The G protein β subunit FGB1 regulates development and pathogenicity in Fusarium oxysporum

Jain, Sona; Akiyama, Kouichi; Kan, Takuya; Ohguchi, Tomizo; Takata, Renkichi

doi:10.1007/s00294-003-0372-9

Cited by 72 publications

(52 citation statements)

References 39 publications

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“…The number of VAGs in Foc1 and Foc4 is comparable to that in the Foc race 4 strain II5 (357) and other sequenced fungi (Table S4 & Table S5 in File S1). The search of the PHI database yielded 16 already characterized VAGs from Fo, including ARG1 [8], CHS2 [9], CHSV [10], CHS7 [9], FMK1 [11], FGA1 [12], FGA2 [13], FGB1 [14], FOW1 [15], FOW2 [16], foSNF1 [17], FRP1 [18], GAS1 [19], PacC [20] and SIX1 [21], indicating conservation in VAGs and pathogenesis among the different formae speciales of Fo. A number of Foc orthologs were found in the cereal pathogenic fungi F. graminearum (13 genes), Magnaporthe oryzae (88) and Ustilago maydis (31).…”

Section: Resultsmentioning

confidence: 99%

“…Also, GprD in Aspergillus fumigatus was suggested as an essential regulator of colony growth, hyphal morphogenesis, and virulence [95]. In Fo, the roles of GPCRs in G protein signaling pathway were not characterized, but G protein alpha and beta subunit were elucidated to be involved in growth, development and pathogenicity (FGA1 [12], FGA2 [13], FGB1 [14]). We revealed ∼115 genes encoding the putative GPCRs (including Pth11 like) and G proteins in Foc (Table S9 in File S1).…”

Section: Resultsmentioning

confidence: 99%

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Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

et al. 2014

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BackgroundThe asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced.Methodology/Principal FindingsGenome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana ‘Brazil’ in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety ‘Brazil’.Conclusions/SignificanceFoc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

et al. 2014

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“…Two G α subunits from F. oxysporum , FGA1 and FGA2 , and the G β subunit from F. oxysporum , FGB1 , and G. moniliformis , GBB1 , have been functionally characterized (Delgado-Jarana et al , 2005; Jain et al , 2002, 2003, 2005; Sagaram & Shim, 2007). However, they do not have direct parallels in Gibberella ( Fusarium ) species.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, G β subunits in Fusarium oxysporum and Gibberella moniliformis differ only in one amino acid, even though their functional roles in plant pathogenesis are quite different. Jain et al (2003) showed that F. oxysporum FGB1 , a gene encoding a G β subunit, is involved in virulence, while G. moniliformis GBB1 has no impact on maize stalk rot pathogenesis (Sagaram & Shim, 2007). The availability of three Fusarium genomes enables comparative functional analysis of heterotrimeric G protein subunits in G .…”

Section: Introductionmentioning

confidence: 99%

Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae

et al. 2008

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The homothallic ascomycete fungus Gibberella zeae (anamorph: Fusarium graminearum) is a major toxigenic plant pathogen that causes head blight disease on small-grain cereals. The fungus produces the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in infected hosts, posing a threat to human and animal health. Despite its agricultural and toxicological importance, the molecular mechanisms underlying its growth, development and virulence remain largely unknown. To better understand such mechanisms, we studied the heterotrimeric G proteins of G. zeae, which are known to control crucial signalling pathways that regulate various cellular and developmental responses in fungi. Three putative Gα subunits, GzGPA1, GzGPA2 and GzGPA3, and one Gβ subunit, GzGPB1, were identified in the F. graminearum genome. Deletion of GzGPA1, a homologue of the Aspergillus nidulans Gα gene fadA, resulted in female sterility and enhanced DON and ZEA production, suggesting that GzGPA1 is required for normal sexual reproduction and repression of toxin biosynthesis. The production of DON and ZEA was also enhanced in the GzGPB1 mutant, suggesting that both Gα GzGPA1 and Gβ GzGPB1 negatively control mycotoxin production. Deletion of GzGPA2, which encodes a Gα protein similar to A. nidulans GanB, caused reduced pathogenicity and increased chitin accumulation in the cell wall, implying that GzGPA2 has multiple functions. Our study shows that G. zeae heterotrimeric G protein subunits can regulate vegetative growth, sexual development, toxin production and pathogenicity.

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“…It is interesting to note that the requirement of the Gβ and Gγ subunits for infection in different fungal plant pathogens varies. For example, it has been previously demonstrated that GBB1 in Gibberella monoliformis is not required for pathogenicity whist the orthologous protein in the related Fusarium oxysporum is [19,20]. Our data clearly show that gene encoding for the Gβ subunit, Gba1 , is required for S. nodorum to cause disease on wheat.…”

Section: Discussionmentioning

confidence: 52%

A comparative analysis of the heterotrimeric G-protein Gα, Gβ and Gγ subunits in the wheat pathogen Stagonospora nodorum

et al. 2012

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BackgroundIt has been well established that the Gα subunit of the heterotrimeric G-protein in the wheat pathogen Stagonospora nodorum is required for a variety of phenotypes including pathogenicity, melanisation and asexual differentiation. The roles though of the Gγ and Gβ subunits though were unclear. The objective of this study was to identify and understand the role of these subunits and assess their requirement for pathogenicity and development.ResultsG-protein Gγ and Gβ subunits, named Gga1 and Gba1 respectively, were identified in the Stagonospora nodorum genome by comparative analysis with known fungal orthologues. A reverse genetics technique was used to study the role of these and revealed that the mutant strains displayed altered in vitro growth including a differential response to a variety of exogenous carbon sources. Pathogenicity assays showed that Stagonospora nodorum strains lacking Gba1 were essentially non-pathogenic whilst Gga1-impaired strains displayed significantly slower growth in planta. Subsequent sporulation assays showed that like the previously described Gα subunit mutants, both Gba1 and Gga1 were required for asexual sporulation with neither mutant strain being able to differentiate either pycnidia nor pycnidiospores under normal growth conditions. Continued incubation at 4°C was found to complement the mutation in each of the G-protein subunits with nearly wild-type levels of pycnidia recovered.ConclusionThis study provides further evidence on the significance of cAMP-dependent signal transduction for many aspects of fungal development and pathogenicity. The observation that cold temperatures can complement the G-protein sporulation defect now provides an ideal tool by which asexual differentiation can now be dissected.

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The G protein β subunit FGB1 regulates development and pathogenicity in Fusarium oxysporum

Cited by 72 publications

References 39 publications

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

Functional analyses of heterotrimeric G protein Gα and Gβ subunits in Gibberella zeae

A comparative analysis of the heterotrimeric G-protein Gα, Gβ and Gγ subunits in the wheat pathogen Stagonospora nodorum

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