The Genetics of Basidiomycetes

Papazian, Haig P.

doi:10.1016/s0065-2660(08)60159-5

Cited by 25 publications

(9 citation statements)

References 31 publications

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“…versieolor the 'nuclear replacement reaction' that follows fusion can be defined as a precise series of events (see Aylmore &' Todd, 1984a;Todd & Aylmore, 1985). Similar behaviour is probably associated with fusion in Coprinus cinereus (Bensaude,191 8 ; cited in Papazian, 1958) and Typhula trifolii (Noble, 1937). However, the complexity of the multinucleate Compartments of P. velutina makes a similarly detailed analysis of post-fusion behaviour difficult, but from this work several features were noted.…”

Section: Hyphal Fusions In Phanerochaete Velutinasupporting

confidence: 55%

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

Aylmore

Todd

1986

Microbiology

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Hyphal fusions forming in secondary mycelia of Phanerochaete velutina were examined using combined light and electron microscopy. Only 'self' fusions, occurring between hyphal compartments derived from the same colony, were studied. At points of contact, fusions formed from a single opening which expanded radially by highly localized lysis of the surrounding cell wall. Fusion pore enlargement failed to reach the full hyphal diameter and a rim of undissolved cell wall remained marking the original point of contact. Within 2 h of this, the compartments became re-partitioned by septum formation, synthesis being associated with mitotic division and beginning precisely at the site of fusion pore expansion. Septa formed at fusions showed identical structure and development to those occurring in unfused compartments.

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Section: Hyphal Fusions In Phanerochaete Velutinasupporting

confidence: 55%

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

Aylmore

Todd

1986

Microbiology

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“…Using methods described by Papazian (15), it is possible to calculate the expected number of nonparental ditype (NPD) tetrads resulting from double COs in a single interval using the frequency of single COs, which are observed as tetratypes (15). Applying this method to our data set, we would expect to observe 19 and 304 NPDs for I1 and I3, respectively; we observed only 2 and 51.…”

Section: Resultsmentioning

confidence: 99%

Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis

Francis

Lam²,

Harrison³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

180

197

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Recombination, in the form of cross-overs (COs) and gene conversion (GC), is a highly conserved feature of meiosis from fungi to mammals. Recombination helps ensure chromosome segregation and promotes allelic diversity. Lesions in the recombination machinery are often catastrophic for meiosis, resulting in sterility. We have developed a visual assay capable of detecting Cos and GCs and measuring CO interference in Arabidopsis thaliana. This flexible assay utilizes transgene constructs encoding pollen-expressed fluorescent proteins of three different colors in the qrt1 mutant background. By observing the segregation of the fluorescent alleles in 92,489 pollen tetrads, we demonstrate (i) a correlation between developmental position and CO frequency, (ii) a temperature dependence for CO frequency, (iii) the ability to detect meiotic GC events, and (iv) the ability to rapidly assess CO interference.cross-over ͉ meiosis ͉ tetrad ͉ gene conversion ͉ interference

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“…Additional 2-factor cross data (Table 3) show phase II temperature-dependent increase in recombination for other intervals spanning the ULG. On the other hand, map intervals between markers on linkage group IX (pf16 and pf 13) and on II (pf12 and pf18) show no temperature dependence.…”

mentioning

confidence: 92%

“…However, Papazian (13) has derived the following equation for assessing interference from 2-factor cross data. Recombination Frequencies Between Loci on the ULG Are Temperature Sensitive.…”

mentioning

confidence: 99%

Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii.

Ramanis¹,

Luck²

1986

Proc. Natl. Acad. Sci. U.S.A.

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The unil mutant of Chlamydomonas reinhar*i lacks one of the paired flagella seen in wild-type cells.The missing flagellum is cis to the eye spot and at this site the basal body is incomplete, lacking a transition zone. Together with nine other loci affecting flagellar assembly and two loci affecting flagellar function unil defines an '100-centinorgan linkage group with a circular map. Measurements of gene-centromere distances on the uni linkage group are consistent with the ordering of loci determined by recombination analysis and place the centromere near pf29, a motilitydefective mutant. In well-synchronized meiotic cycles, recombination frequencies between loci on the uni linkage group but not loci on other linkage groups show striking temperature sensitivity during a 1-to 2-hr period 5 days before meiosis. The mutant strain pf7 has short flagellar stubs; pf8 totally lacks flagella. Mutants pf10 andpf29 have full-length flagella but the mutant cells exhibit abnormal motility. Cells ofpf 10 show little linear movement and tend to travel in small irregular circles; in liquid culture they form a tight pellet.

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The Genetics of Basidiomycetes

Cited by 25 publications

References 31 publications

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis

Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii.

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