The G protein-coupled receptors in the silkworm, Bombyx mori

Fan, Yi; Sun, Peng; Wang, Yu; He, Xiaobai; Deng, Xiaoyan; Chen, Xiaopan; Zhang, Guozheng; Chen, Xin; Zhou, Naiming

doi:10.1016/j.ibmb.2010.05.005

Cited by 76 publications

(95 citation statements)

References 73 publications

(88 reference statements)

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“…Similar efforts to systematically annotate GPCRs and/or their neuropeptide ligands in nonvertebrate animals and relate them to known vertebrate or arthropod-type PS receptors have been confined to chordates (38,39) or insects (9,40,41). In one important study (42), researchers used HMMs to annotate eukaryotic GPCRs and place them into one of the GRAFS (Glutamate, Rhodopsin, Adhesion, Frizzled, Secretin) classes.…”

Section: Discussionmentioning

confidence: 99%

Molecular evolution of peptidergic signaling systems in bilaterians

Mirabeau

Joly

2013

Proc. Natl. Acad. Sci. U.S.A.

369

707

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Peptide hormones and their receptors are widespread in metazoans, but the knowledge we have of their evolutionary relationships remains unclear. Recently, accumulating genome sequences from many different species have offered the opportunity to reassess the relationships between protostomian and deuterostomian peptidergic systems (PSs). Here we used sequences of all human rhodopsin and secretin-type G protein-coupled receptors as bait to retrieve potential homologs in the genomes of 15 bilaterian species, including nonchordate deuterostomian and lophotrochozoan species. Our phylogenetic analysis of these receptors revealed 29 wellsupported subtrees containing mixed sets of protostomian and deuterostomian sequences. This indicated that many vertebrate and arthropod PSs that were previously thought to be phyla specific are in fact of bilaterian origin. By screening sequence databases for potential peptides, we then reconstructed entire bilaterian peptide families and showed that protostomian and deuterostomian peptides that are ligands of orthologous receptors displayed some similarity at the level of their primary sequence, suggesting an ancient coevolution between peptide and receptor genes. In addition to shedding light on the function of human G protein-coupled receptor PSs, this work presents orthology markers to study ancestral neuron types that were probably present in the last common bilaterian ancestor. neuropeptide evolution | GPCR evolution | bilaterian CNS cell types | bilaterian brain evolution

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Section: Discussionmentioning

confidence: 99%

Molecular evolution of peptidergic signaling systems in bilaterians

Mirabeau

Joly

2013

Proc. Natl. Acad. Sci. U.S.A.

369

707

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“…Recently, two putative AKH receptors, BNGR-A28 and -A29, have been identified by using the previously identified Drosophila and Bombyx AKH receptors (13) for BLAST searches of the genomic databases from Bombyx (20,21). Phylogenetic analysis has shown that the Bombyx AKHR is grouped into "Arthropoda-AKHR cluster" together with recently identified AKHRs of Drosophila, cockroach and mosquito, whereas BNGR-A28 and -A29 have been grouped into "Arthropoda-AKHR-like cluster" containing putative receptors of all three mosquito species, T. castaneum, and the parasitoid wasp Nasonia vitripennis (32).…”

Section: Discussionmentioning

confidence: 99%

“…Bombyx neuropeptide GPCR A28 and A29 (BNGR-A28 and A29) have been identified as adipokinetic hormone-like receptors in Bombyx by genomic data mining and phylogenetic analysis (20,21). The predicted genes of BNGR-A28 (GenBank TM accession number AB330449) and -A29 (GenBank TM accession number AB330450) were cloned by RT-PCR from brain and testis of silkworm larva, respectively.…”

Section: Cloning and Expression Of Bngr-a28 And Bngr-a29 In Hek293mentioning

confidence: 99%

“…By genomic data mining and phylogenetic analysis, Bombyx neuropeptide GPCR A28 and A29 (BNGR-A28 and -A29) have been identified as adipokinetic hormone-like receptors in Bombyx (20,21). Therefore, in this study, we report on the cloning of cDNAs encoding the putative adipokinetic hormone-like receptors BNGR-A28 and BNGR-A29 from the silkworm B. mori and functional expression of both receptors in HEK293 cells.…”

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confidence: 99%

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Identification and Functional Characterization of Two Orphan G-protein-coupled Receptors for Adipokinetic Hormones from Silkworm Bombyx mori

Shi

Huang

Deng

et al. 2011

Journal of Biological Chemistry

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Background: AKH/AKHR system is of importance for insects to control energy homeostasis. Results: Bombyx BNGR-A28 and BNGR-A29 were activated in signaling and internalization by AKH3 at high affinity but not by corazonin. Conclusion: AKH3 was a specific ligand for two orphan GPCRs, AKHR2a and AKHR2b. Significance: Our findings will lead to a better understanding of the AKH/AKHR system in regulation of fundamental physiological processes.

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“…GPCRs that have been deorphanized are followed by their ligand in the figure. A few other GPRs are indicated by the genus name of the species and the their gen bank accession number; in the latter cases sequences were sometimes corrected. The Bombyx LRG1 (NP_001037033) and LRG2 sequence were used previously (Fan et al, 2010). Nodes with an approximate maximum-likelihood estimate (Price et al, 2010) of between 0.96 and 1.00 are indicated with a solid black circle, those between 0.90 and 0.95 with an open circle.…”

Section: Eflamidementioning

confidence: 99%