The Functional Role of PPN1 and PPX1 Polyphosphatases under Stresses Action and for Adaptive Response Development

Voychuk, S.I.; Gromozova, E. N.

doi:10.15407/microbiolj82.01.003

Cited by 2 publications

(4 citation statements)

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“…The action of acetic acid had the opposite effect on this sugar. The changes that were marked in the cell walls of The hydrogen peroxide was shown to be able to decrease the viability of the PPN1 deficient cells whereas the PPX1-deletion increased cell resistance to all stresses that we studied [8]. The RF-EMF (40.68 MHz) exposure was able to increase cell resistance to all stresses that happened, presumably, because of the induction of the adaptive response processes in the yeast cells.…”

Section: Change Of Sugar Content In the Cell Walls And Extracellular Matrix Of The Ppn1 And Ppx1 Deficient Cells Under The Action Of Strementioning

confidence: 77%

“…Saccharomyces cerevisiae yeast deficient in ppn1 and ppx1 (a wild type (Wt-cells), two single mutants (∆ppn1-cells and ∆ppx1-cells) and a double mutant (∆ppn1::∆ppx1-cells)) were used in the study [8]. Yeasts were grown on YEPD at 28° C for 16 ± 1 h. Cells were washed from nutrient residues with PBS (pH 7.2) by centrifugation at ×300g for 5 min, three times, and resuspended in sterile distilled water to a concentration of 2×10 8 cells/ml according to optical density.…”

Section: Yeast Strains and Cultivationmentioning

confidence: 99%

“…The treatment of cells with the radiofrequency electromagnetic field (RF-EMF) was used to initialize the adaptive response processes within the cells [8]. Yeast suspensions in the polypropylene 1.5 ml tubes were placed into the solenoid connected to the generator of EMF (40.68 MHz, 27.5 V/m, 22 A/m, the capacity of radiation 15 W, polarized in a horizontal plane).…”

Section: Rf-emf Treatmentmentioning

confidence: 99%

“…However, such assumptions are usually made under optimal conditions while many structural elements of cell (including its genetic and biochemical properties) serve to ensure cell functionality in the environment that is far from optimal. The biological functions that perform poly(P)ases PPN1 and PPX1 are probably even more important for cell resistance to the action of the stress factors [8].…”

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The Role of PPN1 and PPX1 Polyphosphatases in the Stress-Induced Changes of the Polysaccharide Composition of Cell Wall and Extracellular Matrix of Saccharomyces cerevisiae Cells

Voychuk¹,

Gromozova²

2020

Mikrobiol. Z.

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Polysaccharides (PS) are important structural elements of all living organisms. They perform many important functions and protect cells from the action of various stresses. The in vivo synthesis of PS is an energy-consuming process that requires phosphates, as well as the structure of PS requires the phosphates and polyphosphates (poly(P)) as binding elements. However, the role of enzymes that metabolize poly(P) (polyphosphatases, poly(P)ases) in the processes of cell wall components and extracellular matrix synthesis is poorly understood. Aim. The aim was to study the role of PPN1 and PPX1 poly(P)ases in the processes of cell wall and extracellular matrix formation. Methods. Saccharomyces cerevisiae yeast cells with deletions of PPN1 (Δppn1) and PPX1 (Δppx1) were used in the study. Cells were exposed to hydrogen peroxide (25-100 mM), acetic acid (25-100 mM) and sorbitol (0.25-1.0 M) to induce stress reactions. 15W power, 30 min) was applied separately and 30 min before treatment with other stress factors to induce an adaptive response. The influence of stress factors was evaluated by changes in the content of sugars. The sugars and sugar residues in the cell walls and extracellular matrix of the yeast cells were detected using GC/MS and lectin-gold binding test. The content of D-mannose/Dglucose (Man/Glu), D-galactose (Gal), N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc) and N-acetylneuraminic acid (NANA) was assessed. Results. The deficiency of PPN1 and PPX1 affect the content of sugars in the cell walls and extracellular matrix. The amounts of glucosamine (according to GC/MS analysis) decreased in 3-5 times in the cell walls of PPN1 and PPX1 deficient cells. The lectingold test showed that the Man/Glu content was the most stable (27-38%) among all the yeast cells, while the amounts of other sugars varied significantly. The deletion of poly(P)ases had different effects on the content of sugars in the cell walls and extracellular matrix: the extracellular matrix showed a significant decrease in GlcNAc, GalNAc and an increase in NANA, while the content of GalNAc in the cell walls remained almost constant, and the content of NANA decreased in case of PPN1 deletion and increased in case of PPX1 deletion. Correlation analysis showed a potentially high (up to 97%) correlation between Man/Glu, GlcNAc, and GalNAc amount in cell walls, and cell viability (stress-resistance). However, only one of these sugars, GlcNAc, showed correlation with deficiency of PPN1 and PPX1. The differences between the effects observed in cells with single and double deletions of poly(P)ases indicate that both enzymes positively regulate GlcNAc biosynthesis of cell walls and extracellular matrix and the biosynthesis of extracellular GalNAc. Conclusions. Both poly(P)ases (PPN1 and PPX1) are involved in the assembly of the cell wall and extracellular matrix and influence mainly the content of their minor constituents: Gal, GalNAc, GlcNAc, and NANA. The change in stresses resistance of PPN1 and PPX1 deficient cells correlate with...

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Section: Change Of Sugar Content In the Cell Walls And Extracellular Matrix Of The Ppn1 And Ppx1 Deficient Cells Under The Action Of Strementioning

confidence: 77%

Section: Yeast Strains and Cultivationmentioning

confidence: 99%

Section: Rf-emf Treatmentmentioning

confidence: 99%

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confidence: 99%

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