The genus Daphne: A review of its traditional uses, phytochemistry and pharmacology

Moshiashvili, G; Tabatadze, Nino; Mshvildadze, Vakhtang

doi:10.1016/j.fitote.2020.104540

Cited by 55 publications

(31 citation statements)

References 148 publications

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“…Some miRNAs show a suppressive effect on viral replication but are also toxic to the host when they are highly expressed. In comparison, other naturally occurring products capable of suppressing EV71 activity may have stronger side effects in patients such as the famously toxic plant Daphne Genkwa that may cause circulatory arrest and internal bleeding [86] or Rheum palmatum that induces gastroenteritis and diarrhea [87,88]. Honeysuckle is a traditional herb that has long been used to treat diverse diseases including flu-like symptoms in China and is not known to induce any side effects.…”

Section: Discussionmentioning

confidence: 99%

Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Lee

Chang

Yeh

et al. 2021

Viruses

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Honeysuckle (Lonicera japonica Thunb) is a traditional Chinese medicine (TCM) with an antipathogenic activity. MicroRNAs (miRNAs) are small non-coding RNA molecules that are ubiquitously expressed in cells. Endogenous miRNA may function as an innate response to block pathogen invasion. The miRNA expression profiles of both mice and humans after the ingestion of honeysuckle were obtained. Fifteen overexpressed miRNAs overlapped and were predicted to be capable of targeting three viruses: dengue virus (DENV), enterovirus 71 (EV71) and SARS-CoV-2. Among them, let-7a was examined to be capable of targeting the EV71 RNA genome by reporter assay and Western blotting. Moreover, honeysuckle-induced let-7a suppression of EV71 RNA and protein expression as well as viral replication were investigated both in vitro and in vivo. We demonstrated that let-7a targeted EV71 at the predicted sequences using luciferase reporter plasmids as well as two infectious replicons (pMP4-y-5 and pTOPO-4643). The suppression of EV71 replication and viral load was demonstrated in two cell lines by luciferase activity, RT-PCR, real-time PCR, Western blotting and plaque assay. Furthermore, EV71-infected suckling mice fed honeysuckle extract or inoculated with let-7a showed decreased clinical scores and a prolonged survival time accompanied with decreased viral RNA, protein expression and virus titer. The ingestion of honeysuckle attenuates EV71 replication and related pathogenesis partially through the upregulation of let-7a expression both in vitro and in vivo. Our previous report and the current findings imply that both honeysuckle and upregulated let-7a can execute a suppressive function against the replication of DENV and EV71. Taken together, this evidence indicates that honeysuckle can induce the expression of let-7a and that this miRNA as well as 11 other miRNAs have great potential to prevent and suppress EV71 replication.

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Section: Discussionmentioning

confidence: 99%

Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Lee

Chang

Yeh

et al. 2021

Viruses

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“…Daphne L. is one of the most diverse genera in the Thymelaeaceae family, with over 90 described species distributed in Asia, parts of North Africa, and Europe. Several species of this genus are used in traditional medicine for their antimicrobial, anticancer, anti-inflammatory, antitussive, and anti-rheumatic properties [1]. Terpenoids, phenols coumarins, lignans, and flavonoids are the most important classes of compounds identified in the genus Daphne [1].…”

Section: Introductionmentioning

confidence: 99%

Extracts of Different Polarity of Daphne laureola L. as Valuable Source of Antioxidant and Neuroprotective Compounds

Tundis

Cappello

Bonesi

et al. 2020

The 1st International E-Conference on Antioxidants in Health and Disease

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In this study, Daphne laureola L., a European–Mediterranean species, was investigated for its antioxidant properties using different in vitro bioassays, namely 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Activity Power (FRAP), and β-carotene bleaching assays. The acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity using Ellman’s method was also examined. The aerial parts of D. laureola were subjected to exhaustive and subsequent macerations with solvents at different polarities, such as methanol, dichloromethane, and n-hexane. Dichloromethane extract was the most promising in DPPH and FRAP tests with IC50 values of 32.2 μg/mL and 71.5 μM Fe(II)/g, respectively. Methanol extract showed the most promising inhibition of lipid peroxidation evaluated by β-carotene bleaching test with an IC50 value of 6.6 μg/mL after 30 min of incubation. This extract was the most active against AChE (IC50 value of 56.9 μg/mL). An interesting result was obtained against BChE by the n-hexane extract, with an IC50 value of 49.7 μg/mL. In conclusion, the results suggest that D. laureola may provide a substantial source of phytochemicals, which act as natural antioxidants and acetylcholinesterase inhibitors, and may be beneficial in the treatment of Alzheimer disease.

