The Functional Role of Long Non-Coding RNA in Myogenesis and Skeletal Muscle Atrophy

Hitachi, Keisuke; Honda, Masahiko; Tsuchida, Kunihiro

doi:10.3390/cells11152291

Cited by 12 publications

(12 citation statements)

References 154 publications

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“…Currently, most studies on muscle atrophy have used aged mouse models or cells to date, but whether human counterparts have similar molecular functions in muscle mass remains unclear [72]. Hence, the potential applicability of these investigated factors for the treatment of muscle atrophy in humans should be carefully evaluated.…”

Section: Discussionmentioning

confidence: 99%

lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p

Yu,

He,

Liu

et al. 2023

Aging

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Sarcopenia induced by muscle aging is associated with negative outcomes in a variety of diseases. Long noncoding RNAs are a class of RNAs longer than 200 nucleotides with lower protein coding potential. An increasing number of studies have shown that lncRNAs play a vital role in skeletal muscle development. According to our previous research, lncRNA GPRC5D-AS1 is selected in the present study as the target gene to further study its effect on skeletal muscle aging in a dexamethasone-induced human muscle atrophy cell model. As a result, GPRC5D-AS1 functions as a ceRNA of miR-520d-5p to repress cell apoptosis and regulate the expression of muscle regulatory factors, including MyoD, MyoG, Mef2c and Myf5, thus accelerating myoblast proliferation and differentiation, facilitating development of skeletal muscle. In conclusion, lncRNA GPRC5D-AS1 could be a novel therapeutic target for treating sarcopenia.

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Section: Discussionmentioning

confidence: 99%

lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p

Yu,

He,

Liu

et al. 2023

Aging

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“…This pathological process causes abnormal ossification of ligaments in accordance with the forementioned mechanotransduction-related processes [ 34 ]. Noncoding ribonucleic acids (RNA), which do not translate into protein, have been increasingly recognized for their involvement in various biological processes, including bone metabolism and muscle atrophy [ 35 - 38 ]. For instance, long noncoding RNA H19, closely associate with BMP9-induced osteogenic differentiation in mesenchymal stem cells in OPLL, is also related to muscle atrophy process [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation Between the Severity of Multifidus Fatty Degeneration and the Size of Ossification of Posterior Longitudinal Ligament at Each Spinal Level

Park,

Cho,

Kim

et al. 2023

Neurospine

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Objective: This study aimed to investigate the correlation between ossification of the posterior longitudinal ligament (OPLL) size and multifidus fatty degeneration (MFD), hypothesizing that larger OPLL sizes are associated with worse MFD.Methods: One hundred four patients with cervical OPLL who underwent surgery were screened. OPLL occupying diameter and area ratios, the severity of MFD using the Goutallier classification, and range of motion (ROM) of cervical flexion-extension (ΔCobb) were measured. Correlation analyses between OPLL size, MFD severity, and ΔCobb were conducted. MFD severity was compared for each OPLL type using one-way analysis of variance.Results: The final clinical data from 100 patients were analyzed. The average Goutallier grade of C2–7 significantly correlated with the average OPLL diameter and area occupying ratios, and OPLL involved vertebral level (r = 0.58, p < 0.01; r = 0.40, p < 0.01; r = 0.47, p < 0.01, respectively). The OPLL size at each cervical level significantly correlated with MFD of the same or 1–3 adjacent levels. ΔCobb angle was negatively correlated with the average Goutallier grade (r = -0.31, p < 0.01) and average OPLL occupying diameter and area ratios (r = -0.31, p < 0.01; r = -0.35, p < 0.01, respectively). Patients with continuous OPLL exhibited worse MFD than those with segmental OPLL (p < 0.01).Conclusion: OPLL size is clinically correlated with MFD and cervical ROM. OPLL at one spinal level affects MFD at the same and 1–3 adjacent spinal levels. The worsening severity of MFD is associated with the longitudinal continuity of OPLL.

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“…Recently, various ncRNAs have been found to be physically associated with chromatin, which are defined as chromatin-associated ncRNAs. As one of the ncRNAs, lncRNAs, are widely involved in epigenetic regulation of skeletal muscle development [ 4 , 7 – 9 ]. Currently, only a fraction of lncRNAs have been well authenticated; however, they appear to interact with chromatin through diverse roles [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

MYH1G-AS is a chromatin-associated lncRNA that regulates skeletal muscle development in chicken

Cai,

Ma,

Yuan

et al. 2024

Cell Mol Biol Lett

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Background Skeletal muscle development is pivotal for animal growth and health. Recently, long noncoding RNAs (lncRNAs) were found to interact with chromatin through diverse roles. However, little is known about how lncRNAs act as chromatin-associated RNAs to regulate skeletal muscle development. Here, we aim to investigate the regulation of chromatin-associated RNA (MYH1G-AS) during skeletal muscle development. Methods We provided comprehensive insight into the RNA profile and chromatin accessibility of different myofibers, combining RNA sequencing (RNA-seq) with an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). The dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were used to analyze the transcriptional regulation mechanism of MYH1G-AS. ALKBH5-mediated MYH1G-AS N6-methyladenosine (m6A) demethylation was assessed by a single-base elongation and ligation-based qPCR amplification method (SELECT) assay. Functions of MYH1G-AS were investigated through a primary myoblast and lentivirus/cholesterol-modified antisense oligonucleotide (ASO)-mediated animal model. To validate the interaction of MYH1G-AS with fibroblast growth factor 18 (FGF18) protein, RNA pull down and an RNA immunoprecipitation (RIP) assay were performed. Specifically, the interaction between FGF18 and SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (SMARCA5) protein was analyzed by coimmunoprecipitation (Co-IP) and a yeast two-hybrid assay. Results A total of 45 differentially expressed (DE) lncRNAs, with DE ATAC-seq peaks in their promoter region, were classified as open chromatin-associated lncRNAs. A skeletal muscle-specific lncRNA (MSTRG.15576.9; MYH1G-AS), which is one of the open chromatin-associated lncRNA, was identified. MYH1G-AS transcription is coordinately regulated by transcription factors (TF) SMAD3 and SP2. Moreover, SP2 represses ALKBH5 transcription to weaken ALKBH5-mediated m6A demethylation of MYH1G-AS, thus destroying MYH1G-AS RNA stability. MYH1G-AS accelerates myoblast proliferation but restrains myoblast differentiation. Moreover, MYH1G-AS drives a switch from slow-twitch to fast-twitch fibers and causes muscle atrophy. Mechanistically, MYH1G-AS inhibits FGF18 protein stabilization to reduce the interaction of FGF18 to SMARCA5, thus repressing chromatin accessibility of the SMAD4 promoter to activate the SMAD4-dependent pathway. Conclusions Our results reveal a new pattern of the regulation of lncRNA expression at diverse levels and help expound the regulation of m6A methylation on chromatin status. Graphical Abstract

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The Functional Role of Long Non-Coding RNA in Myogenesis and Skeletal Muscle Atrophy

Cited by 12 publications

References 154 publications

lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p

lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p

Correlation Between the Severity of Multifidus Fatty Degeneration and the Size of Ossification of Posterior Longitudinal Ligament at Each Spinal Level

MYH1G-AS is a chromatin-associated lncRNA that regulates skeletal muscle development in chicken

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