The Genes Involved in Dentinogenesis

Chen, Shuang; Xie, Han; Zhao, Shouliang; Wang, Shuai; Wei, Xiaoling; Liu, Shang-feng

doi:10.1080/15476278.2021.2022373

Cited by 12 publications

(8 citation statements)

References 159 publications

(204 reference statements)

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“…Y1 and Y2 were in Cluster 3, which highly expressed genes of COL3A1 , FBN2 , and VCAN ( Figure 4C ; Supplementary Figure S4A ). These genes were mainly involved in dentin matrix formation ( Chen et al, 2022 ). PTN with a higher expression in Cluster 3 than in the other three groups ( Figure 4C ) might be associated with early mineralization of dentin ( Erlandsen et al, 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Ren¹,

Wen

Zhao³

et al. 2022

Front. Physiol.

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The dental pulp plays a crucial role in the long-term maintenance of tooth function. The progress of endodontic treatment and pulp tissue regeneration engineering has made pulp-regeneration therapy promising in clinical practice. However, the mechanisms of pulp regeneration and the role of dental stem cells in development and regeneration have not been fully elucidated. Bridging the gaps between clinical operation and basic research is urgently needed. With the application of single-cell sequencing technology in dental research, the landscapes of human dental pulp cells have begun being outlined. However, the specific cellular heterogeneity of dental pulp cells, especially that of dental stem cells, at different spatial and temporal levels, is still unclear. In this study, we used single-cell RNA sequencing analysis of pulp samples at four different developmental stages and combined the findings with immunohistochemical staining to explore the development of dental pulp and stem cells. The results revealed temporal changes in the proportion of pulp cells during development. For example, mononuclear phagocytes accounted for a higher proportion in early samples. Odontoblasts identified by DMP1 had a higher expression of ion channel-related and neurodevelopment-related genes. Subpopulations were identified in fibroblasts, odontoblasts, and mesenchymal stem cells. We identified a subclass of odontoblasts that expresses DGKI and RRBP1 present in early developmental samples. A population of earlier mesenchymal stem cells expressed the SEPTIN gene, which may have greater proliferative and differentiation potential. Furthermore, dental pulp stem cells can differentiate into two directions: mineralization and myogenesis. In summary, the specific cellular heterogeneity of dental pulp cells was revealed at different spatial and temporal levels. These findings may shed light on the mechanism of tooth development. The gene expression profile of developing pulp cells may help to select cells for regenerative engineering and improve the success of dental pulp regeneration.

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Section: Resultsmentioning

confidence: 99%

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Ren¹,

Wen

Zhao³

et al. 2022

Front. Physiol.

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“…The non-collagen proteins that form the dentin matrix are synthesized after the hydrolysis of DSPP secreted by odontoblasts [21,49]. DSPP deregulation damages dentin mineralization [50] and downregulates DSPP expression, causing abnormal dental pulp cell differentiation [51].…”

Section: Discussionmentioning

confidence: 99%

“…During the later stages of odontogenesis, the alveolar bone surrounding the tooth germ undergoes an intense remodeling process, which supports its eruption [54], responding to the signals from dental and periodontal tissues [55]. The RANKL/OPG system was analyzed due to the versatility of RANKL as a paracrine molecule acting as a receptor or being released into matrix [21,22]. Dental pulp cells and odontoblasts express RANKL and OPG [6,20,56], but their function during odontogenesis has not been well described.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies demonstrate a pivotal role played by the RANKL (receptor activator of nuclear factor-kappa beta ligand) and OPG (osteoprotegerin) system during dentinogenesis [6,20,21]. Moreover, the tooth-eruption process requires activation and recruitment of osteoclasts on the alveolar bone surface developing an eruption pathway regulated by the RANKL/OPG system [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Maxillary and dental development in the offspring of protein‐restricted female rats

Calsa

Bortolança

Masiero

et al. 2022

European J Oral Sciences

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Nutritional restriction during developmental periods impairs organ physiology. Female rats were subjected to protein restriction during pregnancy and lactation to analyze dental and maxillary development. Four exposure groups were considered: normal‐protein diet during pregnancy and lactation (NP, 17% casein), low‐protein diet during lactation (LP‐L, 6% casein), low‐protein diet during pregnancy and lactation (LP), and low‐protein diet during pregnancy (LP‐G). Maxillae from 15‐day‐old male pups were collected. All protein‐restricted groups presented increased dentin thickness and reduced alveolar bone area. When protein restriction was applied during both gestation and lactation (LP), harmful effects were observed in the form of loss of protective OPG (osteoprotegerin) in tooth epithelium‐mesenchyme, due to higher RANKL expression, delay in odontoblast maturation, less dental pulp vascularity, reduction in amount of alveolar bone, and less matrix mineralization. In the LP‐L group, effects of protein restriction seemed less harmful, and despite less alveolar bone, the enhancement in BMP‐7, VEGF, and RANKL seems a compensatory signal to maintain maxillary osteogenesis. In LP‐G animals, Dspp expression was higher, suggesting a delay in odontoblast maturation or expression recuperation. In conclusion, maternal protein restriction affects dental and maxillary development. A low‐protein diet only in gestation allows for normal development. A low‐protein diet during gestation‐lactation results in impaired odontogenesis that may increase susceptibility of dental anomalies.

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“…Organization of the enamel, dentin and the junction are directly connected to mechanical properties during all life and related to diet of each animal species. Mutations in genes involved in dentinogenesis and amelogenesis perturb this organization and affect the mechanical properties of the dental tissues, essential to mastication and nutrient digestion ( Chen et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Enamel and dentin in Enamel renal syndrome: A confocal Raman microscopy view

et al. 2022

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Enamel Renal Syndrome (ERS) is a rare genetic disorder caused by biallelic mutations in Family with sequence similarity 20A (FAM20A) gene encoding the secretory pathway pseudokinase FAM20A. ERS is characterized by hypoplastic amelogenesis imperfecta (AI), impaired tooth eruption, intra-pulpal calcifications, gingival fibromatosis and nephrocalcinosis of various severity. Previous studies showed that the hypoplastic enamel was also hypomineralized but its chemical composition has not been extensively studied. Furthermore it is currently unclear whether dentinal defects are associated with AI in ERS patients. The objective of the study was to provide a structural and chemical analysis of enamel, dentin and dentin enamel junction (DEJ) in ERS patients carrying four, previously reported, distinct mutations in FAM20A. Chemical cartography obtained with Raman microscopy showed that compared to control samples, ERS enamel composition was severely altered and a cementum-like structure was observed in some cases. Chemical composition of peripulpal dentin was also affected and usual gradient of phosphate intensity, shown in DEJ profile, was absent in ERS samples. DEJ and dentinal anomalies were further confirmed by scanning electron microscopy analysis. In conclusion, our study shows that enamel formation is severely compromised in ERS patients and provides evidence that dentinal defects are an additional feature of the ERS dental phenotype.

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The Genes Involved in Dentinogenesis

Cited by 12 publications

References 159 publications

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Maxillary and dental development in the offspring of protein‐restricted female rats

Enamel and dentin in Enamel renal syndrome: A confocal Raman microscopy view

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