The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial parameters influencing survival of mice derived from embryonic stem cells by tetraploid embryo aggregation

Li, Xiangyun; Wei, Wei; Yong, Jun; Jia, Qiong; Yu, Yuansong; Di, Keqian

doi:10.1530/rep.1.00667

Cited by 19 publications

(15 citation statements)

References 26 publications

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“…Over the years, several studies have reported that chromosome make-up correlates with the capacity of ES cell clones to contribute to the formation of all tissues, including the germline, of the adult chimaeras. The data support the notion that karyological instability, and not loss of pluripotency, is the major reason for the lack of contribution to chimaeras of individual ES cell clones, and that karyotype analysis is a predictor of the germline transmission capacity of ES cell lines [30][31][32][33][34] . Some studies suggest that the long-term culture of iPS cells, similar to the situation for ES cells, has to be monitored carefully for culture-induced chromosomal abnormalities [35] .…”

Section: Chromosomal Abnormalitiessupporting

confidence: 71%

Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

Carstea

Pirity²,

Dinnyés³

2009

WJSC

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In mice, gene targeting by homologous recombination continues to play an essential role in the understanding of functional genomics. This strategy allows precise location of the site of transgene integration and is most commonly used to ablate gene expression ("knockout"), or to introduce mutant or modified alleles at the locus of interest ("knock-in"). The efficacy of producing live, transgenic mice challenges our understanding of this complex process, and of the factors which influence germline competence of embryonic stem cell lines. Increasingly, evidence indicates that culture conditions and in vitro manipulation can affect the germline-competence of Embryonic Stem cell (ES cell) lines by accumulation of chromosome abnormalities and/or epigenetic alterations of the ES cell genome. The effectiveness of ES cell derivation is greatly straindependent and it may also influence the germline transmission capability. Recent technical improvements in the production of germline chimeras have been focused on means of generating ES cells lines with a higher germline potential. There are a number of options for generating chimeras from ES cells (ES chimera mice); however, each method has its advantages and disadvantages. Recent developments in induced pluripotent stem (iPS) cell technology have opened new avenues for generation of animals from genetically modified somatic cells by means of chimera technologies. The aim of this review is to give a brief account of how the factors mentioned above are influencing the germline transmission capacity and the developmental potential of mouse pluripotent stem cell lines. The most recent methods for generating specifically ES and iPS chimera mice, including the advantages and disadvantages of each method are also discussed.

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Section: Chromosomal Abnormalitiessupporting

confidence: 71%

Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

Carstea

Pirity²,

Dinnyés³

2009

WJSC

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“…For example, outbred and hybrid tetraploid host embryos were found to be superior to inbred strain tetraploid host embryo tested with the same F1-hybrid ES cell line. 28 Apart from increased body weight, ES cell derived animals were shown to be phenotypically normal and not different from isogenic controls from normal mating. 57 This ability to derive embryos and mice directly from ES cells represents a unique method to study gene families and pathways and for rapid phenotype analysis.…”

Section: • Diploid-tetraploid Embryos • Es Cells-tetraploid Embryosmentioning

confidence: 99%

“…22,[26][27][28] This ability of tetraploid cells to persist in the embryo proper has important implications for experimental interpretation and is the basis for the recommendation that reporters such as EGFP or lacZ be used to distinguish cells originating the tetraploid host embryos from those derived from ES cells at later stages of gestation. 29,30 There are two main types of chimeras that can be generated with tetraploid embryos:…”

Section: Reviewmentioning

confidence: 99%

“…Characterized F1-hybrid ES cells introduced into tetraploid embryos can give rise to viable and fertile animals directly from ES cells and speed up traditional breeding. 28,32,33,[56][57][58][59] The genetic background of tetraploid embryos can also contribute to the survival of ES cell derived mice. For example, outbred and hybrid tetraploid host embryos were found to be superior to inbred strain tetraploid host embryo tested with the same F1-hybrid ES cell line.…”

Section: • Diploid-tetraploid Embryos • Es Cells-tetraploid Embryosmentioning

confidence: 99%

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Tetraploid Complementation Assay

Gertsenstein

2014

The Guide to Investigation of Mouse Pregnancy

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“…For the MA ES cell line series, we used outbred mice that were produced by crossing the 129 and BALB lines (hereafter, referred to as 1B), for the MC series-F 1 (1B 9 C57BL), and for MD-F 1 (1B 9 DD). We derived ES cells from hybrid embryos because such ES cells have higher chimerization efficiency compared to a linear genetic background (Eggan et al 2001(Eggan et al , 2002Li et al 2005).…”

Section: Es Cell Derivation and Culturementioning

confidence: 99%

Cytogenetic analysis and Dlk1-Dio3 locus epigenetic status of mouse embryonic stem cells during early passages

et al. 2014

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Mouse embryonic stem (ES) cells are widely used in early development studies and for transgenic animal production; however, a stable karyotype is a prerequisite for their use. We derived 32 ES cell lines of outbred mice (129 9 BALB (1B), C57BL 9 1B, and DD 9 1B F1 hybrids). Pluripotency was assessed by utilizing stem-cell-marker gene expression, teratoma formation assays and the formation of chimeras. It was shown that only 21 of the 32 ES cell lines had a diploid modal number of chromosomes of 40. In these lines, the percentage of diploid cells varied from 30.3 to 78.9 %, and trisomy of chromosomes 1, 8 and 11 was observed in some cells in 16.7, 36.7 and 20.0 % of the diploid ES cell lines, respectively. Some cells had trisomy of chromosomes 6, 9, 12, 14, 18 and 19. In situ hybridization with an X chromosome paint probe revealed that 7 of the 11 XXcell lines had X chromosome rearrangements in some cells. Analysis of the methylation status of the Dlk1-Dio3 locus showed that imprinting was altered in 4 of the 18 ES cell lines. Thus, mouse ES cell lines are prone to chromosome abnormalities even at early passages. Therefore, routine cytogenetic and imprinting analyses are necessary for ES cell characterization.

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The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial parameters influencing survival of mice derived from embryonic stem cells by tetraploid embryo aggregation

Cited by 19 publications

References 26 publications

Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

Tetraploid Complementation Assay

Cytogenetic analysis and Dlk1-Dio3 locus epigenetic status of mouse embryonic stem cells during early passages

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