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“…In recent years, more than 250 chemical constituents have been identified from the genus Daphne. The most important classes of compounds obtained from this genus include coumarins, flavonoids, lignans, terpenoids, and several other less common groups (Xu et al, 2011;Moshiashvili et al, 2020) Coumarins are among the most common compounds in the Daphne genus and major toxic components. Daphne genus-related potential risks include hepatotoxicity, nephrotoxicity, burning in the pharynx, gastrointestinal discomfort (nausea, vomiting, burning of stomach, gastroenteritis, and diarrhea), internal bleeding, muscle spasms and paralysis, and skin irritation (redness, swelling, and blusters).…”

Section: Discussionmentioning

confidence: 99%

“…Daphne genus-related potential risks include hepatotoxicity, nephrotoxicity, burning in the pharynx, gastrointestinal discomfort (nausea, vomiting, burning of stomach, gastroenteritis, and diarrhea), internal bleeding, muscle spasms and paralysis, and skin irritation (redness, swelling, and blusters). In severe cases, circulatory arrest and coma may be observed " (Goel et al, 2007;Xu et al, 2011;Moshiashvili et al, 2020;Wink, 2009;Stefanachi et al, 2018)." Therefore, we should pay more attention to the safety of the Cortex Daphnes patch.…”

Section: Discussionmentioning

confidence: 99%

Clinical Efficacy of Cortex Daphnes (Zushima) Patch in Patients With Symptomatic Knee Osteoarthritis: A Multicenter Non-Inferiority Randomized Controlled Clinical Trial

Jiao

Zhang

et al. 2021

Front. Pharmacol.

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Background: Osteoarthritis (OA) is imposing substantial burdens on individuals and society with the aging population. Cortex Daphnes patch is widely used for symptomatic knee OA in China with a satisfying clinical efficacy; however, there is scant clinical evidence supporting its use. To evaluate its efficacy, we conducted a multicenter, non-inferiority, randomized, parallel-group study comparing Cortex Daphnes patch with topical nonsteroidal anti-inflammatory drugs in patients with knee OA (NCT02770950).Methods: A total of 264 symptomatic knee OA patients were treated with Cortex Daphnes or indomethacin cataplasms applied to affected sites once daily for 2 weeks. The primary outcome was improvement in knee pain on walking as assessed using a visual analog scale (VAS). The non-inferiority margin based on the full analysis population was set as –5 mm on the pain VAS. The secondary outcomes were changes of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score, WOMAC scores for pain, function and stiffness, the 36-item Short Form Health Survey (SF-36), and global assessment of knees by the patients. Responder rates for pain VAS, WOMAC total score, and WOMAC pain were also included in the secondary outcomes.Results: The Cortex Daphnes patch was non-inferior to indomethacin cataplasms for the primary outcome with a group difference (Cortex Daphnes patch–indomethacin cataplasm) of 2.1 mm (95% confidence interval: 2.1–6.4); similar results were found in the per-protocol population. For all other outcomes, no significant differences were found in the full analysis set or in the per-protocol analysis set, except the responder rates for WOMAC pain was higher in the Cortex Daphnes patch group than in the indomethacin cataplasm group (78.4 vs. 64.7%, p = 0.022) in the per-protocol analysis set. Overall, 28.8% patients in the Cortex Daphnes patch group and 9.8% in the indomethacin cataplasm group reported treatment-related adverse events, the vast majority of which were mild-to-moderate skin irritation, resulting in only 3.8 and 0.8% of patients dropping out, respectively.Conclusion: The Cortex Daphnes patch, which provides satisfactory analgesic efficacy and enhances the physical function of the knee, as well as improving quality of life, may be a promising alternative to knee OA.

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The genus Daphne: A review of its traditional uses, phytochemistry and pharmacology

Cited by 55 publications

References 148 publications

Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2

Extracts of Different Polarity of Daphne laureola L. as Valuable Source of Antioxidant and Neuroprotective Compounds

Clinical Efficacy of Cortex Daphnes (Zushima) Patch in Patients With Symptomatic Knee Osteoarthritis: A Multicenter Non-Inferiority Randomized Controlled Clinical Trial

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