The genome and proteome of Serratia bacteriophage η which forms unstable lysogens

Denyes, Jenna; Krell, Peter J.; Manderville, Richard A.; Ackermann, Hans-Wolfgang; She, Yi‐Min; Kropinski, Andrew M.

doi:10.1186/1743-422x-11-6

Cited by 16 publications

(12 citation statements)

References 54 publications

(59 reference statements)

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“…These also harbored Mu-like phage gp36 and gp29 genes, which might play a role in virulence [ 33 ]. The phage genomes both possessed ParB homologs and two putative transposase genes, which might function in phage integration into the host genome [ 54 ]. Each phage genome contained a repressor (Cro/CI family) gene, which prevents transcription and translation of lysis and other late genes [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Genomic, proteomic and bioinformatic analysis of two temperate phages in Roseobacter clade bacteria isolated from the deep-sea water

et al. 2017

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BackgroundMarine phages are spectacularly diverse in nature. Dozens of roseophages infecting members of Roseobacter clade bacteria were isolated and characterized, exhibiting a very high degree of genetic diversity. In the present study, the induction of two temperate bacteriophages, namely, vB_ThpS-P1 and vB_PeaS-P1, was performed in Roseobacter clade bacteria isolated from the deep-sea water, Thiobacimonas profunda JLT2016 and Pelagibaca abyssi JLT2014, respectively. Two novel phages in morphological, genomic and proteomic features were presented, and their phylogeny and evolutionary relationships were explored by bioinformatic analysis.ResultsElectron microscopy showed that the morphology of the two phages were similar to that of siphoviruses. Genome sequencing indicated that the two phages were similar in size, organization, and content, thereby suggesting that these shared a common ancestor. Despite the presence of Mu-like phage head genes, the phages are more closely related to Rhodobacter phage RC1 than Mu phages in terms of gene content and sequence similarity. Based on comparative genomic and phylogenetic analysis, we propose a Mu-like head phage group to allow for the inclusion of Mu-like phages and two newly phages. The sequences of the Mu-like head phage group were widespread, occurring in each investigated metagenomes. Furthermore, the horizontal exchange of genetic material within the Mu-like head phage group might have involved a gene that was associated with phage phenotypic characteristics.ConclusionsThis study is the first report on the complete genome sequences of temperate phages that infect deep-sea roseobacters, belonging to the Mu-like head phage group. The Mu-like head phage group might represent a small but ubiquitous fraction of marine viral diversity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3886-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genomic, proteomic and bioinformatic analysis of two temperate phages in Roseobacter clade bacteria isolated from the deep-sea water

et al. 2017

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“…All known Serratia phages belong to the order Caudovirales , classified into Myo - (twelve phages), Sipho - (five), Ackermann- (four), and Podoviridae (four) ( Table S1 ). Only two Serratia viruses were likely temperate—Eta [ 7 ] and Parlo [ 8 ]. Although the Eta phage produces turbid plaques, none of its encoded proteins are homologous to integrase and the CI repressor-like protein, Eta, therefore was not categorized as classically temperate [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Bujak

Decewicz

Kamiński

et al. 2020

IJMS

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Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages.

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“…To date, almost all phages examined that exhibit the Jersey-type morphotype have been restricted to Salmonella and Escherichia, the sole exception to this rule being Serratia phage η [15]. No phages with this morphotype have been observed to infect other Gram-negative genera, including Aeromonas, Pseudomonas, Vibrio, or Rhizobium (Table 1).…”

Section: Introductionmentioning

confidence: 99%

A proposed new bacteriophage subfamily: “Jerseyvirinae”

et al. 2015

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UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights.UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. PLEASE SCROLL DOWN FOR TEXT.A new bacteriophage subfamily -"Jerseyvirinae" the family Siphoviridae is proposed, named the "Jerseyvirinae" and consisting of three genera, the 36

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The genome and proteome of Serratia bacteriophage η which forms unstable lysogens

Cited by 16 publications

References 54 publications

Genomic, proteomic and bioinformatic analysis of two temperate phages in Roseobacter clade bacteria isolated from the deep-sea water

Genomic, proteomic and bioinformatic analysis of two temperate phages in Roseobacter clade bacteria isolated from the deep-sea water

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

A proposed new bacteriophage subfamily: “Jerseyvirinae”

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