The Genera Staphylococcus and Macrococcus

Götz, Friedrich; Bannerman, Tammy L.; Schleifer, Karl‐Heinz

doi:10.1007/0-387-30744-3_1

Cited by 158 publications

(143 citation statements)

References 368 publications

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“…The species epithet of Staphylococcus aureus reflects the color of its colonies (L. aureus: golden) and distinguish this species from Staphylococcus epidermidis (formerly Staphylococcus albus) (1). The orange pigmentation of S. aureus had been used as a species character until colorless strains were observed (2).…”

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confidence: 99%

Structure and Biosynthesis of Staphyloxanthin from Staphylococcus aureus

Pelz

Wieland

Putzbach

et al. 2005

Journal of Biological Chemistry

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confidence: 99%

Structure and Biosynthesis of Staphyloxanthin from Staphylococcus aureus

Pelz

Wieland

Putzbach

et al. 2005

Journal of Biological Chemistry

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300

280

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“…It is known that S. warneri and S. hominis species are not pathogenic [36] and we showed that this was also likely to be true 3 million years ago, as no virulence genes were identified in either of these ancient Staphylococcus strains.…”

Section: Discussionmentioning

confidence: 74%

“…Staphylococcus species are divergent in their pathogenicity [36], and within species, patterns of virulence genes can be drastically different [44]. It is known that S. warneri and S. hominis species are not pathogenic [36] and we showed that this was also likely to be true 3 million years ago, as no virulence genes were identified in either of these ancient Staphylococcus strains.…”

Section: Discussionmentioning

confidence: 74%

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Ancient permafrost staphylococci carry antibiotic resistance genes

Kashuba

Dmitriev

Kamal

et al. 2017

Microbial Ecology in Health and Disease

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Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years.Objective: To cultivate bacterial strains from permafrost.Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40–60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences.Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome.Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance.

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“…In S. aureus 85% of Glucose is catabolised through EMP pathway and the very first step of the formation of G-6-P is carried out in two pathways one through phosphotransferase (PTS) which usually functions under low glucose concentration and the second PTS independent system catalysed by glucose permease (glk U) and glucokinase (glk A) which functions mostly under high glucose concentration [2]. This G-6-P plays critical role in the pathogen for energy generation in catabolic reactions for the synthesis of all the intermediates for its very survival [3].…”

Section: Introductionmentioning

confidence: 99%

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Abstract:Glucose-6-phosphate (G-6-P) formation in Staphylococcus aureus is catalysed by glucokinase (glkA) gene under high glucose concentration leading to upregulation of various pathogenic factors; therefore the present study is aimed in the cloning and characterization of glk A gene from S. aureus ATCC12600. The glk A gene was cloned in the Sma I site of pQE 30, sequenced (Accession number: JN645812) and expressed in E. coli DH5α. The recombinant glk A expressed from the resultant glk A 1 clone was purified using nickel metal chelate chromatography, the pure enzyme gave single band in SDS-PAGE with molecular weight of 33kDa. The rglk A showed very high affinity to glucose K m 5.1±0.06mM with Hill coefficient of 1.66±0.032mM. Analysis of glucokinase sequence of S. aureus showed presence of typical ATP binding site and ROK motif CNCGRSGCIE. Sequentially and phylogenetically S. aureus glk A exhibited low identity with other bacterial glk A and 21% homology with human glucokinase (GCK). Functionally, S. aureus glk A showed higher rate of G-6-P formation compared to human GCK which may have profound role in the pathogenesis.Keywords: glk A, pQE 30, ROK, K m . Background:Staphylococcus aureus is an important nosocomial and community-acquired pathogen and its infections are known to be a leading cause of bacteremia and are associated with severe morbidity and mortality in hospitalized infections [1]. In S. aureus 85% of Glucose is catabolised through EMP pathway and the very first step of the formation of G-6-P is carried out in two pathways one through phosphotransferase (PTS) which usually functions under low glucose concentration and the second PTS independent system catalysed by glucose permease (glk U) and glucokinase (glk A) which functions mostly under high glucose concentration [2]. This G-6-P plays critical role in the pathogen for energy generation in catabolic reactions for the synthesis of all the intermediates for its very survival [3].

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The Genera Staphylococcus and Macrococcus

Cited by 158 publications

References 368 publications

Structure and Biosynthesis of Staphyloxanthin from Staphylococcus aureus

Structure and Biosynthesis of Staphyloxanthin from Staphylococcus aureus

Ancient permafrost staphylococci carry antibiotic resistance genes

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