SUMMARYSerum gastrin was measured by radio-immunoassay in normal subjects and in patients with duodenal ulcer during insulin hypoglycaemia with and without concomitant aspiration of gastric acid. Insulin hypoglycaemia alone caused a rise in serum gastrin from 8.0 to 45.0 pg. per ml. in patients with duodenal ulcer, which was significantly greater than the rise from 13.0 to 29.0 pg. per ml. in normal subjects. During gastric aspiration serum-gastrin levels remained elevated for longer periods. The maximum increase of serum gastrin after insulin during gastric aspiration provided an almost complete discrimination between patients with duodenal ulcer and normal subjects. This discrimination between the groups was complete when serum gastrin was measured during insulin hypoglycaemia combined with intragastric neutralization of acid. The timing of the peak serum gastrin and the lowest blood-glucose coincided, but the peak acid output occurred 15-30 minutes later. Serum gastrin was inversely correlated with blood-glucose. Blood-glucose decreased to significantly lower levels in unaspirated than in aspirated subjects.These studies suggest that patients with duodenal ulcer produce more gastrin than normal subjects in response to insulin hypoglycaemia (and, by inference, to vagal stimulation). The results support the concept of an increased functional G-cell mass in duodenal ulcer patients, and indicate the physiological roles of vagally released gastrin and of the acid inhibitory mechanism. Discriminant functions observed in the study could form the basis of tests for the presence of the duodenal ulcer diathesis. GASTRIN release in response to both food stimulation and insulin hypoglycaemia has been extensively studied in normal subjects (Korman, Soveny, and Hansky, 1971a, b) and in patients with duodenal ulcer (Hansky, Korman, Cowley, and Baron, 1971; Korman, Soveny, and Hansky, 1971c). It has been shown that concomitant acid secretion modifies the gastrin response to insulin hypoglycaemia (Hansky and others, 1971). However, the relationship between the stimulatory effects on acid secretion of direct vagal impulses, on the one hand, and of gastrin, on the other, is unknown. In an attempt to resolve this question and to study further the effect of acid inhibition on gastrin release, the serum-gastrin response to insulin hypoglycaemia has been investigated, with and without simultaneous aspiration of gastric juice, both in normal subjects and in patients with duodenal ulcer.
MATERIALS AND METHODSSeventeen patients, comprising 15 men and 2 women of mean age 39 years, with radiologically proven duodenal ulcer were studied. These were compared with 11 normal subjects: 10 men and I woman of mean age 34 years. All the investigations were performed after an overnight fast. Venous blood was collected for gastrin and glucose estimation 30 minutes before and at 15-minute intervals for 2 hours after the rapid intravenous injection of soluble insulin (Insulin B.P., Boots), 0.2 U. per kg. body-weight.