The genotoxic effects of logran on<i>Hordeum vulgare</i>L. and<i>Triticum aestivum</i>L.

“…The confirmation of macroscopic effects by microscopic events is in accordance with statement of other authors (Aybeke et al, 2008;Kaymak and Muranli, 2006) that T. aestivum is a suitable test plant for toxicity testing.…”

Section: Allium Cepa-testsupporting

confidence: 89%

Synthesis and Cytostatic Activity of 4-bromo-5-ethyl-2-(ethylamino)- 5-methyl-5H-1,2-oxaphosphole 2-oxide

2018

J App Pharm Sci

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Synthesis of phosphorus heterocycles has gained increasing interest in recent years. Here we wish to report our results on synthesis and on studying of biological activity of 4-bromo-5-ethyl-2-(ethylamino)-5-methyl-5H-1,2-oxaphosphole 2-oxide (Br-oxph-1) using in vivo and in vitro approaches. Br-oxph-1 represents a structural analogue of 4-bromo-2-(diethylamino)-5-ethyl-5-methyl-5H-1,2-oxaphosphole 2-oxide (Br-oxph). Root growth inhibition test using Triticum aestivum L. seeds was provided in order to determine general toxicity of Br-oxph-1. The effective concentration that cause 50% of root length as compared to control was determined -8.23 × 10 3 μM. Cytotoxic potential of Br-oxph-1 (8.23 × 10 3 μM) was evaluated using Allium cepa L.-test. Significant decline in mitotic activity and disturbances of cell cycle kinetics were observed. Further, effects of Br-oxph-1 on human hepatoma cell line SK-HEP-1 in vitro were evaluated by means of cell proliferation assay. The results revealed concentration-dependent and statistically significant inhibitory effect. Concentration of Br-oxph-1 showing 50% reduction in cell proliferation/ viability was 0.642 × 10 3 µM. In conclusion, plant test-systems used in this study revealed growth inhibitory and cytostatic effects of Br-oxph-1. Data in vitro was in accordance with these results. Results of present study revealed stronger cytostatic effect of Br-oxph-1 in comparison with Br-oxph. Obviously, modifications in chemical structure of Br-oxph influence its biological effects.

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“…For toxicity test, seeds of T. aestivum were used. T. аestivum is proved to be appropriate test object in toxicity assays [30] [31]. Results of our previous study (data not shown) revealed that A. vernalis at concentration used in traditional medicine (hot extract, 6.67 g/l) inhibited the root growth of T. аestivum seeds by 78.31% in comparison to untreated control.…”

Section: Root Growth Inhibition Test and Determination Of Effective Cmentioning

confidence: 99%

Allelopathic Effects of Adonis vernalis L.: Root Growth Inhibition and Cytogenetic Alterations

Dragoeva¹,

Koleva²,

Nanova³

et al. 2015

JACEN

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A possible alternative to synthetic agricultural chemicals is through the use of allelopathy. Adonis species are rich sources of secondary metabolites. Such allelochemicals offer potential for the development of future pesticides. Allelochemicals influence plant growth and cause morphological alterations. This visible effect could be due to primary effects at cellular or molecular level. Changes in the mitotic activity and disturbances in different phases of mitotic division are accepted as indicators of cytotoxic influence. Mitotic abnormalities and induction of micronuclei in interphase cells are parameters used to determine genotoxicity. The purpose of the current study was to establish the possible allelopathic effect of Adonis vernalis L. water extracts through evaluation of root growth inhibition effect and cytogenetic alterations. Adonis vernalis L. growing wild in Bulgaria was used in the present study. Two types of water extracts were prepared: Hot and Cold Water Extract of A. vernalis (HWЕА and СWЕА). A 72-h root growth inhibition test was provided in order to determine the toxicity level of extracts. EC50 values were determined. For toxicity test, seeds of Triticum aestivum L. cv. GTW were used. Cytotoxic and genotoxic potential of water extracts (EC50) were evaluated using Allium cepa L.-test. The EC50 for HWEA and CWEA was determined 1.83 g/l and 0.78 g/l respectively. Significant influence on mitotic activity values and a marked decrease in percentage of telophase cells were observed after treatment with both extracts. Adonis extracts also induced different mitotic abnormalities in root-tip cells of Allium cepa L. The percent of interphase cells with micronuclei increased significantly only after treatment with HWEA. The results indicated growth inhibitory, cytotoxic and genotoxic effects of crude water extracts of A. vernalis L. These effects demonstrated the presence of water soluble allelochemicals in Adonis aerial parts.

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The genotoxic effects of logran onHordeum vulgareL. andTriticum aestivumL.

Cited by 10 publications

References 33 publications

Allelopathic activity of Nepeta nuda L. subsp. nuda water extracts

Allelopathic activity of Nepeta nuda L. subsp. nuda water extracts

Synthesis and Cytostatic Activity of 4-bromo-5-ethyl-2-(ethylamino)- 5-methyl-5H-1,2-oxaphosphole 2-oxide

Allelopathic Effects of Adonis vernalis L.: Root Growth Inhibition and Cytogenetic Alterations

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