The Fungicidal Terpenoids and Essential Oil from Litsea cubeba in Tibet

Abstract

A new C9 monoterpenoid acid (litseacubebic acid, 1) and a known monoterpene lactone (6R)-3,7-dimethyl-7-hydroxy-2-octen-6-olide (2), along with three known compounds – vanillic acid (3), trans-3,4,5-trimethoxylcinnamyl alcohol (4), and oxonantenine (5) – were isolated with bioassay-guided purification from the fruit extract of Litsea cubeba collected in Tibet. The structure of 1 was elucidated by MS, 1H-NMR, 13C-NMR, COSY, HSQC, HMBC, NOE spectral data as 2,6-dimethyl-6-hydroxy-2E,4E-hepta-2,4-diene acid. Additionally 33 compounds were identified from the essential oil of L. cubeba. The preliminary bioassay results showed that 1 and 2 have good fungicidal activities against Sclerotinia sclerotiorum, Thanatephorus cucumeris, Pseudocer-cospora musae and Colletotrichum gloeosporioides at the concentration of 588 and 272 μM, and the essential oil has good fungicidal activities against T. cucumeris and S. sclerotiorum, with IC50 values of 115.58 and 151.25 μg/mL, repectively.

Inhibitory Effect of Herbal Remedy PERVIVO and Anti-Inflammatory Drug Sulindac on L-1 Sarcoma Tumor Growth and Tumor Angiogenesis in Balb/c Mice

“…Phenylpropanoids of Alpinia galanga was efflux pump inhibitors in Mycobacterium smegmatis mc 2 155 cells [41]. Essential oils from Litsea cubeba contain fungicidal and antibacterial terpenoids [42, 43]. Sesquiterpene lactones from Inula helenium root essential oil exhibited antistaphylococcal activity [44].…”

Antibacterial activity ofLitsea cubeba(Lauraceae, May Chang) and its effects on the biological response of common carpCyprinus carpiochallenged withAeromonas hydrophila

“…A large variety of chemotypes of L. cubeba essential oils (EOs) has been described for different parts of the plant (root, stem, leaves, fruit, flowers) in different countries such as China, Taiwan, Vietnam, Tibet (Yang et al . ; Si et al . ; Li et al .…”

“…It has been used in TCM for headache, fatigue, muscle pain and depression; and fresh leaves were mashed and used for skin problems, such as sore and furuncles (Bhuinya et al 2010;Chen et al 2013). A large variety of chemotypes of L. cubeba essential oils (EOs) has been described for different parts of the plant (root, stem, leaves, fruit, flowers) in different countries such as China, Taiwan, Vietnam, Tibet (Yang et al 2010;Si et al 2012;Li et al 2014). The major compound found in leaf essential oils was either 1,8-cineole, sabinene, linalool or neral with a broad chemical variability among sampled regions (Bighelli et al 2005;Wang and Liu 2010;Son et al 2014).…”

Antibacterial, Antifungal and Cytotoxic Isoquinoline Alkaloids from Litsea cubeba

“…The bark of L. cubeba has been used in oriental traditional medicine for the treatment of atopic eczema and coronary heart disease, and its antioxidant activities and antimicrobial activities against Staphylococcus aureus , Salmonella typhi , and Pseudomonas aeruginosa have been reported [5,6,7]. The essential oil from L. cubeba has good fungicidal activities against Sclerotinia sclerotiorum , Thanatephorus cucumeris , Pseudocercospora musae and Colletotrichum gloeosporioides [8,9]. Various types of alkaloids have been isolated from this plant [10,11,12].…”