The gene ontology and electro localization of ovine skin derived EST-SSR markers

Abstract

In order to study the potential gene function of ovine EST-SSR markers, nine original EST of Ovine Skin Derived polymorphic EST-SSR loci, which were developed in an early study by our lab, were ontology annotated and Electro localized. The results revealed that the original ESTs of the six loci had high homology with known genes and three of them probably played an important role in wool traits. Compared with its cDNA library, 8 loci were located on chromosomes of cattle. The homology of chromosomes between cattle and sheep was estimated based on the similarity coefficients calculated by positioning markers. Additionally, NJ clustering tree was establishedto serve for electro localization of ovine EST-SSR markers. Finally, 8 EST-SSR markers were successfully positioned on ovine chromosomes. The results from this study not only provide references for further studies on genetic mapping, in silico cloning of key genes for wool traits, but also are helpful to the researchs of chromosome evolution in animal.

Development and Application of Est-Ssr Markers in Quails

“…The average number of effective alleles were 2.5117, 2.9151, and 2.5323, respectively. The average number of alleles and effective alleles of quail EST-SSR were higher than those of shrimp, developed by Zhang et al (2010), who obtained 2.4000±0.5477 alleles and 2.0600±0.3287effective alleles, but lower than that of sheep (3.4266), as determined by Wang et al (2011a). In the present study, except for P6, which was lowly polymorphic, the other five EST-SSR markers were moderately or highly polymorphic in the three quail populations, suggesting that the six newly developed EST-SSR markers may be used as a new molecular marker and are suitable to analyze the genetic diversity of quail populations.…”

“…SSRs developed from ESTs has been reported in many plants, such as grapes (Pen et al, 2005), wheat (Eujayi et al, 2002), and barley (Thie et al, 2003), also in animals, such as zebra fish (Serapion et al, 2004), prawns (Perez et al, 2005,Zhang et al, 2010, catfish (Ju et al, 2005), sheep (Yan et al, 2007,Wang et al, 2010,Wang et al, 2011a,Wang et al, 2011b,Zhang et al, 2014, goat (Feng et al, 2008,Zhao et al, 2009, and silkworms (Bombyx mori; Mi et al, 2011). EST, as a new developed molecular marker, reflects the gene encoding part.…”