The genome sequence and proteome of bacteriophage ΦCPV1 virulent for Clostridium perfringens

Volozhantsev, Nikolay V.; Verevkin, Vladimir V.; Bannov, Vasily A.; Krasilnikova, Valentina M.; Myakinina, Vera P.; Zhilenkov, E. L.; Svetoch, E. A.; Stern, Norman J.; Oakley, Brian B.; Seal, Bruce S.

doi:10.1016/j.virusres.2010.11.012

Cited by 27 publications

(23 citation statements)

References 51 publications

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“…Five ORFs were identified as structural protein genes, encoding products for the major capsid (ORF-10), tail (ORF-13), connector (ORF-16), collar (ORF-17) and pre-neck appendage (ORF-21) proteins. All putative structural proteins had highest similarity with Clostridium phage UCPV1 [7], ranging from 51.0 to 62.8% amino acid similarity. The pre-neck appendage proteins of UCP24R and UCPV1 have low global alignment similarity to their closest BLASTp hit (Bacillus phage GA-1, accession NP_073695, 28.1%), but they share a common protein domain architecture (Pfam domain PF12708) with the pre-neck appendages of six other podoviruses, including Bacillus phage U29.…”

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Complete genome sequence of the podoviral bacteriophage ΦCP24R, which is virulent for Clostridium perfringens

et al. 2012

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Bacteriophage ΦCP24R was isolated from raw sewage from a waste treatment plant, and lytic activity was observed against a type A Clostridium perfringens isolate. Electron microscopy revealed a small virion (44-nm-diameter icosahedral capsid) with a short, non-contractile tail, indicative of a member of the family Podoviridae. The phage had a linear, double-stranded DNA genome of 18,919 base pairs (bp) with 41 bp inverted terminal repeats and a type B DNA polymerase, which are characteristics of members of the subfamily Picovirinae. Out of 22 predicted genes in the genome, ten had significant sequence similarity to proteins of known function. Three distinct genes with lytic domains were identified, including a zinc carboxypeptidase domain that has not been previously reported in viruses. The ΦCP24R genome described herein is only the second Clostridium perfringens podovirus genome reported to date.

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Complete genome sequence of the podoviral bacteriophage ΦCP24R, which is virulent for Clostridium perfringens

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“…A virulent short-tailed bacteriophage ΦCPV1 was isolated in the Russian Federation utilizing C. perfringens as the host and was classified in the family Podoviridae (Volozhantsev et al, 2011). The purified virus had an icosahedral head and collar of approximately 42 and 23 nm in diameter, respectively, with a structurally complex tail of 37 nm lengthwise and a basal plate of 30 nm (Figure 1C).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, the viruses were purified by isopycnic centrifugation and analyzed by SDS-PAGE followed by mass spectrometry of the proteins. Subsequent identification of the phage genomic open reading frame ( ORF ) descriptive bacteriophage structure and identification of potential lytic proteins was completed by full-genome nucleotide sequencing, genome annotations, and BLAST analyses as described in detail by the investigators (Seal et al, 2011; Oakley et al, 2011; Volozhantsev et al, 2011, 2012; Morales et al, 2012). Recombinant proteins were produced by cloning bacteriophage genes encoding putative lysins such as N -acetylmuramoyl-l-alanine amidases and expression in E. coli (Simmons et al, 2010, 2012).…”

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confidence: 99%

Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium

Seal

2013

Poultry Science

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There has been a resurgent interest in the use of bacteriophages or their gene products to control bacterial pathogens as alternatives to currently used antibiotics. Clostridium perfringens is a gram-positive, spore-forming anaerobic bacterium that plays a significant role in human foodborne disease as well as nonfoodborne human, animal, and avian diseases. Countries that have complied with the ban on antimicrobial growth promoters in feeds have reported increased incidences of C. perfringens-associated diseases in poultry. To address these issues, new antimicrobial agents, putative lysins encoded by the genomes of bacteriophages, are being identified in our laboratory. Poultry intestinal material, soil, sewage, and poultry processing drainage water were screened for virulent bacteriophages that could lyse C. perfringens and produce clear plaques in spot assays. Bacteriophages were isolated that had long noncontractile tails, members of the family Siphoviridae, and with short noncontractile tails, members of the family Podoviridae. Several bacteriophage genes were identified that encoded N-acetylmuramoyl-l-alanine amidases, lysozyme-endopeptidases, and a zinc carboxypeptidase domain that has not been previously reported in viral genomes. Putative phage lysin genes (ply) were cloned and expressed in Escherichia coli. The recombinant lysins were amidases capable of lysing both parental phage host strains of C. perfringens as well as other strains of the bacterium in spot and turbidity reduction assays, but did not lyse any clostridia beyond the species. Consequently, bacteriophage gene products could eventually be used to target bacterial pathogens, such as C. perfringens via a species-specific strategy, to control animal and human diseases without having deleterious effects on beneficial probiotic bacteria.

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“…5). Host specificity has routinely been observed relative to the bacteriophages isolated from various C. perfringens isolates that is most likely due to evolution of the receptor and anti-receptor molecules Volozhantsev et al, 2011;Oakley et al, 2011). Therefore, selection of appropriate 'bacteriophage cocktails' may not necessarily be effective against many of the various bacterial isolates that exist in the environment and cause disease.…”

Section: Discussionmentioning

confidence: 99%

“…The major tail protein represented approximately 12.7% of the total protein, with an apparent size of 27 kDa while a minor structural protein composing 2.1% of the virion protein was reported with a predicted size of 55.1kD. More recently the proteins of virulent bacteriophages infecting C. perfringens have been described in detail Volozhantsev et al, 2011). From the siphoviruses , four principle virion protein regions were identified (Fig.…”

Section: Characteristics Of Clostridium Perfringens Bacteriophage Andmentioning

confidence: 99%

Bacteriophages of Clostridium perfringens

Seal¹,

Volozhantsev²,

Oakley³

et al. 2012

Bacteriophages

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The genome sequence and proteome of bacteriophage ΦCPV1 virulent for Clostridium perfringens

Cited by 27 publications

References 51 publications

Complete genome sequence of the podoviral bacteriophage ΦCP24R, which is virulent for Clostridium perfringens

Complete genome sequence of the podoviral bacteriophage ΦCP24R, which is virulent for Clostridium perfringens

Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium

Bacteriophages of Clostridium perfringens

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