The genes coding for histone H3 and H4 in<i>Neurospora crassa</i>are unique and contain intervening sequences

Woudt, Lambertus P.; Pastink, Albert; Kempers-Veenstra, A E; Jansen, Antonius E.M.; Mager, Willem H.; Planta, Rudi J.

doi:10.1093/nar/11.16.5347

Cited by 138 publications

(60 citation statements)

References 31 publications

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“…An additional consensus sequence, PuCTPuAC, was found within and near the 3' terminus of all intervening sequences of the glucoamylase gene. An identical sequence is similarly located within intervening sequences of other filamentous ascomycetes (T. reesei [54] and N. crassa [26,67]) and is related to the consensus sequence TACTAAC found within intervening sequences of S. cerevisiae genes (30). This latter sequence has been postulated to be required for RNA splicing in S.…”

Section: Resultsmentioning

confidence: 90%

“…The intervening sequences were short (ranging from 55 to 75 bp) and were all located within protein-encoding sequences. Short intervening sequences have also been found in genes of other filamentous ascomycetes, namely, the cellobiohydrolase gene of Trichoderma reesei (54) and the glutamate dehydrogenase gene (26) and histone H3 and H4 genes (67) of Neurospora crassa. In contrast, the trp-J gene of N. crassa has been shown to lack intervening sequences (50 5' end of the glucoamylase gene was hybridized to total poly(A)+ mRNA from starchgrown cells, and S1 nuclease-resistant products were analyzed by gel electrophoresis under denatured conditions (49).…”

Section: Resultsmentioning

confidence: 99%

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Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

Nunberg¹,

Meade²,

Cole³

et al. 1984

Mol. Cell. Biol.

160

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The filamentous ascomycete Aspergillus awamori secretes large amounts of glucoamylase upon growth in medium containing starch, glucose, or a variety of hexose sugars and sugar polymers. We examined the mechanism of this carbon source-dependent regulation of glucoamylase accumulation and found a several hundredfold increase in glucoamylase mRNA in cells grown on an inducing substrate, starch, relative to cells grown on a noninducing substrate, xylose. We postulate that induction of glucoamylase synthesis is regulated transcriptionally. Comparing total mRNA from cells grown on starch and xylose, we were able to identify an inducible 2.3-kilobase mRNA-encoding glucoamylase. The glucoamylase mRNA was purified and used to identify a molecularly cloned 3.4-kilobase EcoRI fragment containing the A. awamori glucoamylase gene. Comparison of the nucleotide sequence of the 3.4-kilobase EcoRI fragment with that of the glucoamylase I mRNA (as determined from molecularly cloned cDNA) revealed the existence of four intervening sequences within the glucoamylase gene. The 5' end of the glucoamylase mRNA was mapped to several locations within a region -52 to -73 nucleotides from the translational start. Sequence and structural features of the glucoamylase gene of the filamentous ascomycete A. awamori were examined and compared with those reported in genes of other eucaryotes.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

Nunberg¹,

Meade²,

Cole³

et al. 1984

Mol. Cell. Biol.

160

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“…That presumed d(ATG) start codon is preceeded by a short sequence d(CACA) similar to the start codon of other Neurospora crassa mRNAs [13,15,[22][23][24][25]. The start of the mRNA has not yet been determined.…”

Section: Isolation Of Cloned Cdma and Genomic Dnamentioning

confidence: 99%

The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing

1985

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The primary structure of the iron-sulfur subunit of ubiquinol -cytochrome c reductase from Neurosporu mitochondria was determined by cDNA and genomic DNA sequencing. A first cDNA was identified from a cDNA bank cloned in Escherichia coli by hybridization selection of mRNA, cell-free protein synthesis and immunoadsorption. Further cDNA and geonomic DNA were identified by colony filter hybridization. The N-terminal sequence of the mature protein was determined by automated Edman degradation. From the sequence a molecular mass of 24749 Da results for the precursor protein and of 21 556 Da for the mature protein. The presequence consists of 32 amino acids with four arginines as the only charged residues. The mature protein consists of 199 amino acids. It is characterized by a small N-terminal hydrophilic part of 29 residues, a hydrophobic stretch of 25 residues and a large C-terminal hydrophilic domain of 145 residues. The only four cysteines of the protein, which are assumed to bind the 2Fe-2S cluster, are located in a moderate hydrophobic region of this large domain. Cysteines 3 and 4 are unusually arranged in that they are separated by only one proline. From sequence data the arrangement of the subunit in the membrane is deduced.Ubiquinol -cytochrome c reductase (cytochrome reductase) is a proton-translocating enzyme complex of the oxidative phosphorylation system in mitochondria. The enzyme isolated from Neurosporu consists of the cytochromes b and cl, an iron-sulfur subunit (Rieske 2Fe-2S protein, [l]) and six subunits without redox centers [2, 31.The three-dimensional structure of cytochrome reductase, the arrangement of the structure in the mitochondrial inner membrane and the topography of most of the subunits within the structure have been studied by electron microscopy of twodimensional crystals, neutron diffraction of enzyme/detergent preparations and biochemical characterization of isolated subunits [3 -71. With regard to the iron-sulfur subunit the studies showed that the subunit extends from the membrane with a large domain which carries the 2Fe-2S cluster into the intermembrane space of mitochondria and is anchored to the bilayer only by a small protein part.The import of the cytoplasmically synthesized subunits of the Neurospora cytochrome reductase (all except cytochrome b, which is a mitochondrial coded protein) into mitochondria has been studied recently [8]. The subunits, except the 14000-Da subunit, are synthesized as larger precursors and proteolytically processed during or after their import into mitochondria. Similar results were obtained for the yeast enzyme [9 -121.In this article we report on the primary structure of the iron-sulfur subunit of Neurospora cytochrome reductase in its

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“…The incomplete unit structure and dispersed organization of histone genes in genomes of plants imply that highly diverged flanking regions can exist near histone genes. In the H3 gene, although introns are found only in the H3.3 variant of plants (Chaboute et al, 1992), animals (Akhmanova et al, 1995;, and some lower eukaryotes (Muller and Schmitt, 1988;Woudt et al, 1983;Ehinger et al,1990), the H3 genes of liverwort contain introns of various types (Fig. 3).…”

Section: Discussionmentioning

confidence: 99%

Nonconcerted evolution of histone 3 genes in a liverwort, Conocephalum conicum

Kim

Yamazaki

2004

Genes Genet. Syst.

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To estimate the extent of genetic variation at the DNA level, the histone 3 (H3) genes were sequenced from single individual each from the three cryptic species recognized based on allozyme analyses, YFS, J and T types of Conocephalum conicum and two closely related species, C. japonicum and Marchantia polymorpha . Although the H3 genes are known to be highly conserved, the nucleotide diversities were 0.128, 0.109, 0.108, 0.049 and 0.034. These values are 30 to 100 times higher than that in Drosophila melanogaster (0.001). Besides, there were considerable differences in the position, length and number of introns among the loci of H3 genes. The observed high level of nucleotide diversities was explained by the fixation of many random mutations, and non-concerted evolution that resulted from low rates of unequal crossing-over and gene conversion probably due to the dispersed structure of H3 genes on genome in this species. The non-concerted evolutionary pattern was established by the analysis of phylogenetic tree and divergence rates. This study confirmed previous results suggesting that natural populations of liverwort maintains high extent of variation at DNA level.

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The genes coding for histone H3 and H4 inNeurospora crassaare unique and contain intervening sequences

Cited by 138 publications

References 31 publications

Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing

Nonconcerted evolution of histone 3 genes in a liverwort, Conocephalum conicum

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