The Genome-Wide Expression Profile of Saussurea lappa Extract on House Dust Mite-Induced Atopic Dermatitis in Nc/Nga Mice

Abstract

Saussurea lappa has been reported to possess anti-atopic properties. In this study, we have confirmed the S. lappa’s anti-atopic properties in Nc/Nga mice and investigated the candidate gene related with its properties using microarray. We determined the target gene using real time PCR in in vitro experiment. S. lappa showed the significant reduction in atoptic dermatitis (AD) score and immunoglobulin E compared with the AD induced Nc/Nga mice. In the results of microarray using back skin obtained from animals, we found that S. lappa’s properties are closely associated with cytokine-cytokine receptor interaction and the JAK-STAT signaling pathway. Consistent with the microarray data, real-time RT-PCR confirmed these modulation at the mRNA level in skin tissues from S. lappa-treated mice. Among these genes, PI3Kca and IL20Rβ were significantly downregulated by S. lappa treatment in Nc/Nga mouse model. In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro. Therefore, our study suggests that PI3Kca-related signaling is closely related with the protective effects of S. lappa against the development of atopic-dermatitis.

Review—Current Concepts in Inflammatory Skin Diseases Evolved by Transcriptome Analysis: In-Depth Analysis of Atopic Dermatitis and Psoriasis

“…The validation of murine models in AD studies is highly needed as no standardized model is currently existing which mimics the highly diverse pathomechanisms (inside-out vs. outside-in theory) [215]. Current AD models include cytokine overexpression (e.g., IL-4 [216] and IL-31), genetic mutations leading to dysfunctional protein expression (e.g., Filaggrin f t /flaky tail mice), NC/Nga model [76,217], PAR2 overexpression and house dust mite (HDM) application [215] and Aspergillus fumigatus-induced AD [200,218]. Formerly used in AD research, the topical application of SDS rather induces characteristics of contact dermatitis [215].…”

SPRR2C, DEFB4A, WIF1, CRY2, and KRT19 are correlated with the development of atopic eczema

“…Cytokine-cytokine receptor interaction is associated with the progression of skin-related diseases by regulating the proliferation of skin-derived precursors (SKPs) and SKP differentiation [43]. In addition, cytokine-cytokine receptor interaction has previously been demonstrated to play an important role in the progression of AE [44]. Thus, we inferred that cytokine-cytokine receptor interaction might be closely correlated with the AE progression.…”

Tenebrio molitor Larva Trypsin Hydrolysate Ameliorates Atopic Dermatitis in C57BL/6 Mice by Targeting the TLR-Mediated MyD88-Dependent MAPK Signaling Pathway

“…Moreover, when high levels of TMTH (10 mg/kg) were administered, the serum IgE level was similar to that of the positive control prednisolone. In the current study, prednisolone was administered at a dose of 3 mg/kg, considering similar studies carried out with prednisolone [ 32 , 33 , 34 , 35 , 36 ]. Thus, TMTH could be effectively utilized as a potential remedy for AD management.…”