The genomic sequence and comparative genomic analysis of cultivated passion fruit(Passiflora edulis L.)

Abstract

Cultivated passion fruit is a fruit tree widely cultivated in southern China, 24 but little is known about its genomics, which seriously restricts the molecular genetics 25 research of passion fruit. In this study, we analyzed the 165.7Mb representative 26 genome sequences. The results showed that the passion fruit genome contained a large 27 number of simple sequence repeats (SSR). Compared to the cassava and peach 28 genomes, the passion fruit genome has 23,053 predicted genes. These genes can be 29 aligned to 282 plant genomes. GO annotation indicated that these genes are involved in 30 metabolic pathways of carbohydrates, organic acids, lipids and other molecules. KEGG 31 pathway enrichment assigned these genes into five major categories and 19 secondary 32 functions. Cluster analysis of gene families showed that 12,767 genes could be 33 clustered into 9,868 gene families and 291 unique gene families. On the evolutionary 34 relationship, the passion fruit is closely related to Populus trichocarpa and Ricinus 35 communis, but the rate of evolution is slower. In summary, this genomic analysis result 36 is informative, and will facilitate the future studies on gene functions of passion fruit. 37

Identification, characterization and expression analysis of passion fruit (Passiflora edulis) microRNAs

“…It has been confirmed that several conserved, as well as speciesspecific miRNAs, are very important for plants in different biological processes and hence, profiling miRNAs in a non-model plant is essential to understand the regulation of various biological phenomena. With the recent draft genome sequence available (Wu et al 2019), the current study offers an insight of miRNAs and their respective targets in passion fruit using bioinformatics as well as wet-lab approach for better understanding the physiological processes in this plant. In summary, in this study for the first time, we have generated the miRNA and respective target profile of passion fruit and performed a comparative miRNA expression study between vegetative and reproductive tissues (leaves and fruits) as well as between two different varieties (yellow and purple skins).…”

“…In this study, web tool psR-NATarget (http://plant grn.noble .org/psRNA Targe t/?dowha t=Help) was used to identify the potential miRNA targets of passion fruit. Due to unavailability of passion fruit protein database in the psRNATarget web server, target search was conducted against the protein database of Populus trichocarpa, which is considered as the evolutionary closest species of passion fruit (Wu et al 2019). The parameters were adjusted manually, such as maximum expectation value of 3, translation inhibition ranges of 9-11 nt, number of top targets of 10, penalty for G:U pair of 0.5, and number of mismatches allowed in seed region of 1.5.…”