal DNA were analysed for 44 Artemisia species (46 populations) representing all the five classical subgenera and the geographical range of the genus, 11 species from 10 genera closely related to Artemisia, and six outgroup species from five other genera of the Anthemideae. The results definitely support the monophyly of the genus Artemisia in its broadest sense (including some Hippolytia, Kaschgaria). Our results, correlated to other data, such as pollen morphology, allow us to conclude that the subtribe Artemisiinae as currently defined is a very heterogeneous group. Affinities of the largest genus of the subtribe and tribe, Artemisia, and of other genera of the subtribe to some genera from other subtribes of the Anthemideae strongly suggest that subtribe Artemisiinae needs a deep revision and redefinition. Phylogenetic utility of region trnL-F of the plastid DNA in the genus Artemisia and allies was also evaluated; sequences of the trnL-F region in Artemisia do not provide phylogenetic information.

Molecular Authentication of 21 Korean Artemisia Species (Compositae) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Based on trnL-F Region of Chloroplast DNA

“…Together, these results suggest that the trnL-F sequence variations have a relatively low mutation frequency and therefore are limited in their utility in evaluating phylogenic relationship of Artemisia species collected in Korea. 25) This may be because cpDNA has generally uniparental inheritance, which does not undergo genetic recombination. 26,27) From the some SNPs in the trnL-F sequences (Fig.…”

New or rare data on chromosome numbers and karyomorphology of some taxa in the subgenusSeriphidium(Bess.) Rouy. (Artemisia, Asteraceae) in Turkey

“…The genus comprises around 600 taxa at the specific or subspecific level, according to various authors (Ling 1991a(Ling , 1991b(Ling , 1995a(Ling , 1995bBremer and Humphries 1993;Vallès and McArthur 2001;Kadereit and Jeffrey 2007;Vallès et al 2011). Because of the high number of taxa, numerous taxonomists are trying to solve the problem of its classification and phylogeny but its natural classification still has not been achieved (McArthur et al 1981;Torrell et al 1999;Vallès et al 2003;. Moreover, Artemisia is a taxonomically complex genus because some species have different morphological forms and others closely resemble each other.…”

“…Less., Dracunculus (Besser) Rydb., Seriphidium Besser and Tridentatae (Rydb.) McArthur] but subgeneric classification is subject to rearrangements in the light of recent molecular studies (Torrell et al 1999;Vallès et al 2003). Although these five large groups are usually recognized within the genus, treated as sections or subgenera depending on the authors consulted (Shishkin and Bobrov 1995;Vallès et al 2003; and references therein), a comprehensive infrageneric classification has…”

Molecular cytogenetic characterization of some representatives of the subgenera <i>Artemisia</i> and <i>Absinthium</i> (genus <i>Artemisia</i>, Asteraceae)

“…Nevertheless, this classification does not accurately represent natural groups. Molecular phylogenetic studies, based on the analysis of chloroplast (cpDNA) and nuclear ribosomal (nrDNA) sequences, have helped elucidating the systematic relationships within Artemisia (KORNKVEN et al, 1998;TORRELL et al, 1999;WATSON et al, 2002;VALLÈS et al, 2003;SANZ et al, 2008), although important questions still remain unresolved.…”