The Genetic Puzzle of Multicopy Genes: Challenges and Troubleshooting
Vania Gabriela Sedano Partida,
Henrique Moura Dias,
Maria Teresa Portes
et al.
Abstract:Background: Studies with multicopy genes impose challenges related to gene redundancy and sequence similarity among copies. Recent advances in molecular biology and genomics tools associated with dedicated databases facilitate their study. Thus, the present work emphasizes the need for rigorous methodologies and standardized approaches to interpret RT-qPCR results accurately. Results: The present work in Physcomitrium patens provides a comprehensive five-step protocol, using thiamine thiazole synthase (THI1) a… Show more
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