The Genome of Human Herpesvirus 6: Maps of Unit-length and Concatemeric Genomes for Nine Restriction Endonucleases

Martin, M.; Thomson, Brian J.; Honess, R. W.; Craxton, Molly; Gompels, Ursula A.; Liu, M Y; Littler, Edward; Arrand, John R.; Teo, Ian; Jones, Michael D.

doi:10.1099/0022-1317-72-1-157

Cited by 111 publications

(106 citation statements)

References 42 publications

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“…It is possible that the replicating concatemeric DNAs are further matured during packaging producing the juxtaposed DRs which are then cleaved at the pac-2-pac-1 signals. Indeed several groups have found HHV-6 DR-DR junctions in their analyses (6,(31)(32)(33)(34). Furthermore, in additional experiments to be discussed elsewhere we also found packaged DNA containing double DRs in packaging compartments.…”

Section: Discussionmentioning

confidence: 99%

Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Borenstein

Frenkel

2009

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Borenstein

Frenkel

2009

Proc. Natl. Acad. Sci. U.S.A.

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“…Fourteen HHV-6 isolates were used for this study. HHV-6 (U1102) was cultivated as described (Martin et aL, 1991;Downing et al, 1987). HHV-6 (AJ) (Tedder et aL, 1987) was a gift from R. Tedder (Middlesex School of Medicine, London, U.K.).…”

Section: Methodsmentioning

confidence: 99%

“…Mutation rates are higher at these sites, presumably mediated by homologous recombination, and an example has been shown for the HHV-6 terminal repeats (Lindquester & Pellett, 1991). However, unlike EBV and HCMV HHV-6 does not contain extensive internal repeats (Martin et al, 1991). PCR amplification products from conserved genes without repeats in HCMV strains or selected regions in EBV strains can be grouped by restriction enzyme polymorphisms (Chou & Dennison, 1991;Sample et al, 1990).…”

Section: Relationships Between Hhv-6 Strain Variants and With Other Hmentioning

confidence: 99%

“…In contrast, the prototypes of HHV-6 group A (U 1102) and group B (Z29) show in addition to extensive DNA cross-hybridization an identical genome structure, base composition and size. These are AT-rich, 162 kbp genomes bounded by direct terminal repeats (Martin et al, 1991;Lindquester & Pellett, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…We are working on the Ul102 strain of HHV-6 (Downing et al, 1987), for which the only complete restriction enzyme fragment linkage map and set of clones is available (Martin et al, 1991). Sequencing studies of this virus DNA have shown that the organization of conserved genes and their encoded amino acid sequences are more related to those of human cytomegalovirus (HCMV) than those of the other human herpesviruses (Lawrence et al, 1990;Neipel et aL, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Gompels

Carrigan

Carss

et al. 1993

Journal of General Virology

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Fifteen human herpesvirus 6 (HHV-6) strain variants were analysed by PCR amplifications, restriction enzyme site polymorphism and sequence analyses. Three DNA regions were chosen for study: a fragment of a variable glycoprotein gene (210 bp), the conserved glycoprotein H (gH) gene complete with intergenic sequences (2381 bp) and the 5' intergenic region with the Nterminal coding sequence of gH up to a polymorphic BamHI site (427 bp). Infected cell DNA from five laboratory reference strains including GS, Ul102, AJ, Z29 and KF were examined together with DNA from peripheral blood lymphocytes infected with HHV-6 reactivated from blood and/or marrow from five bone marrow transplant (BMT) patients. Separate blood and marrow isolates were obtained from four BMT patients. In addition, HHV-6 sequences were examined directly from one of six Hodgkin's lymphomas and six B cell proliferations which contained HHV-6 DNA as detected by PCR amplification. The results show two groups of very closely related but heterogeneous strains which correlate with previous groupings by antigenic and restriction site differences. These are variant A strains (including laboratory strains GS, Ul102 and AJ) and variant B strains (including laboratory strains Z29 and KF, the Hodgkin's lymphoma strain, and the nine BMT patient isolates). Variations between the groups were 4 to 6 % in nucleotide sequence and 5 to 8.5 % in amino acid sequence. Within each group maximum heterogeneity was observed in different genes. Variant A strains differed by 2-0% in the variable glycoprotein gene sequence whereas variant B strains were identical in this region; conversely, variant B strains differed by 2 to 3 % in the gH N-terminal and intergenic sequences whereas variant A strains differed there by less than 0'2%. There was evidence for sequence drift independent of selection: relationships between the groups were shown by analyses of amino acid sequence, coding nucleotide sequence as well as intergenic sequence, and the B variant-specific BamHI site in the gH gene was due to a non-coding nucleotide substitution. There was little evidence for in vivo or in vitro variation: the gH nucleotide sequence from the uncultured lymphoma strain (first variant B gH gene identified) was almost identical to the gH sequence from four BMT isolates, and matched BMT isolates from blood and marrow were identical or with a single nucleotide substitution. The overall variation observed between the HHV-6 strain groups was similar or less than that seen between human cytomegalovirus strains such as AD169 or Towne, but deafly distinct from the much greater divergence between currently designated herpesvirus species.

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Human herpesvirus 6 in human immunodeficiency virus-infected individuals: Association with early histologic phases of lymphadenopathy syndrome but not with malignant lymphoproliferative disorders

et al. 1996

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The Genome of Human Herpesvirus 6: Maps of Unit-length and Concatemeric Genomes for Nine Restriction Endonucleases

Cited by 111 publications

References 42 publications

Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Human herpesvirus 6 in human immunodeficiency virus-infected individuals: Association with early histologic phases of lymphadenopathy syndrome but not with malignant lymphoproliferative disorders

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