The gelatin-based liquid marbles for cell cryopreservation

Liquid marbles: review of recent progress in physical properties, formation techniques, and lab-in-a-marble applications in microreactors and biosensors

“…145 (D) Cryopreservation of cells using gelatine-based LMs and its mechanism. 146 DryCell technology is based on cell-suspension microscale LMs for the analysis and manipulation of (E) single and (F) multiple heterogeneous cells. 13 (G) LM-based scaffold-free bioreactor for the 3D culture of in vitro derived human cardiomyocytes.…”

“…7D). 146 They fabricated LMs composed of gelatine solution and hydrophobic PTFE powder, where the PTFE particle layer not only acted as a physical barrier to protect cells from the external environment, but also influenced ice formation and growth during the freeze–thaw process of the solution. Furthermore, LMs containing gelatine in the liquid phase do not require additives, such as dimethyl sulfoxide (DMSO), which is cytotoxic, and foetal bovine serum (FBS), which may cause pathogenic contamination, like conventional cell cryopreservation; gentle shaking immediately destroys LMs and allows recovery of cells, thereby achieving high cell viability after cryopreservation.…”

“…(C) Isoelectric focusing (IEF)-based protein analysis using fluid channels composed of deformable LM, termed liquid plasticine 145. (D) Cryopreservation of cells using gelatine-based LMs and its mechanism 146. DryCell technology is based on cell-suspension microscale LMs for the analysis and manipulation of (E) single and (F) multiple heterogeneous cells 13.…”

Cell or cell derivative-laden hydrogels for myocardial infarction therapy: from the perspective of cell types

“…33,95 As reported, gelation and cryo-gelation hydrogels are less toxic to adipose-derived mesenchymal cells than conventional covalent cross-linked and photopolymerized hydrogels, indicating they are a more promising tool in vivo application. 96,97 On the other hand, the physical and mechanical properties of hydrogels are crucial for their application in stem cell tissue engineering. 98 Although the hydrogels have a stable three-dimensional porous structure for encapsulating cells or cell-derived agents, the biotherapeutic adding will inevitably affect the macroscopic properties of hydrogels.…”

Perturbation of Hydrogen Bonding Network Plays an Important Role in Determining Antifreeze Activity

“…In previous studies, we have identified some novel antifreeze agents and discussed corresponding mechanism. − Herein, we choose histidine and phenylalanine as two model molecules to investigate their IRI activity through experiment and molecular dynamics (MD) simulation. Both are α-amino acids containing an alanine moiety and one aromatic ring.…”