The Genomes of Acetic Acid Bacteria

Ehrenreich, Armin; Liebl, Wolfgang

doi:10.1007/978-3-319-60021-5_20

Cited by 3 publications

(3 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In parallel, phosphorylative dissimilation of glycerol can be carried out in the cytoplasm, where this substrate is oxidized by NAD(P) + −dependent enzymes . Unlike membrane‐bound dehydrogenases, their cytoplasmic counterparts lead to reversible reactions . The influence of extracellular pH on glycerol metabolism in G. oxydans has been already studied.…”

Section: Resultsmentioning

confidence: 99%

Dihydroxyacetone production from glycerol using Gluconobacter oxydans: Study of medium composition and operational conditions in shaken flasks

Morena

Acedos

Santos

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

The production of dihydroxyacetone from glycerol employing aerobic cultures of Gluconobacter oxydans is studied. Dihydroxyacetone is one of the most important value‐added products obtained from glycerol, a by‐product of biodiesel production. The effect of organic nitrogen source and initial substrate concentrations has been studied together with the possibility of product inhibition. Afterward, the influence of the main operating conditions (temperature, shaking speed, and initial biomass concentration) on in vivo glycerol dehydrogenase activity has also been considered. The results show no evidence of glycerol inhibition, but an important product inhibition was detected, which has been taken into account in a kinetic model for enzymatic activity description. In terms of operating conditions, pH was found to exert a great impact on glycerol conversion, being necessary to keep it above 4 to ensure complete glycerol conversion. The minimum temperature that maximized enzymatic activity was found to be 30°C. In addition, a surprising decoupling between biomass concentration and dihydroxyacetone production rate was observed when adding increasing nitrogen source concentrations at a fixed shaking speed. Glycerol dehydrogenase activity remains constant despite the increase in biomass concentration, contrary to what would be expected. This fact revealed the existence of a rate limiting factor, identified subsequently as oxygen transfer rate depending on the biomass concentration.

show abstract

Section: Resultsmentioning

confidence: 99%

Dihydroxyacetone production from glycerol using Gluconobacter oxydans: Study of medium composition and operational conditions in shaken flasks

Morena

Acedos

Santos

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…The results were contradictory to each other. Gluconobacter oxydans could gain energy from the incomplete oxidation of glucose to GA (Ehrenreich and Liebl, 2017). However, it could not obtain enough energy from the incomplete oxidation process due to the high osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

“…Gluconobacter oxydans (G. oxydans), an obligate aerobic Gram-negative bacterium belonging to the family Acetobacteraceae, is common in the sugar-enriched environments, such as nectars, flowers, fruits, and fermented foods (Raspor and Goranovič, 2008;Qin et al, 2021). Its respiratory metabolism is characterized by rapid selective oxidation of oxy-compounds to obtain energy from incomplete oxidation (Ehrenreich and Liebl, 2017;Lynch et al, 2019). The catalytic reaction center of the membrane-bound dehydrogenases participating in the incomplete oxidation is directed toward the periplasmic space, making substrate and product transport through cell membrane unnecessary (Deppenmeier et al, 2002;Keliang and Dongzhi, 2006;Gao et al, 2020) and rapid accumulation of incompletely 10.3389/fmicb.2022.977024 Frontiers in Microbiology 02 frontiersin.org oxidized product in the medium (Keliang and Dongzhi, 2006;Zhou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose

Liu

Wang²,

Xiao³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed efficiently in a sealed and compressed oxygen-supplying bioreactor. Therefore, the present study seeks to explore the osmotic stress tolerance against extra-high titer of representative lignocellulosic sugars like glucose. Gluconobacter oxydans can well adapted and fermented with initial 600 g/L glucose, exhibiting the highest bio-tolerance in prokaryotic strains and the comparability to the eukaryotic strain of Saccharomyces cerevisiae. 1,432 differentially expressed genes corresponding to osmotic pressure are detected through transcriptome analysis, involving several genes related to the probable compatible solutes (trehalose and arginine). Gluconobacter oxydans obtains more energy by enhancing the substrate-level phosphorylation, resulting in the increased glucose consumption rate after fermentation adaption phase. This study will provide insights into further investigation of biological tolerance and response to extra-high titers of glucose of G. oxydans.

show abstract

Producing Acetic Acid of Acetobacter pasteurianus by Fermentation Characteristics and Metabolic Flux Analysis

Yao

Liu

et al. 2018

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The acetic acid bacterium Acetobacter pasteurianus plays an important role in acetic acid fermentation, which involves oxidation of ethanol to acetic acid through the ethanol respiratory chain under specific conditions. In order to obtain more suitable bacteria for the acetic acid industry, A. pasteurianus JST-S screened in this laboratory was compared with A. pasteurianus CICC 20001, a current industrial strain in China, to determine optimal fermentation parameters under different environmental stresses. The maximum total acid content of A. pasteurianus JST-S was 57.14 ± 1.09 g/L, whereas that of A. pasteurianus CICC 20001 reached 48.24 ± 1.15 g/L in a 15-L stir stank. Metabolic flux analysis was also performed to compare the reaction byproducts. Our findings revealed the potential value of the strain in improvement of industrial vinegar fermentation.

show abstract

The Genomes of Acetic Acid Bacteria

Cited by 3 publications

References 80 publications

Dihydroxyacetone production from glycerol using Gluconobacter oxydans: Study of medium composition and operational conditions in shaken flasks

Dihydroxyacetone production from glycerol using Gluconobacter oxydans: Study of medium composition and operational conditions in shaken flasks

Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose

Producing Acetic Acid of Acetobacter pasteurianus by Fermentation Characteristics and Metabolic Flux Analysis

Contact Info

Product

Resources

About