The genetic structure of the A mating-type locus of Lentinula edodes

Au, Chun Hang; Wong, Man Chun; Bao, Dapeng; Zhang, Meiyan; Song, Chunyan; Song, Wei; Law, Patrick Tik Wan; Kües, Ursula; Kwan, Hoi Shan

doi:10.1016/j.gene.2013.11.036

Cited by 25 publications

(32 citation statements)

References 48 publications

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“…Interestingly, comparison of HD1 and HD2 sequences from other mushrooms, of which multiple alleles of HD subloci had been reported, revealed large variations in the conservation levels of these proteins. HD1 and HD2 proteins of Lentinula edodes (Au et al 2014) showed high similarity (76.46–77.9%, Figure S10 and Figure S11). In contrast, HD1 and HD2 proteins in S. commune showed considerably lower similarity (38.85–58.89%, Figure S12, Figure S13, and Figure S14), while HD1 and HD2 proteins of different subloci in C. cinerea (Badrane and May 1999) showed strongly varying conservation levels, depending on the sublocus (39.26–70.3%, Figure S15 and Figure S16).…”

Section: Resultsmentioning

confidence: 99%

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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The initiation of sexual development in the important edible and medicinal mushroom Flammulina velutipes is controlled by special genes at two different, independent, mating type (MAT) loci: HD and PR. We expanded our understanding of the F. velutipes mating type system by analyzing the MAT loci from a series of strains. The HD locus of F. velutipes houses homeodomain genes (Hd genes) on two separated locations: sublocus HD-a and HD-b. The HD-b subloci contained strain-specific Hd1/Hd2 gene pairs, and crosses between strains with different HD-b subloci indicated a role in mating. The function of the HD-a sublocus remained undecided. Many, but not all strains contained the same conserved Hd2 gene at the HD-a sublocus. The HD locus usually segregated as a whole, though we did detect one new HD locus with a HD-a sublocus from one parental strain, and a HD-b sublocus from the other. The PR locus of F. velutipes contained pheromone receptor (STE3) and pheromone precursor (Pp) genes at two locations, sublocus PR-a and PR-b. PR-a and PR-b both contained sets of strain-specific STE3 and Pp genes, indicating a role in mating. PR-a and PR-b cosegregated in our experiments. However, the identification of additional strains with identical PR-a, yet different PR-b subloci, demonstrated that PR subloci can recombine within the PR locus. In conclusion, at least three of the four MAT subloci seem to participate in mating, and new HD and PR loci can be generated through intralocus recombination in F. velutipes.

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Section: Resultsmentioning

confidence: 99%

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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“…Interestingly, whereas synteny analysis of the surrounding regions indicates that the separation of the two HD subloci in S. commune emerged via transposition of the two gene clusters, the extant arrangement in F. velutipes was driven by two large inversions (95). Other exceptions to the typical MIP – HD1/HD2 – β-fg gene arrangement were observed in the cacao pathogen Moniliophthora roreri , where both MIP and β-fg are located 40 and 60 kb upstream of HD1 / HD2 (105), respectively, and in the Shiitake mushroom, Lentinula edodes , where MIP is distant to the HD locus (106). Gene order conservation at the P/R locus is also observed among Agaricomycetes, although to a lesser extent compared to the HD locus.…”

Section: Breeding Systems In the Basidiomycotamentioning

confidence: 99%

Fungal Sex: The Basidiomycota

et al. 2017

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SUMMARY Fungi of the Basidiomycota, representing major pathogen lineages and mushroom-forming species, exhibit diverse means to achieve sexual reproduction, with particularly varied mechanisms to determine compatibilities of haploid mating partners. For species that require mating between distinct genotypes, discrimination is usually based on both the reciprocal exchange of diffusible mating pheromones, rather than sexes, and the interactions of homeodomain protein signals after cell fusion. Both compatibility factors must be heterozygous in the product of mating, and genetic linkage relationships of the mating pheromone/receptor and homeodomain genes largely determine the complex patterns of mating-type variation. Independent segregation of the two compatibility factors can create four haploid mating genotypes from meiosis, referred to as tetrapolarity. This condition is thought to be ancestral to the basidiomycetes. Alternatively, co-segregation by linkage of the two mating factors, or in some cases the absence of the pheromone-based discrimination, yields only two mating types from meiosis, referred to as bipolarity. Several species are now known to have large and highly rearranged chromosomal regions linked to mating-type genes. At the population level, polymorphism of the mating-type genes is an exceptional aspect of some basidiomycete fungi, where selection under outcrossing for rare, intercompatible allelic variants is thought to be responsible for mating types that may number several thousand. Advances in genome sequencing and assembly are yielding new insights by comparative approaches among and within basidiomycete species, with the promise to resolve the evolutionary origins and dynamics of mating compatibility genetics in this major eukaryotic lineage.

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“…Interestingly, whereas synteny analysis of the surrounding regions indicates that the separation of the two HD subloci in S. commune emerged via transposition of the two gene clusters, the extant arrangement in F. velutipes was driven by two large inversions (95). Other exceptions to the typical MIP-HD1/HD2--fg gene arrangement were ob-served in the cacao pathogen Moniliophthora roreri, where both MIP and -fg are located 40 and 60 kb upstream of HD1/HD2 (105), respectively, and in the Shiitake mushroom, Lentinula edodes, where MIP is distant from the HD locus (106). Gene order conservation at the P/R locus is also observed among Agaricomycetes, although to a lesser extent compared to the HD locus.…”

Section: Tetrapolar Systems With Multiple Alleles In the Agaricomycetesmentioning

confidence: 99%