The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle

Abstract

The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.

Genome‐wide association study for carcass traits, fatty acid composition, chemical composition, sugar, and the effects of related candidate genes in Japanese Black cattle

“…(), or association test in many populations with different genetic background such as in Hayakawa et al . (). However, our study performed GWAS with many traits that were difficult to measure, and both methods could not be applicable.…”

“…Recently, the g.841G > C mutation in FASN showed stronger association with fatty acid composition than those of g.16024A > G (Hayakawa et al . ). The g.16024A > G mutation was not related to carcass traits (Matsuhashi et al .…”

Genome wide association study and genomic prediction for fatty acid composition in Chinese Simmental beef cattle using high density SNP array

“…Previous studies based on candidate gene approaches had revealed polymorphisms within FASN that were related to fatty acid composition in multiple beef cattle populations [45–49] and milk fat content in dairy cattle [50, 51]. For instance, several studies were conducted to explore and evaluate the association between fatty acid composition and candidate SNPs using GWAS in Japanese beef cattle [8, 30, 52]. These studies provided multiple evidences that several SNPs near or within FASN may be regarded as responsible mutations for fatty acid composition and contribute largely to meat quality in the Japanese Black cattle population.…”

Epistatic effects between pairs of the growth hormone secretagogue receptor 1a, growth hormone, growth hormone receptor, non‐SMC condensin I complex, subunit G and stearoyl‐CoA desaturase genes on carcass, price‐related and fatty acid composition traits in Japanese Black cattle

“…Further, Hayakawa et al . () revealed that the g.841G>C SNP of FASN gene had significant associations with FA composition traits (C14:0, C14:1, C16:1, C18:1). Therefore, the A haplotype of GH locus would be responsible for the presence of C14:0 as well as the g.841G>C SNP of FASN gene in JBK, at least in S.…”