The genetic control of bud formation from callus cultures of diploid alfalfa

Reisch, Bruce I.; Bingham, E. T.

doi:10.1016/0304-4211(80)90071-1

Cited by 104 publications

(50 citation statements)

References 14 publications

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“…Until now, genetic analysis of in-vitro shoot bud regeneration has been limited to observations of the degree of genetic variation in regeneration ability (Skvirsky et al 1984;Zelcer et al 1984;Koornneef et al 1987;Ezura et al 1993), and to the identification of specific genetic loci which appear to influence regeneration ability (Reich and Bingham 1980;Nadolska-Orczyk and Malepszy 1989;Dulieu 1991;Taylor and Veilleux 1992;Koornneef et al 1993;Birhman et al 1994). Here we describe experiments which compare the shoot-regeneration ability of callus tissue from phytohormone mutants (ga4 and gai) of Arabidopsis thaliana with that of wild-type [Landsberg erecta (Ler)] control callus tissue, ga4 is a dwarf mutant that is sensitive to exogenously applied gibberellin (GA; Koornneef and van der Veen 1980), and contains lower levels of endogenous biologically active GA species than does Ler (Talon et al 1990a).…”

Section: Introductionmentioning

confidence: 99%

Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh.

Ezura

Harberd

1995

Planta

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The regeneration of shoot buds from callus cells in vitro is an important technique in modern plant genetic manipulation. Whilst it is clear that genetic factors play a major role in determining the ability of callus cells to become organized into regenerating shoot buds, the precise nature of these factors remains unknown. Here we show that callus derived from mutants of Arabidopsis thaliana which have reduced levels of endogenous bioactive gibberellins (GAs), or reduced responsivity to GAs, regenerates shoot buds more readily than does callus derived from wild-type controls. In addition, exogenous GA reduces, and exogenous paclobutrazol (a GA-bio-synthesis inhibitor) increases, the frequency of shoot bud regeneration from wild-type callus. These results show that GA levels play a role in regulating shoot bud regeneration from callus, and suggest that variation in endogenous GA levels or responsivity may account for a major component of the genetic variation in shoot bud regeneration frequency described in other species.

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Section: Introductionmentioning

confidence: 99%

Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh.

Ezura

Harberd

1995

Planta

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“…It is known that the ability of alfalfa, Medicago sativa L., to form somatic embryos is genotype dependent and varies between the cultivars as well as amongst genotypes of a cultivar (5). It has been shown that the somatic embryogenesis character in alfalfa is controled by two independent, complementary, dominant genes (31,15,19,6).…”

Section: Introductionmentioning

confidence: 99%

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Barbulova¹,

Iancheva²,

Zhiponova³

et al. 2002

Biotechnology & Biotechnological Equipment

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“…In some cases, where reciprocal crosses (Dulieu, 1991;Ohki etal., 1978;Tomes and Smith, 1985) or cytoplasm substitutions were studied (Mathias etal., 1986), maternal effects were observed. In Petunia (Dulieu, 1991), tomato (Koornneef et a/., 1987), cucumber (Nadolska-Orczyk and Malepszy, 1989), and in alfalfa (Reich and Bingham, 1980), the regeneration capacity could be assayed as a qualitative trait in F2 and/or backcross populations. In these species, the favourable regeneration capacity was found to be controlled by dominant alleles at two distinct loci.…”

Section: Introductionmentioning

confidence: 99%

Characterization and mapping of a gene controlling shoot regeneration in tomato

Koornneef¹,

Bade

Hanhart³

et al. 1993

The Plant Journal

116

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SummaryThe superior regeneration capacity of Lycopersicon peruvianum was introduced into the cultivated tomato Lycopersicon esculentum by backcrossing hybrid material with the tomato genotype VF11. In segregating material derived from these backcrosses, the ability to regenerate shoots on root explants cultured on a zeatin-containing medium, was highly correlated with the ability to regenerate shoots on established callus cultures. The efficient shoot-regenerating root explant system permitted us to study the genetics of this trait and to locate the genes involved, using a set of morphological markers defining all 12 tomato chromosomes. Depending on the tomato genotype, mono, -di-or trigenic ratios were observed. It is concluded that a dominant L. peruvianurn allele at a locus (Rg-7) near the middle of chromosome 3 determines efficient shoot regeneration on root explants in tomato in combination with dominant alleles at one or two other loci of either L. peruvianum or L. esculentum origin. The map location of the Rg-7 locus was refined further using a number of chromosome-3-specific RFLPs. The addition of new classical and RFLP linkage data to existing literature data and subsequent processing resulted in a revised and integrated map of tomato chromosome 3. From a morphological and physiological analysis of genotypes differing in Rg phenotype, it is concluded that the genetic component associated with regeneration determines the maintenance of morphogenetic competence and not the sensitivity to hormones.

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The genetic control of bud formation from callus cultures of diploid alfalfa

Cited by 104 publications

References 14 publications

Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh.

Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh.

Establishment of Embryogenic Potential of Economically Important Bulgarian Alfalfa Cultivars (Medicago SativaL.)

Characterization and mapping of a gene controlling shoot regeneration in tomato

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