The Genotype of Aujeszky's Disease Viruses Isolated in Argentina.

Echeverría, María Gabriela; Norimine, Junzo; Galosi, Cecília Mônica; Oliva, Graciela A.; Etcheverrigaray, María Elisa; Nosetto, Edgardo Omar; Tohya, Yukinobu; Mikami, Takeshi

doi:10.1292/jvms.56.985

Cited by 11 publications

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“…Subtyping of the Argentinean field isolates of SuHV-1 (Serena et al, 2010) has clearly shown that all strains are genotype I, the same as that occurred in Central Europe (Herrmann et al, 1984), Northern Ireland (Todd and Mc Ferran, 1985), the United States (Ben-Porat et al, 1984;Pirtle et al, 1984), and New Zealand (Tisdall et al, 1988). Although only one strain isolated in Argentina and previously classified as genotype II has been isolated from animals imported from Holland (Echeverría et al, 1994), no new isolates belonging to genotype II have been reported since 1981. Previous reports mention that genotype II is common in Holland (Gielkens et al, 1985).…”

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confidence: 89%

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Serena¹,

Metz²,

Lozada³

et al. 2018

Open Vet J.

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Since Aujeszky`s disease (pseudorabies), which is caused by Suid herpesvirus type 1 (SuHV-1), was first notified in Argentina in 1978, many SuHV-1 strains have been isolated from swine. However, this disease can affect other vertebrates, such as dogs (secondary hosts), and lead to fatal neurological disease. The objective of the current work is to report the first isolation and molecular characterization of SuHV-1 from a dead domestic dog from Santa Fe Province (Argentina), which had had nervous signs compatible with pseudorabies. Samples of brain and trigeminal ganglia from this dog were obtained and fixed in formol for histopathology, and virology studies were conducted after cell disruption. Supernatants of both samples were inoculated onto RK13 cells and, after 72 h, DNA was extracted with phenol-chloroform. Purified DNA was cut with a restriction enzyme and subjected to agarose gel and an aliquot was used to amplify the gD and gC genes by PCR. The gC sequence was compared with other public sequences. The strain isolated from the dog was similar to other Argentinean swine strains.

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confidence: 89%

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Serena¹,

Metz²,

Lozada³

et al. 2018

Open Vet J.

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“…However, this hypothesis remains to be tested using molecular biology techniques to compare both isolates (Echeverria et al, 1994;Nosetto et al, 1997). In the case of farms 2 and 3, an outside source of transmission, such as newly introduced breeding stock seems the most likely source of infection.…”

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confidence: 99%

Outbreaks of Aujeszky’s Disease in Pigs from Panama

Obaldía

2005

Trop Anim Health Prod

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“…DNA was isolated from the Continuous Porcine Kidney cell line [14] (CPK) infected with the CL-15 SHV-1 strain [15] at a multiplicity of infection between 1 and 5, as follows. CPK cells were grown in 75 cm 2 plastic flasks, inoculated with 1 ml of supernatant from positive cell cultures and, after extensive cytopathic effect was observed, cell pellets were washed and suspended in TEN buffer (100 mM Tris-HCl; pH7.5, 12.5 mM EDTA; pH 8.0, 150 mM NaCl, and 1% SDS).…”

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confidence: 99%

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

Serena

Geisler

Metz

et al. 2013

Protein Expression and Purification

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Suid Herpesvirus 1 (SHV-1) is the etiological agent of Aujeszky’s disease (AD), which affects swine herds worldwide and causes substantial economic losses due to animal mortality and lost productivity. In order to eradicate SHV-1, vaccination programs using viruses lacking the gene encoding glycoprotein E (gE) are ongoing in several countries. These eradication programs have generated a currently unmet demand for affordable and sensitive tests that can detect SHV-1 infection, yet distinguish between infected and vaccinated pigs. To meet this demand, we used the baculovirus-insect cell system to produce immunologically authentic full-length recombinant gE protein for use in a serum ELISA assay. As previous efforts to clone the gE gene had failed due to its extremely high GC-content (75% average), we used betaine as a PCR enhancer to facilitate amplification of the entire gE gene from the Argentinian CL15 strain of SHV-1. The cloned gE gene was expressed at high levels in recombinant baculovirus-infected insect cells and reacted strongly with sera from SHV-1 infected pigs. We used the recombinant gE protein to develop a local indirect ELISA test with sensitivity and specificity comparable to currently available commercial tests. Thus, recombinant gE produced in baculovirus-infected insect cells is a viable source of antigen for the detection of SHV-1 in ELISA tests. We also provide evidence supporting a potential application of this recombinant form of gE as a SHV-1 subunit vaccine.

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The Genotype of Aujeszky's Disease Viruses Isolated in Argentina.

Cited by 11 publications

References 0 publications

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

First isolation and molecular characterization of Suid herpesvirus type 1 from a domestic dog in Argentina

Outbreaks of Aujeszky’s Disease in Pigs from Panama

Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

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