The GATA and SORLIP motifs in the 3-hydroxy-3-methylglutaryl-CoA reductase promoter of Picrorhiza kurrooa for the control of light-mediated expression

Abstract

Light upregulates the expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in Picrorhiza kurrooa, an endangered medicinal herb. Upstream sequences of HMGR of P. kurrooa (PropkHMGR) were analyzed in relation to its role in light-mediated regulation of gene expression. GATA motif in PropkHMGR exhibited stronger DNA-protein interaction with the nuclear extract of dark-exposed plants in contrast to SORLIP that exhibited stronger binding with the nuclear extract of light-exposed plants. Analysis of PropkHMGR (PropkHMGR-D1, -1,059/-1) and its deletion fragments PropkHMGR-D2 (-825/-1), PropkHMGR-D3 (-651/-1), PropkHMGR-D4 (-452/-1), and PropkHMGR-D5 (-101/-1) in Arabidopsis thaliana showed PropkHMGR to regulate gene expression [β-glucuronidase (GUS) was used as a reporter gene] at all the developmental stages but only in actively dividing tissues, excluding anthers. Whereas, PropkHMGR-D2 regulated GUS expression in relatively older seedlings but the expression was observed only in shoot apical meristem, root tips, and anthers. PropkHMGR-mediated gene expression was higher in dark as compared to that in the light in Arabidopsis across four temperatures studied. As opposed to the results in P. kurrooa, GATA motifs exhibited DNA-protein interaction with nuclear extract of light-exposed plants of Arabidopsis. SORLIP motifs in Arabidopsis also exhibited DNA-protein interaction with nuclear extract of light-exposed plants as in P. kurrooa. Data showed that (1) PropkHMGR regulated light-mediated gene expression and (2) GATA motif exhibited an inverse relationship between strength of DNA-protein interaction and the gene expression whereas the relationship was species specific for SORLIP.

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes

“…Light inhibits HMGR activity, while dark induces HMGR gene expression. The response of HMGR to light is low, and this process is mediated by light-responsive elements such as GATA and SORLIP motifs [52,64]. However, in this study, ultraviolet light up-regulated the expression of VvHMGR3, which means that light and ultraviolet light have different regulatory effects onHMGR activity.…”

Abscisic Acid Regulates the 3-Hydroxy-3-methylglutaryl CoA Reductase Gene Promoter and Ginsenoside Production in Panax quinquefolium Hairy Root Cultures

“…The presence of G-box and Box 4 elements in the PqHMGR promoter, possibly participating in the light response, has been confirmed. The light-responsive cis -elements have previously been found in promoters of HMGR genes from tomato, maidenhair tree ( Gingko biloba ), mulberry ( Morus alba ), kutki/kutaki ( Picrorhiza kurroa ), red sage ( Salvia miltiorrhiza ), and Andrographis paniculata [36,37,38,39,40,41]. The presence of a G-BOX element suggests that the isolated PqHMGR promoter may be homologous to PgHMGR1 .…”

Stress-Responsive cis-Regulatory Elements Underline Podophyllotoxin Biosynthesis and Better Performance of Sinopodophyllum hexandrum Under Water Deficit Conditions

“…The Genome Walking approach, thus, obtained a 1,034-bp 5 -upstream region of ShPLR (Figure 1 and Supplementary Figure 2), and a 785-bp region for ShSLD (Figure 2 and Supplementary Figure 2). Our previous experience in characterizing promoters of similar lengths in other plant species suggested that the present length of promoters was large enough to provide useful regulatory sequences controlling gene expression (Kawoosa et al, 2010(Kawoosa et al, , 2014Bhuria et al, 2016). The promoter sequences were submitted to NCBI with accession numbers such as OK574340 for ShPLR and OK574341 for ShSLD.…”