The Gene Encoding the Universal Stress Protein AtUSP is Regulated by Phytohormones and Involved in Seed Germination of Arabidopsis thaliana

Gorshkova, D. S.; Getman, Irina A.; Воронков, А. С.; Chizhova, S. I.; Кузнецов, Вл. В.; Pojidaeva, E. S.

doi:10.1134/s1607672918020151

Cited by 16 publications

(10 citation statements)

References 14 publications

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“…It was found that AtUSP functions as a molecular chaperone under oxidative, heat, and cold-shock conditions (Jung et al 2015;Melencion et al 2017). Gorshkova et al (2018) indicated that AtUSP is regulated by phytohormones and is involved in seed germination of Arabidopsis. The role of USPs in maintaining black poplar seed longevity could be associated with chaperon activity, but that should be verified in future experiments.…”

Section: Proteins Involved In Stress Responsesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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& Key message Adequate storability of black poplar (Populus nigra L.) seeds at temperatures below 0°C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining longterm stability of dehydrated tissue. & Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. & Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. & Methods Dried seeds (7.1% MC) were stored at 3°C, − 3°C, − 20°C, and − 196°C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. & Results A germination test showed that seeds remained viable at − 3, − 20, and − 196°C. Storage at 3°C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. & Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.

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Section: Proteins Involved In Stress Responsesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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“…In Arabidopsis , overexpression of USP has been shown to enhance resistance to oxidative stress and fungal pathogen attack [9,12]. In addition, the USP promoter region is reportedly regulated by seed germination‐related phytohormones and abiotic stress responses [13,14]. The closest homolog of USP in Arabidopsis , Hypoxia Responsive Universal Stress Protein 1 ( HRU1 ), mediates reactive oxygen species (ROS) homeostasis signaling under anoxic conditions by sensing the level of oxygen [15].…”

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confidence: 99%

Universal Stress Protein regulates the circadian rhythm of central oscillator genes in Arabidopsis

et al. 2022

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Environmental stresses restrict plant growth and development and decrease crop yield. The circadian clock is associated with the ability of a plant to adapt to daily environmental fluctuations and the production and consumption of energy. Here, we investigated the role of Arabidopsis Universal Stress Protein (USP; At3g53990) in the circadian regulation of nuclear clock genes. The Arabidopsis usp knockout mutant line exhibited critically diminished circadian amplitude of the central oscillator CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) but enhanced the amplitude of TIMING OF CAB EXPRESSION 1 (TOC1). However, the expression of USP under the control of its own promoter restored the circadian timing of both genes, suggesting that USP regulates the circadian rhythm of Arabidopsis central clock genes, CCA1 and TOC1.

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“…GRUSP protein (Germination-Related Universal Stress Protein) encoded by At3g58450 gene A. thaliana [ 12 , 13 ] is a potential member in pathways controlling the balance between ABA and GA throughout different growth stages. At3g58450 expression significantly increases in Col-0 seedlings treated with ABA, and impairment of its transcription causes ABA hypersensitivity of transgenic seeds grusp-115 [ 12 ].…”

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confidence: 99%

“…In this study, we used A. thaliana (L.) Heynh ecotype Columbia wild type (Col-0) and GABI_kat 115C08 ( grusp-115 ) homozygous transgenic line [ 12 ]. Plants were grown in a chamber with a controlled environment at 16-h photoperiod, light intensity of 100 μmol m –2 s –1 and temperature +21°C.…”

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confidence: 99%

“…The aim of this study is to analyze for the first time the relationship of a late-flowering phenotype of grusp-115 transgenic line, characterized by suppressed expression of At3g58450 due to T-DNA insertion in the 3'-UTR, with the endogenous gibberellins content, as well as with the expression of genes that control the transition to reproductive development. In this study, we used A. thaliana (L.) Heynh ecotype Columbia wild type (Col-0) and GABI_kat 115C08 (grusp-115) homozygous transgenic line [12]. Plants were grown in a chamber with a controlled environment at 16-h photoperiod, light intensity of 100 μmol m -2 s -1 and temperature +21°C.…”

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confidence: 99%

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GRUSP, an Universal Stress Protein, Is Involved in Gibberellin-dependent Induction of Flowering in Arabidopsis thaliana

Gorshkova

Getman

Сергеева

et al. 2021

Dokl Biochem Biophys

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The effect of T-DNA insertion in the 3'-UTR region of Arabidopsis thaliana At3g58450 gene encoding the Germination-Related Universal Stress Protein (GRUSP) was studied. It was found that under a long-day condition this mutation delays transition to flowering of grusp-115 transgenic line that due to a reduced content of endogenous bioactive gibberellins GA1 and GA3 in comparison to the wild-type plants (Col-0). Exogenous GA accelerated flowering of both lines but did not change the time of difference in the onset of flowering between Col-0 and grusp-115. In addition to changes in GA metabolism, grusp-115 evidently has disturbances in realization of the signal that induces flowering. This is confirmed by the results of gene expression of the floral integrator FLOWERING LOCUS T (FT) and the floral repressor FLOWERING LOCUS C (FLC), which are key flowering regulators and acting opposite. We hypothesize that the formation of grusp-115 phenotype can also be affected by a low expression level of FT due to up-regulated FLC expression.

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The Gene Encoding the Universal Stress Protein AtUSP is Regulated by Phytohormones and Involved in Seed Germination of Arabidopsis thaliana

Abstract: For the first time, the organ-specific expression pattern of the AtUSP (At3g58450) gene, which also undergoes hormonal regulation, was shown. The USP protein encoded by this gene is involved in seed germination of Arabidopsis thaliana and, unlike abscisic acid, stimulates this process.

Cited by 16 publications

References 14 publications

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Universal Stress Protein regulates the circadian rhythm of central oscillator genes in Arabidopsis

GRUSP, an Universal Stress Protein, Is Involved in Gibberellin-dependent Induction of Flowering in Arabidopsis thaliana

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