The gene expression profile of psoralen plus UVA-induced premature senescence in skin fibroblasts resembles a combined DNA-damage and stress-induced cellular senescence response phenotype

Borlon, Céline; Debacq‐Chainiaux, Florence; Hinrichs, Christina; Scharffetter‐­Kochanek, Karin; Toussaint, Olivier; Wlaschek, Meinhard

doi:10.1016/j.exger.2007.04.009

Cited by 28 publications

(27 citation statements)

References 60 publications

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“…More than 40 verifications with real-time RT PCR of the relative gene expression level obtained with this microarray have already been done in several senescence-related models (Borlon et al 2007a;de Magalhaes et al 2004;Debacq-Chainiaux et al 2005;Zdanov et al 2006). Nevertheless, further verifications were done in the studies.…”

Section: Validation Of Relative Gene Expression By Real Time Rt-pcrmentioning

confidence: 99%

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Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

et al. 2008

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It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-β1 was up-regulated in the dermis of old donors as well as the TGF-β1 -regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione STransferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging.

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Section: Validation Of Relative Gene Expression By Real Time Rt-pcrmentioning

confidence: 99%

“…UV irradiations inhibit collagen synthesis (Brenneisen et al 2002;Rittie and Fisher 2002). Both collagens COLIAI and COL3A1 were already found as down-regulated after psoralen plus UVAinduced premature senescence of skin fibroblasts (Borlon et al 2007a).…”

Section: Low Density Cdna Microarray Experiments and Data Analysismentioning

confidence: 99%

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

et al. 2008

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“…The phenomenon is an aspect of every living organism. Physiological ageing is associated with a progressive decline in cellular functions, an increased risk of major diseases and alteration in gene expression, protein processing and metabolic processes [1]. As evidence indicated in previous studies that accumulation of cellular senescence in some organs or tissues contribute to ageing or several age-related diseases [2,3].…”

Section: Introductionmentioning

confidence: 93%

“…Many different factors have been shown to cause ageing, including lifestyle-related habits, environmental exposures, Reactive Oxygen Species (ROS), and stress. Among the negative factors contributing to aging, the most studied of them is stress [1]. Chronic stress has been widely associated with brain ageing, alterations in neuronal morphology, function and neurogenesis [4].…”

Section: Introductionmentioning

confidence: 99%

“…The levels of miR-709 and miR-669c increase at 18 months, and reach maximal levels at 33 months [7]. In a recent study, ageing in Leydig cells of testis has drawn great attention since it influences the spermatogenesis that is a cellular process to produce mature male germ cells [1]. Interestingly, the expression of 19 miRNAs has been shown to be significantly different between mature and immature mouse testis [1].…”

Section: Introductionmentioning

confidence: 99%

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Alternations in miRNA Expression in Chronic Stress-Induced Ageing of Leydig Cells

Zhou¹,

Chen²,

Wen³

et al. 2017

Andrology

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Purpose: Chronic stress represents a critical influence on ageing of Leydig cells in testis. We aimed to investigate expression changes of microRNA (miRNA) associated with stress-induced ageing in Leydig cells. Methods:Brown Norway rats were separated into three groups, young control group, stress group and age group. Physiological changes, including serum corticosterone and testosterone levels, 11β-HSD (11β-hydroxysteroiddehydrogenase) activity, GSH/GSSG (glutathione/glutathione disulfide) ratio and DNA damage after chronic stress treatment were tested by radioimmunoassay and Immunohistochemistry. Differentially expressed miRNAs in Leydig cells between young control group and stress group were identified by miRNA microarray analysis, followed by target prediction of miRNAs. Functional annotation of predicted targets of miRNA was carried out using GeneSpring software and miRNA-target gene interaction network was built by String software.Results: Under chronic stress, increased serum corticosterone levels and reduced testosterone levels were observed. Meanwhile, decline of 11β-HSD activity and GSH/GSSG ratio, increase of DNA damage were also shown in stress group. After performed miRNA microarray analysis, three differentially expressed miRNAs were screened out, including rno-miR-31a-5p, rno-miR-192-5p and rno-miR-191a-3p. Targets of them were mainly involved in apoptosis, cell cycle and transport. In miRNA-target gene interaction network, targets of miR-31a-5p (Tp53, Ywhae and Ndel1), and targets of miR-192-5p (Cdk2, Rac2, Stk3) were with higher degree. Conclusion:These data suggest a central role of miRNA-regulated gene expression in response to chronic stress, especially dys-regulation of rno-miR-31a-5p and rno-miR-192-5p play a vital role in ageing of Leydig cells in testis.

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Protein Oxidation and Aging: Different Model Systems and Affecting Factors

Grune¹,

Karademir²,

Jung³

2012

Protein Oxidation and Aging

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The gene expression profile of psoralen plus UVA-induced premature senescence in skin fibroblasts resembles a combined DNA-damage and stress-induced cellular senescence response phenotype

Cited by 28 publications

References 60 publications

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Alternations in miRNA Expression in Chronic Stress-Induced Ageing of Leydig Cells

Protein Oxidation and Aging: Different Model Systems and Affecting Factors

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