The Gene YALI0E20207g from Yarrowia lipolytica Encodes an N-Acetylglucosamine Kinase Implicated in the Regulated Expression of the Genes from the N-Acetylglucosamine Assimilatory Pathway

Flores, Carmen‐Lisset; Gancedo, Carlos

doi:10.1371/journal.pone.0122135

Cited by 8 publications

(21 citation statements)

References 84 publications

(107 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also tested whether growth on GlcNAc as a sole carbon source would lead to hyphae formation. However, no hyphae formation was observed under these conditions, in line with a previous study [22]. Since our wild type strain did not show a high filamentation rate in the tested liquid media, we also decided to investigate if hyphae formation would be observed on solid media.…”

Section: Comparison Of Mutations Reported To Abolish Hyphae Formationsupporting

confidence: 77%

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Konzock¹,

Norbeck²

2020

PLoS ONE

View full text Add to dashboard Cite

There is a need for development of sustainable production processes for production of fats/ oils and lipid derived chemicals. The dimorphic oleaginous yeast Yarrowia lipolytica is a promising organism for conversion of biomass hydrolysate to lipids, but in many such processes hyphae formation will be problematic. We have therefore constructed and compared the performance of strains carrying deletions in several published gene targets suggested to abolish hyphae formation (MHY1, HOY1 and CLA4). The MHY1-deletion was the only of the tested strains which did not exhibit hyphae formation under any of the conditions tested. The MHY1-deletion also had a weak positive effect on lipid accumulation without affecting the total fatty acid composition, irrespective of the nitrogen source used. MHY1 has been suggested to constitute a functional homolog of the stress responsive transcription factors MSN2/4 in Saccharomyces cerevisiae, the deletion of which are highly stress sensitive. However, the deletion of MHY1 displayed only minor difference on survival of a range of acute or long term stress and starvation conditions. We conclude that the deletion of MHY1 in Y.lipolytica is a reliable way of abolishing hyphae formation with few detectable negative side effects regarding growth, stress tolerance and lipid accumulation and composition.

show abstract

Section: Comparison Of Mutations Reported To Abolish Hyphae Formationsupporting

confidence: 77%

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Konzock¹,

Norbeck²

2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…In dimorphic yeasts, such as C. albicans and Y. lipolytica , GlcNAc is transported across the cell membrane and phosphorylated to GlcNAc6P by N -acetylglucosamine kinase (EC 2.7.1.59). Then, GlcNAc6P is deacetylated by N -acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) to form GlcN-6-phosphate (GlcN6P), followed by deamination by glucosamine-6-phosphate deaminase (EC 3.5.99.6) to produce F6P [ 7 , 11 ]. On the other hand, some bacteria, such as Escherichia coli , Bacillus cadaveris , Streptococcus faecalis , and Vibrio cholerae, have N -acetylglucosamine deacetylase (EC 3.5.1.33) activity and convert GlcNAc into GlcN [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Chitinolytic organisms, such as marine and soil bacteria and fungi, degrade chitin into GlcNAc and then utilize it as their principal source of carbon and nitrogen [ 2 – 4 ]. On the other hand, many yeast species including the most widely used industrial yeast, Saccharomyces cerevisiae , have lost their ability to utilize GlcNAc [ 5 ] except for some dimorphic yeast species that separated early from the common yeast evolutionary trunk [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…In dimorphic yeasts such as Candida albicans and Yarrowia lipolytica, GlcNAc is known to be a potent inducer of morphological transition [ 8 ]. Numerous studies focused on the GlcNAc metabolic pathway in these yeasts and the effect of GlcNAc on their morphogenesis have been reported [ 7 , 9 – 11 ]. However, few studies have evaluated the metabolic response to GlcNAc in these yeast strains [ 12 ], and no comprehensive analysis has been published concerning the influence of GlcNAc on the metabolism of nitrogen-containing compounds such as amino acids, purines, and pyrimidines in yeast cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Widespread effect of N-acetyl-d-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

et al. 2018

View full text Add to dashboard Cite

BackgroundFollowing cellulose, chitin is the most abundant renewable resource and is composed of the monomeric amino sugar N-acetyl-d-glucosamine (GlcNAc). Although many yeasts, including Saccharomyces cerevisiae, have lost their ability to utilize GlcNAc, some yeasts are able to use GlcNAc as a carbon source. However, our understanding of the effects of GlcNAc on the intracellular metabolism of nitrogen-containing compounds in these yeast species is limited.ResultsIn the present study, we quantitatively investigated the metabolic responses to GlcNAc in the GlcNAc-assimilating yeast Scheffersomyces stipitis (formerly known as Pichia stipitis) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The comprehensive analysis of the metabolites extracted from S. stipitis cells grown in glucose, xylose, or GlcNAc revealed increased intracellular accumulation of a wide range of nitrogen-containing compounds during GlcNAc assimilation in this yeast. The levels of aromatic, branched-chain, and sulfur-containing amino acids and adenine, guanine, and cytosine nucleotides were the highest in GlcNAc-grown cells.ConclusionsThe CE-TOFMS analysis revealed a positive effect for GlcNAc on the intracellular concentration of a wide range of nitrogen-containing compounds. The metabolomic data gathered in this study will be useful for designing effective genetic engineering strategies to develop novel S. stipitis strains for the production of valuable nitrogen-containing compounds from GlcNAc.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0998-4) contains supplementary material, which is available to authorized users.

show abstract

“…Wild-type Yarrowia lipolytica strains can only utilize biomass-derived sugars such as glucose, fructose and mannose as substrates (Michely et al, 2013;Flores and Gancedo, 2015). They are unable to use disaccharides or sugar polymers.…”

Section: Introductionmentioning

confidence: 99%

Transforming sugars into fat - lipid biosynthesis using different sugars inYarrowia lipolytica

Hapeta

Rakicka

Dulermo

et al. 2017

Yeast

View full text Add to dashboard Cite

In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus. The second strain, Y. lipolytica Y4779, was derived from the Polish A-101 strain. It had inhibited β-oxidation (∆mfe2) and overexpressed GPD1, DGA1, HXK1, YHT3, SUC2 and INU1. In the first experiment, strain YLZ150 was batch-cultured in media containing different hexoses; the highest values for lipid concentration and yield of lipids from the substrate were obtained using fructose (20.3 g dm and 0.14 g g , respectively). In the second experiment, we grew the two strains in fed-batch cultures to examine lipid biosynthesis from inulin (a fructose polymer). For Y4779, the lipid concentration was 10.3 g dm and the yield of lipids from substrate was 0.07 g g ; in contrast, for YLZ150, these values were 24 g dm and 0.16 g g , respectively. The YLZ150 strain is thus able to efficiently exploit glucose, fructose, galactose, sucrose and inulin for lipid biosynthesis. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

The Gene YALI0E20207g from Yarrowia lipolytica Encodes an N-Acetylglucosamine Kinase Implicated in the Regulated Expression of the Genes from the N-Acetylglucosamine Assimilatory Pathway

Cited by 8 publications

References 84 publications

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance

Widespread effect of N-acetyl-d-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

Transforming sugars into fat - lipid biosynthesis using different sugars inYarrowia lipolytica

Contact Info

Product

Resources

About