The Genetic Basis of Resistance to Diazinon in Natural Populations of Drosophila melanogaster

Pyke, Fiona M.; Bogwitz, Michael; Perry, Trent; Monk, Adrian C; Batterham, Philip; McKenzie, John A.

doi:10.1023/b:gene.0000019920.71944.2b

Cited by 27 publications

(30 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This strain expresses GAL4 in the gastric cecum, midgut, Malpighian tubules, and fat body of larvae and was used to drive Cyp6g1 in these tissues in crosses to UAS-Cyp6g1 strains. Insecticide resistance bioassays using dichloro-diphenyltrichloroethane (DDT), dicyclanil, and nitenpyram were performed using standard techniques (Daborn et al 2001;Pyke et al 2004;Magoc et al 2005). Survival to adulthood from five replicates of 50 larvae (first instar) per vial was used to assay for resistance to nitenpyram and dicyclanil.…”

Section: Methodsmentioning

confidence: 99%

Cis-Regulatory Elements in the Accord Retrotransposon Result in Tissue-Specific Expression of the Drosophila melanogaster Insecticide Resistance Gene Cyp6g1

et al. 2007

Self Cite

View full text Add to dashboard Cite

Transposable elements are a major mutation source and powerful agents of adaptive change. Some transposable element insertions in genomes increase to a high frequency because of the selective advantage the mutant phenotype provides. Cyp6g1-mediated insecticide resistance in Drosophila melanogaster is due to the upregulation of the cytochrome P450 gene Cyp6g1, leading to the resistance to a variety of insecticide classes. The upregulation of Cyp6g1 is correlated with the presence of the long terminal repeat (LTR) of an Accord retrotransposon inserted 291bp upstream of the Cyp6g1 transcription start site. This resistant allele (DDT-R) is currently at a high frequency in D. melanogaster populations around the world. Here, we characterize the spatial expression of Cyp6g1 in insecticide-resistant and -susceptible strains. We show that the Accord LTR insertion is indeed the resistance-associated mutation and demonstrate that the Accord LTR carries regulatory sequences that increase the expression of Cyp6g1 in tissues important for detoxification, the midgut, Malpighian tubules, and the fat body. This study provides a significant example of how changes in tissue-specific gene expression caused by transposableelement insertions can contribute to adaptation.

show abstract

Section: Methodsmentioning

confidence: 99%

Cis-Regulatory Elements in the Accord Retrotransposon Result in Tissue-Specific Expression of the Drosophila melanogaster Insecticide Resistance Gene Cyp6g1

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Natural mortality in control vials was taken into account when generating dosage mortality curves with the PRIPROBITNM program (M. Sakuma, Kyoto University, Kyoto) using a natural regression model (22). Resistance bioassays using dicyclanil, nitenpyram, and diazinon were performed by using standard techniques (23,24).…”

Section: Methodsmentioning

confidence: 99%

“…Level of Cyp12a5 (A) and Cyp12a4 (B) mRNA in third-instar larvae of NB16 compared with susceptible strains Canton-S and y; cn bw sp, as determined by real-time PCR (ϮSEM, n ϭ 3). All levels are relative to the susceptible strain Canton-S. Also shown are the mRNA levels of other lufenuron-resistant strains, WC2 and Inn5 (23). Compared with Canton-S, 4.2 times more Cyp12a4 mRNA is detected in NB16.…”

Section: Genetic Mapping Of Lufenuronmentioning

confidence: 99%

Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster

Bogwitz

Chung

Magoc

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

124

112

View full text Add to dashboard Cite

Lufenuron is an insect growth regulator insecticide mainly used for the control of the cat flea. To understand mechanisms of resistance to lufenuron, we have characterized lufenuron resistance in a natural population of Drosophila melanogaster. In this study we have used precise genetic mapping to identify a mechanism of lufenuron resistance: the overexpression of the cytochrome P450 gene Cyp12a4. Cyp12a4 is predicted to encode a mitochondrial cytochrome P450 enzyme. Expression of Cyp12a4 in D. melanogaster third-instar larvae was detected in the midgut and Malpighian tubules of both lufenuron-resistant and wild-type strains. The level of Cyp12a4 expression in the midgut is higher in the lufenuron-resistant strain than in wild-type strains. Driving the expression of Cyp12a4 in the midgut and Malpighian tubules by using the GAL4͞UAS gene expression system results in lufenuron resistance, but it does not result in resistance to three other insecticide classes. Transgenic expression of Cyp12a4 in a ubiquitous expression pattern results in late embryonic lethality, suggesting that high-level ectopic expression of Cyp12a4 is detrimental to development. cytochrome P450 ͉ insecticide resistance ͉ insect growth regulator ͉ genetic mopping I nsecticide resistance is an important example of natural selection (1). Resistance is predominantly mediated either by changes in the metabolism of the insecticide or by changes in the sensitivity of insecticide targets (2). Insecticide resistance mediated by target site modification is well documented for most commonly used insecticides (3-7). Molecular mechanisms of resistance due to increased insecticide metabolism by cytochrome P450s, esterases, and glutathione S-transferases are less well understood. Resistance is commonly found to be the result of up-regulation of single or multiple members of these enzyme families (8-13); however, resistance due to structural changes in enzymes has also been documented (14-16).Lufenuron is an insect growth regulator insecticide, mainly used in the control of the cat flea, Ctenocephalides felis. It is active against larval developmental stages, causing cuticular lesions resulting from the disruption of chitin synthesis (17). Lufenuron is also active against Diptera, including Drosophila melanogaster (18). To understand possible resistance mechanisms to lufenuron, we have used D. melanogaster as a model. Lufenuron resistance in D. melanogaster was originally reported in populations from two widely separated locations in the United States (19). As it was not expected that D. melanogaster had been in contact with lufenuron, it was postulated that this resistance resulted from cross-resistance that had evolved to an earlier, widely used insecticide (19). Subsequently, it was found that resistance to lufenuron was caused by the up-regulation of the cytochrome P450 gene Cyp6g1, which confers resistance to numerous insecticide classes, and that Cyp6g1-mediated resistance is widespread (8,20).Here we describe the discovery of a mechanism of lufenuron r...

show abstract

“…It was indicated above that the pesticides have no selectivity of action. Gene-modified types of plants are developed to enhance the resistance of agricultural plants to the action of specific pesticides (Christoffers et al, 2002;Pyke et al, 2004;Sakagami et al, 2005). Based on the results presented, we may assert that the use of pesticides should drastically be reduced.…”

Section: Pamphagus Hyalinusmentioning

confidence: 99%

Investigation of Degradation of Pesticide Lontrel in Aqueous Solutions

Saratovskih¹

2012

Herbicides - Environmental Impact Studies and Management Approaches

View full text Add to dashboard Cite

The Genetic Basis of Resistance to Diazinon in Natural Populations of Drosophila melanogaster

Cited by 27 publications

References 43 publications

Cis-Regulatory Elements in the Accord Retrotransposon Result in Tissue-Specific Expression of the Drosophila melanogaster Insecticide Resistance Gene Cyp6g1

Cis-Regulatory Elements in the Accord Retrotransposon Result in Tissue-Specific Expression of the Drosophila melanogaster Insecticide Resistance Gene Cyp6g1

Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster

Investigation of Degradation of Pesticide Lontrel in Aqueous Solutions

Contact Info

Product

Resources

About