The genetics and genomics of the drought response in <i>Populus</i>

Street, Nathaniel R.; Skogström, Oskar; Sjödin, Andreas; James, Tucker; Rodríguez-Acosta, Maricela; Nilsson, Peter; Jansson, Stefan; Taylor, Gail

doi:10.1111/j.1365-313x.2006.02864.x

Cited by 212 publications

(187 citation statements)

References 76 publications

(134 reference statements)

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“…The fifth leaf below the first fully unfurled leaf was frozen in liquid N 2 for RNA extraction. Total RNA was extracted as described in Street et al (2006). RNA concentration was determined using an Eppendorf Biophotometer (Eppendorf, Hamburg, Germany), and quality was assessed using a 1% (w/v) agarose gel.…”

Section: Qtl Mappingmentioning

confidence: 99%

The physiological, transcriptional and genetic responses of an ozone- sensitive and an ozone tolerant poplar and selected extremes of their F 2 progeny

Street

Tallis

James

et al. 2011

Environmental Pollution

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Ozone-responsive transcriptional changes and genetic control were studied in Populus plants with contrasting ozone sensitivity. a r t i c l e i n f o a b s t r a c tRelatively little is known about the transcriptional response or genetic control of response and adaptation of trees to tropospheric ozone exposure. Such understanding is needed as up to 50% of forests, globally, may be subjected to phytotoxic concentrations of ozone. The physiological, transcriptional and genetic response to ozone was examined in Populus trichocarpa and P. deltoides, which show extreme sensitivity and tolerance to ozone, respectively. Using an inbred F 2 mapping population derived from these two species, we mapped quantitative trait loci (QTL) for traits associated with ozone response, examined segregation of the transcriptional response to ozone and co-located genes showing divergent responses between tolerant and sensitive genotypes with QTL. QTL were identified linking detrimental effects of ozone with leaf and biomass traits and differential responses were found for key genes involved in ethylene production and response.

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Section: Qtl Mappingmentioning

confidence: 99%

The physiological, transcriptional and genetic responses of an ozone- sensitive and an ozone tolerant poplar and selected extremes of their F 2 progeny

Street

Tallis

James

et al. 2011

Environmental Pollution

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“…Association mapping using expression quantitative trait loci has identified candidate genes for important traits in tomato (Solanum lycopersicum; Baxter et al, 2005) and poplar (Populus spp. ; Street et al, 2006). In addition, comparing allele-specific expression among inbred parental varieties and F1 hybrids of reciprocal crosses has revealed deviations from dosage dependency and enabled analysis of imprinting in maize endosperm (Guo et al, 2003).…”

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Transcript Profiling by 3′-Untranslated Region Sequencing Resolves Expression of Gene Families

2007

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Differences in gene expression underlie central questions in plant biology extending from gene function to evolutionary mechanisms and quantitative traits. However, resolving expression of closely related genes (e.g. alleles and gene family members) is challenging on a genome-wide scale due to extensive sequence similarity and frequently incomplete genome sequence data. We present a new expression-profiling strategy that utilizes long-read, high-throughput sequencing to capture the information-rich 3#-untranslated region (UTR) of messenger RNAs (mRNAs). Resulting sequences resolve gene-specific transcripts independent of a sequenced genome. Analysis of approximately 229,000 3#-anchored sequences from maize (Zea mays) ovaries identified 14,822 unique transcripts represented by at least two sequence reads. Total RNA from ovaries of drought-stressed wild-type and viviparous-1 mutant plants was used to construct a multiplex cDNA library. Each sample was labeled by incorporating one of 16 unique three-base key codes into the 3#-cDNA fragments, and combined samples were sequenced using a GS 20 454 instrument. Transcript abundance was quantified by frequency of sequences identifying each unique mRNA. At least 202 unique transcripts showed highly significant differences in abundance between wild-type and mutant samples. For a subset of mRNAs, quantitative differences were validated by real-time reverse transcription-polymerase chain reaction. The 3#-UTR profile resolved 12 unique cellulose synthase (CesA) transcripts in maize ovaries and identified previously uncharacterized members of a histone H1 gene family. In addition, this method resolved nearly identical paralogs, as illustrated by two auxin-repressed, dormancyassociated (Arda) transcripts, which showed reciprocal mRNA abundance in wild-type and mutant samples. Our results demonstrate the potential of 3#-UTR profiling for resolving gene-and allele-specific transcripts.

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“…Many plants drop their leaves during hot dry summers by the process of leaf abscission to reduce the transpiration load on the plant and to ensure young tissues have adequate water and nutrients to survive (Street et al, 2006;Agustí et al, 2012). Abscission is the process enabling plants to discard unwanted organs in response to environmental stimuli or developmental timing (Tudela and Primo-Millo, 1992;Agustí et al, 2012;Liljegren, 2012;Niederhuth et al, 2013a).…”

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Core Mechanisms Regulating Developmentally Timed and Environmentally Triggered Abscission

Patharkar

Walker

2016

Plant Physiol.

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Drought-triggered abscission is a strategy used by plants to avoid the full consequences of drought; however, it is poorly understood at the molecular genetic level. Here, we show that Arabidopsis (Arabidopsis thaliana) can be used to elucidate the pathway controlling drought-triggered leaf shedding. We further show that much of the pathway regulating developmentally timed floral organ abscission is conserved in regulating drought-triggered leaf abscission. Gene expression of HAESA (HAE) and INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) is induced in cauline leaf abscission zones when the leaves become wilted in response to limited water and HAE continues to accumulate in the leaf abscission zones through the abscission process. The genes that encode HAE/HAESA-LIKE2, IDA, NEVERSHED, and MAPK KINASE4 and 5 are all necessary for drought-induced leaf abscission. Our findings offer a molecular mechanism explaining drought-triggered leaf abscission. Furthermore, the ability to study leaf abscission in Arabidopsis opens up a new avenue to tease apart mechanisms involved in abscission that have been difficult to separate from flower development as well as for understanding the mechanistic role of water and turgor pressure in abscission.

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The genetics and genomics of the drought response in Populus

Cited by 212 publications

References 76 publications

The physiological, transcriptional and genetic responses of an ozone- sensitive and an ozone tolerant poplar and selected extremes of their F 2 progeny

The physiological, transcriptional and genetic responses of an ozone- sensitive and an ozone tolerant poplar and selected extremes of their F 2 progeny

Transcript Profiling by 3′-Untranslated Region Sequencing Resolves Expression of Gene Families

Core Mechanisms Regulating Developmentally Timed and Environmentally Triggered Abscission

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