The fungus Pestalotiopsis sp., isolated from Perna perna (Bivalvia:Mytilidae) cultured on marine farms in Southeastern Brazil and destined for human consumption

Santos, A. L.; Medeiros, João Victor Ferreira de; Grault, Carlos Eduardo; Santos, Manoel J.S.; Souza, André Luiz Almeida; Carvalho, Raimundo Wilson de

doi:10.1016/j.marpolbul.2020.110976

Cited by 4 publications

(1 citation statement)

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“…In Brazil, cultured brown mussels Perna perna were found contaminated with Pestalotiopsi sp. [ 16 ], as well as farmed and wild mussels Mytulis galloprovincialis in the Adriatic Sea [ 17 ]. In the Sea of Japan (Russia), the contamination of yesso scallop Mizuhopecten yessoensis , Pacific oyster Crassostrea gigas , and bay mussel M .…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Genotoxic Potential of the Marine Toxin C17-SAMT Using the In Vivo Comet and Micronucleus Assays

et al. 2022

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The contaminant responsible for the atypical toxicity reported in mussels from Bizerte Lagoon (Northern Tunisia) during the last decade has been characterized as C17-sphinganine analog mycotoxin (C17-SAMT). This neurotoxin showed common mouse toxic symptoms, including flaccid paralysis and severe dyspnea, followed by rapid death. For hazard assessment on human health, in this work we aimed to evaluate the in vivo genotoxic effects of this marine biotoxin using the classical alkaline and modified Fpg comet assays performed to detect DNA breaks and alkali-labile sites as well as oxidized bases. The micronucleus assay was used on bone marrow to detect chromosome and genome damage. C17-SAMT induces a statistically insignificant increase in DNA tail intensity at all doses in the duodenum, and in the spleen contrary to the liver, the percentage of tail DNA increased significantly at the mid dose of 300 µg/kg b.w/d. C17-SAMT did not affect the number of micronuclei in the bone marrow. Microscopic observations of the liver showed an increase in the number of mitosis and hepatocytes’ cytoplasm clarification. At this level of study, we confirm that C17-SAMT induced DNA damage in the liver but there was no evidence of effects causing DNA oxidation or chromosome and genome damage.

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Section: Introductionmentioning

confidence: 99%