The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Liu, Gongyan; Li, Shu; Liu, Hongli; Zhu, Yanli; Bai, Liya; Sun, Haitao; Gao, Shuxia; Jiang, Wenxue; Li, Fuchang

doi:10.21203/rs.2.13789/v6

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…These lncRNAs and genes might play crucial roles in regulating hair follicle density, and their differential expression might be the reason for differences in hair follicle density and wool production between HWP and LWP rabbits. Liu et al (2020) analysed the miRNA effect on hair follicle density in the Rex rabbit [37], but lncRNA related to hair density in rabbits has only been done in the present study.…”

Section: Discussionmentioning

confidence: 99%

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

Ding

Zhao

et al. 2021

Preprint

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Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive. Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR). Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.

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Section: Discussionmentioning

confidence: 99%

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

Ding

Zhao

et al. 2021

Preprint

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“…Previous studies have shown that there is a direct relationship between the Notch signal pathway and hair follicle morphogenesis [47], high expression of Notch1 and Notch2 can accelerate the formation of mouse hair substrate and, at the same time, it can inhibit the cells around hair substrate to form substrate [48]. Ocu-miR-205 can promote the transition of Rex rabbit hair follicles from the growing stage to a degenerative quiescent stage by regulating the expression of related genes and proteins in Notch, BMP and other signal pathways,changing the hair density [49]. Krieger et alused mice model to study the regulation of the NF-kb signal pathway in the hair follicle cycle, and found that the NF-kb signal pathway is essential for the growth and activation of hair follicle stem cells [50].…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goats Hair Follicles

Shang

Wang

et al. 2020

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Background:Inner Mongolian cashmere goats have hair of excellent quality and high economic value, and the skin hair follicle traits of cashmere goats have a direct and important effect on cashmere yield and quality.Circular RNA has been studied in a variety of tissues and cells. Result:In this study, high-throughput sequencing was used to obtain the expression profiles of circRNA in the hair follicles of Inner Mongolia cashmere goats at different embryonic stages (45, 55 , 65 and 75 days). A total of 21784 circRNAs were identified. At the same time, the differentially expression circRNA in the six control groups formed in the four stages were: d75vsd45, circRNA up-regulated by 59 and down-regulated by 33; d75vsd55, circRNA up-regulated by 61 and down-regulated by 102; d75vsd65, circRNA up-regulated by 32 and down-regulated by 33; d65vsd55, circRNA up-regulated by 67 and down-regulated by 169; d65vsd45, circRNA up-regulated by 96 and down-regulated by 63; and d55vsd45, circRNA up-regulated by 76 and down-regulated by 42. Six DE circRNA were randomly selected to verify the reliability of the sequencing results by qRT-PCR. Subsequently,the circRNA corresponding host genes were analyzed by GO and the KEGG pathaway. The results showed that the biological processes related to hair follicle growth and development enriched by GO mainly included hair follicle morphogenesis, hair follicle maturation and cell development, and the signal pathways related to hair follicle development included the Notch signaling pathway and NF-kappa B signaling pathway.We combined the DE circRNA of d75vsd45 with miRNA and mRNA databases(unpublished) to construct the co-expression network of circRNA-miRNA-mRNA, and formed a total of 102 pairs of circRNA-miRNA and 126 pairs of miRNA-mRNA interactions. The binding relationship of circRNA3236-chi-miR-27b-3p and circRNA3236-chi-miR-16b-3p was further verified by dual-luciferase reporter assays,and the results showed that circRNA3236 and chi-miR-27b-3p,circRNA3236 and chi-miR-16b-3p have a targeted binding relationship.Conclusion:To summarize, we established the expression profile of circRNA in the fetal skin hair follicles of cashmere goats, and found that the host gene of circRNA may be involved in the development of hair follicles of cashmere goats. The co-expression network of circRNA-miRNA-mRNA that DE circRNA was constructed and preliminary verification was carried out.

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“…The fur quality index in different ages of Rex rabbit detection method as described previously [13]. The adhesive tissue and fat was removed from fur, and weighed, then cut in the middle of the abdomen, and the length and width of the skin were measured using a soft ruler.…”

Section: Fur Qualitymentioning

confidence: 99%

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

Liu,

Zhang,

Bai

et al. 2024

Preprint

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In the present study, we aimed to observed hair follicle development and describe Wnt10b/β-catenin signaling pathways related to hair follicle development in different ages of Rex rabbits. Six Rex rabbits (3 male and 3 female) of 15, 30, 60, 90, 120 and 150 days of age were selected and skin samples were collected for hair follicle development and Wnt/β-catenin signaling pathway genes expresion detection. The results showed that the the values for body weight, coat length, skin thickness, skin area, and skin weight of Rex rabbits were increased significantly with age (p &lt; 0.05). The total hair follicle density, secondary hair follicle density and the ratio of the secondary hair follicle density to the primary hair follicle density in 120 days of Rex rabbits was higher than that in the other ages (p &lt; 0.05), and the major hair follicles were in the growing period at 15 days and 30 days of age, some of them had already entered the degeneration period at 60 days of age, and most of them entered the degeneration period at 90 days of age, the presence of quiescent hair follicles and the enlargement of primary hair follicles were also observed. At 120 and 150 days of age, the back hair follicles entered the growing period, and the secondary hair follicles had obvious differentiation. Furthermore, the expression of the Wnt10b, CTNNB1, adenomatosis polyposis protein (APC), Disheveled 2 (DVL2), glycogen synthase kinase 3β（GSK-3β）, Lymphoid enhancer-binding factor 1 (LEF1), dickkopf-1 (DKK1) and transforming growth factor beta 1 (TGFβ-1) genes in the skin tissue of Rex rabbits at different age was significantly differences (p &lt; 0.05), and the expression of the Wnt10b, CTNNB1, DVL2, GSK-3β, and TGFβ-1 genes at 90 days was higher than that in the other days, the expression of the LEF1 and DKK1 genes at 60 days was higher than that in the other days. As western blotting indicated, the β-catenin protein phosphorylation (P-CTNNB1) levels in the skin tissue of Rex rabbits shows the trend of first increasing, then decreasing and then increasing, 60 days, 120 days and 150 days significantly higher than 15 days, 30 days or 90 days (p &lt; 0.05). With the increasing of ages, the GSK-3β protein phosphorylation (P-GSK-3β) levels in the skin tissue of Rex rabbits were increasing, and maximum at 120 days of age (p &lt; 0.05). These results show that ages could influence on the fur quality and hair follicle traits of Rex rabbits, and Wnt10b/β-catenin signaling pathways is involved in the biological process of periodic hair follicle development in Rex rabbits. Considering the rule of fur growth, the slaughter age of Rex rabbits should not be earlier than 120 days.

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The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Cited by 4 publications

References 17 publications

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goats Hair Follicles

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

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