The generation and characterization of an ovary-selective cDNA library

Tanaka, Mamoru; Hennebold, Jon D.; Miyakoshi, Kei; Teranishi, Takahide; Ueno, Kazunori; Adashi, Eli Y.

doi:10.1016/s0303-7207(03)00064-9

Cited by 12 publications

(10 citation statements)

References 12 publications

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“…Thirty clones were expressed at a same or higher level in the 48-h PMSG (preovulatory) ovarian mRNA relative to the post-hCG (ovulatory) mRNA, giving a false-positive rate of 41%. This rate is within the accepted range of the reported false-positive rate for the SSH technique, as it varies very much depending on experimental circumstances (Lee et al 2000, Tanaka et al 2003, Fayad et al 2004.…”

Section: Discussionsupporting

confidence: 74%

“…Other advantages are the normalization of the representation of high and low abundance transcripts, and the elimination of the physical subtraction step in the isolation of target cDNAs (Lee et al 2000, Levesque et al 2003, Fayad et al 2004, Rebrikov et al 2004. Moreover, the successful use of this PCR-based method has previously been reported in the context of constructing testis-specific library (Diatchenko et al 1996) and by our laboratory in constructing an ovary-specific library (Tanaka et al 2003). The discovery of new ovulatory genes in this study confirms the potential of this technique.…”

Section: Discussionmentioning

confidence: 96%

“…The panel shown reflects a representative experiment from a total of three independent experiments. incomplete representation of the total cDNA repertoire (den Hollander et al 1999, Tanaka et al 2003.…”

Section: Discussionmentioning

confidence: 99%

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Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library

Hourvitz

Gershon

Hennebold³

et al. 2006

Journal of Endocrinology

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Ovulation-selective/specific genes, that is, genes preferentially or exclusively expressed during the ovulatory process, have been the subject of growing interest. We report herein studies on the use of suppression subtractive hybridization (SSH) to construct a 'forward' ovulationselective/specific cDNA library. In toto, 485 clones were sequenced and analyzed for homology to known genes with the basic local alignment tool (BLAST). Of those, 252 were determined to be nonredundant. Of these 252 nonredundant clones, 98 were analyzed by probing mouse preovulatory and postovulatory ovarian cDNA. Twentyfive clones (26%) failed to show any signal, and 43 cDNAs tested thus far display a true ovulation-selective/specific expression pattern. In this communication, we focus on one such ovulation-selective gene, the fatty acid elongase 1 (FAE-1) homolog, found to be localized to the inner periantral granulosa and to the cumulus granulosa cells of antral follicles. The FAE-1 gene is a -ketoacyl-CoA synthase belonging to the fatty acid elongase (ELO) family, which catalyzes the initial step of very long-chain fatty acid synthesis. All in all, the present study accomplished systematic identification of those hormonally regulated genes that are expressed in the ovary in an ovulation-selective/specific manner. These ovulationselective/specific genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for both the promotion of fertility and its control.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 96%

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Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library

Hourvitz

Gershon

Hennebold³

et al. 2006

Journal of Endocrinology

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“…However, this transcript variant, with orthologues in the sheep, pig and human EST libraries, consists only of exons 1-5 of the aromatase gene, and thus may have no further function following translation (Levallet et al 1998). A new gene with so far unknown functions, OSAP, identified in mouse preovulatory follicles, may be a marker of differentiated follicle development, specifically of increased E synthesis, with gene expression responding to gonadotrophic stimulation (Hennebold et al 2000, Tanaka et al 2003. We propose, therefore, that the new genes identified by the SAGE and real-time PCR analyses, which show enhanced expression in the DF, may regulate proliferation, prevention of apoptosis or DNA damage, RNA synthesis and unknown processes associated with enhanced steroidogenesis in granulosa cells of the DFat the onset of its LH-dependent development.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes

