The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coli

An Electron Microscopic Study of Large Bacterial Polyribosomes

“…After centrifugation for 150 min in a Spinco Model L at 25,000 rpm in the SW 25 head, samples were collected through the bottom of the density gradient tube and pumped through a Gilford spectrophotometer for measurement of absorbance at 260 mp. Samples were also analyzed for /3-galactosidase activity by the method of Pardee, Jacob, and Monod (16).…”

On the specificity of cyclic AMP action in Escherichia coli

“…Glucose was added in a concentration of 1O-2 M, CAMP and its analogues were added in concentrations of 1O-3 M. The cultures were incubated and aliquots (0.5 ml) were withdrawn, chilled in ice and mixed with a drop of toluene and 100 ~1 of a deoxycholate solution (100 pg/ml). The induced @-galactosidase was determined spectrophotomitrically with o-nitrophenyl /3-D-galactopyranoside (Lachema, Czechoslovakia) as substrate [8].…”

Genetic Suppressors and Recovery of Repressed Biochemical Memory

“…In 1961, Arthur Pardee, my first postdoctoral mentor, gave me a project he had started that was to lead me to an appreciation of the power of suppressor analysis. Art had recently returned from a sabbatical at the Institut Pasteur in Paris, where along with François Jacob and Jacques Monod, he provided the crucial argument for repressor control of gene expression (5). After Art's lab moved from Berkeley to Princeton University, he came up with an idea that was inspired by the Pasteur group's characterization of the lac operator.…”