About a decade ago a study documented that conservatives have stronger physiological responses to threatening stimuli than liberals. This work launched a paradigm aimed at uncovering the biological roots of ideology. Despite wide-ranging scientific and popular impact, independent laboratories have not replicated the study. We conducted a preregistered direct replication (N=202) and conceptual replications in the United States (N=352) and the Netherlands (N=81). Our analyses do not support the conclusions of the original study, nor do we find evidence for broader claims regarding the effect of disgust and the existence of a physiological trait. Rather than studying unconscious responses as the "real" predispositions, alignment between conscious and unconscious responses promise deeper insights in the emotional roots of ideology. Main In a study published by Science, Oxley et al. 1 demonstrate that individuals who express conservative political attitudes (e.g., a preference for tradition, clear group boundaries, and hierarchies) have stronger physiological reactions to threatening stimuli than those who express liberal views. One of the authors' explanations for this association is that "political attitudes and varying physiological responses to threat may both derive from neural activity patterns" (p.1669), 1 suggesting a biological basis for the robust, positive correlation between political conservatism and self-reported measures of sensitivity to uncertainty and threat 2-5. Research by others, including the Oxley, et al. research team, demonstrates that conservatives also have stronger physiological responses to disgusting stimuli than liberals do 6, 7. These findings motivated members of the Oxley, et al. research team to further elaborate that conservatism is linked to a more expansive form of "negativity bias" at a physiological level 9. This research helped usher in a new paradigm in psychology and political science that offers a provocative perspective on the biological roots of the centuries-old liberal/conservative divide 8. It implies that psychological traits, such as "threat sensitivity", "disgust sensitivity", and "negativity bias" can be captured in a straightforward and unobtrusive way with physiological reactions, and that these "physiological traits" have a direct and unmediated effect on