The Galactose-Binding Lectin Isolated from Vatairea macrocarpa Seeds Enhances the Effect of Antibiotics Against Staphylococcus aureus–Resistant Strain

Santos, Valdenice F.; Costa, Maria do Socorro; Campina, Fábia F.; Rodrigues, Renato R.; Santos, Ana L.E.; Pereira, Felipe M.; Batista, Karla Lílian Rodrigues; Silva, Rafael C.; Pereira, Raquel Oliveira; Rocha, B.A.M.; Coutinho, Henrique Douglas Melo; Teixeira, Claudener S.

doi:10.1007/s12602-019-9526-z

Cited by 14 publications

(6 citation statements)

References 42 publications

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“…In the first study, the lectin extracted from the seeds of Dioclea violet (DVL) was evaluated, and although it did not present any clinical activity when evaluated alone (MIC ≥ 1024 μg/mL), DVL–gentamicin combination increased the antibiotic activity expressively, reducing the gentamicin MIC from 50.8 to 10.1 μg/mL against MDR S. aureus , and from 32 to 12.7 μg/mL against MDR E. coli [ 56 ]. In the second study, similar results were observed when Santos et al [ 57 ] evaluated the lectin extracted from Vatairea macrocarpa (VML) seeds (MIC ≥ 1024 μg/mL); furthermore, when combined with gentamicin, norfloxacin, and penicillin, an increase in antibiotic activity was also seen, with an MIC reduction of 512 to 128 μg/mL, 40.3 to 32 μg/mL, and 512 to 406.4 μg/mL, respectively, against MDR S. aureus [ 57 ].…”

Section: Biological Activities Of Lectinssupporting

confidence: 87%

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

2021

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Antibiotic monotherapy may become obsolete mainly due to the continuous emergence of resistance to available antimicrobials, which represents a major uncertainty to human health. Taking into account that natural products have been an inexhaustible source of new compounds with clinical application, lectins are certainly one of the most versatile groups of proteins used in biological processes, emerging as a promising alternative for therapy. The ability of lectins to recognize carbohydrates present on the cell surface allowed for the discovery of a wide range of activities. Currently the number of antimicrobials in research and development does not match the rate at which resistance mechanisms emerge to an effective antibiotic monotherapy. A promising therapeutic alternative is the combined therapy of antibiotics with lectins to enhance its spectrum of action, minimize adverse effects, and reduce resistance to treatments. Thus, this review provides an update on the experimental application of antibiotic therapies based on the synergic combination with lectins to treat infections specifically caused by multidrug-resistant and biofilm-producing Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. We also briefly discuss current strategies involving the modulation of the gut microbiota, its implications for antimicrobial resistance, and highlight the potential of lectins to modulate the host immune response against oxidative stress.

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Section: Biological Activities Of Lectinssupporting

confidence: 87%

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

2021

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“…SteLL at 16 µg/mL (8 × MIC) could also increase the bactericidal properties of ciprofloxacin, however, this action was only observed during the first hours. The synergistic interactions with antibiotics have been reported only for few plant lectins, including the lectins extracted from Alpinia purpurata (ApuL) 24 and Vatairea macrocarpa (VML) 48 .…”

Section: Discussionmentioning

confidence: 99%

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Lima

Zagmignan

Santos

et al. 2019

Sci Rep

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Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

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“…Apart from the bichromophoric nature of the two BODIPY derivatives, which is assessed thoroughly in this paper by through photophysical characterizations, the luminescent glycoconjugates GalTEBB-1 and GalTEBB-2 were conceived as derivatives targeting bacterial infections [15], with the luminescent portion constituted by the OPE and the two BODIPY residues, known in the literature as antibacterial materials [16], as well as the galactosyl moieties chosen for the capacity of several galactose-binding lectins [17,18] to modulate antibiotic activity against the Staphylococcus (S.) aureus and Escherichia coli bacterial strains [15]. New drugs and efficient strategies are required to control bacteriaassociated infections due to the emergence and rapid dissemination of antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Bodipy-Tagged Galactoconjugates and Evaluation of Their Antibacterial Properties

Gangemi,

Monforte,

Arrigo

et al. 2024

Molecules

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As a development of our research on biocompatible glycoconjugate probes and specifically multi-chromophoric systems, herein, we report the synthesis and early bactericidal tests of two luminescent glycoconjugates whose basic structure is characterized by two boron dipyrromethene difluoride (BODIPY) moieties and three galactoside rings mounted on an oligophenylene ethynylene (OPE) skeleton. BODIPY fluorophores have found widespread application in many branches of biology in the last few decades. In particular, molecular platforms showing two different BODIPY groups have unique photophysical behavior useful in fluorescence imaging. Construction of the complex architecture of the new probes is accomplished through a convergent route that exploits a series of copper-free Heck–Cassar–Sonogashira cross-couplings. The great emergency due to the proliferation of bacterial infections, in conjunction with growing antibiotic resistance, requires the production of new multifunctional drugs and efficient methods for their targeted delivery to control bacteria-associated diseases. Preliminary studies of the glycoconjugate properties as antibacterial agents against representatives of Gram-negative (P. aeruginosa) and Gram-positive (S. aureus) pathogens, which are associated with chronic infections, indicated significant bactericidal activity ascribable to their structural features.

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The Galactose-Binding Lectin Isolated from Vatairea macrocarpa Seeds Enhances the Effect of Antibiotics Against Staphylococcus aureus–Resistant Strain

Cited by 14 publications

References 42 publications

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

Potential Application of Combined Therapy with Lectins as a Therapeutic Strategy for the Treatment of Bacterial Infections

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Synthesis of Bodipy-Tagged Galactoconjugates and Evaluation of Their Antibacterial Properties

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