Mihm¹,

Baker²,

Fleming³

et al. 2008

Reproduction

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This study was designed to identify genes that regulate the transition from FSH-to LH-dependent development in the bovine dominant follicle (DF). Serial analysis of gene expression (SAGE) was used to compare the transcriptome of granulosa cells isolated from the most oestrogenic growing cohort follicle (COH), the newly selected DF and its largest subordinate follicle (SF) which is destined for atresia. Follicle diameter, follicular fluid oestradiol (E) and E:progesterone ratio confirmed follicle identity. Results show that there are 93 transcript species differentially expressed in DF granulosa cells, but only 8 of these encode proteins known to be involved in DF development. Most characterised transcripts upregulated in the DF are from tissue development genes that regulate cell differentiation, proliferation, apoptosis, signalling and tissue remodelling. Semiquantitative real-time PCR analysis confirmed seven genes with upregulated (P%0.05) mRNA expression in DF compared with both COH and SF granulosa cells. Thus, the new genes identified by SAGE and real-time PCR, which show enhanced mRNA expression in the DF, may regulate proliferation (cyclin D2; CCND2), prevention of apoptosis or DNA damage (growth arrest and DNA damage-inducible, b; GADD45B), RNA synthesis (splicing factor, arginine/serine rich 9; SFRS9) and unknown processes associated with enhanced steroidogenesis (ovary-specific acidic protein; DQ004742) in granulosa cells of DF at the onset of LH-dependent development. Further studies are required to show whether the expression of identified genes is dysregulated when abnormalities occur during DF selection or subsequent development.

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“…FSH drives the proliferation, growth and differentiation of granulosa cells, characterized by: increased vascularization of the theca interna layer of cells peripheral to the basal lamina, formation of a fluid-filled antrum within the maturing follicle, and development of two "classes" of granulosa cells with distinct polarities and gene expression (the cumulus granulosa cells that surround the oocyte and mural granulosa cells that are peripheral to the antrum and line the basal lamina). These physiological responses to FSH are accomplished by the activation of more than 100 different target genes in granulosa cells [1][2][3], as depicted in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

FSH signaling pathways in immature granulosa cells that regulate target gene expression: Branching out from protein kinase A

Hunzicker-Dunn

Maizels

2006

Cellular Signalling

325

240

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Follicle-stimulating hormone (FSH) is necessary and sufficient to induce maturation of ovarian follicles to a mature, preovulatory phenotype in the intact animal, resulting in the generation of mature eggs and production of estrogen. FSH accomplishes these actions by inducing a complex pattern of gene expression in target granulosa cells that is regulated by input from many different signaling cascades, including those for the extracellular regulated kinases (ERKs), p38 mitogen-activated protein kinases (MAPKs), and phosphatidylinositol-3 kinase (PI3K). The upstream kinase that appears to be responsible for initiating all of the signaling that regulates gene expression in these epithelial cells is protein kinase A (PKA). PKA not only signals to directly phosphorylate transcription factors like cAMP response element binding protein and to promote chromatin remodeling by phosphorylating histone H3, this versatile kinase also enhances the activity of the p38 MAPK, ERK, and PI3K pathways. Additionally, accumulating evidence suggests that activation of a single signaling cascade downstream of PKA is not sufficient to activate target gene expression. Rather, cross-talk between and among signaling cascades is required. We will review the signaling cascades activated by FSH in granulosa cells and how these cascades contribute to the regulation of select target gene expression. KeywordsFollicle-stimulating hormone; Mitogen-activated protein kinase; Female reproduction; Hypoxiainduced factor 1; Histone H3; Protein kinase A Abbreviations AKAP, A kinase anchoring protein; Aromatase, P450 aromatase; CBP, CREB binding protein; ChIP, chromatin immunoprecipitation assay; CREB, cAMP response element binding protein; EGF, epidermal growth factor; Egr-1, early growth response protein-1; ERK, extracellular regulated kinase; Epac, exchange proteins activated by cAMP; FSH, follicle-stimulating hormone; GIOT-1, gonadotropin-inducible ovarian transcription factor-1; GPCR, G-protein-coupled receptor; HIF-1, hypoxia-induced factor-1; IGF, insulin-like growth factor; LH, luteinizing hormone; LRH-1, liver receptor homolog-1; MAP2D, microtubule-associated protein 2D; MAPK, mitogen-activated protein kinase; MEK, mitogen-and extracellular-regulated kinase kinase; MK, MAPK-activated protein kinases; MNK, MAPK-interacting kinase; mTOR, mammalian target of rapamycin; p70S6K, p70 ribosomal S6 kinase; PDE, phosphodiesterase; PI3K, phosphatidylinositol 3-kinase; PKA, protein kinase A; PKC, protein kinase C; PKI, PKA inhibitor peptide; PTP, protein tyrosine phosphatase; R, PKA regulatory subunits; RSK, p90 ribosomal S6 protein kinase; SCC, P450 cholesterol side chain cleavage; SF-1, steroidogenic factor-1; SGK, serum glucocorticoid kinase; Sp1/Sp3, specific

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The generation and characterization of an ovary-selective cDNA library

Cited by 12 publications

References 12 publications

Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library

Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library

Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes

FSH signaling pathways in immature granulosa cells that regulate target gene expression: Branching out from protein kinase A

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