The functional response of alginate-gelatin-nanocrystalline cellulose injectable hydrogels toward delivery of cells and bioactive molecules

Wang, K.; Nune, K. C.; Misra, R.D.K.

doi:10.1016/j.actbio.2016.03.016

Cited by 183 publications

(107 citation statements)

References 26 publications

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“…Soft composites consisting of κ-carrageenan gel reinforced with two types of nanocellulose (MFC and NCC) for many applications including drug release system was developed [65]. In other studies, hybrid injectable hydrogels comprising of alginate and nanocellulose were synthesized for delivery of cells and bioactive molecules [66,67]. Park et al [68] developed bacterial nanocellulose alginate hydrogel for cell encapsulation.…”

Section: Seaweed Based Nanocomposite Materialsmentioning

confidence: 98%

Seaweed based sustainable films and composites for food and pharmaceutical applications: A review

Khalil

Saurabh

Tye

et al. 2017

Renewable and Sustainable Energy Reviews

153

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Section: Seaweed Based Nanocomposite Materialsmentioning

confidence: 98%

Seaweed based sustainable films and composites for food and pharmaceutical applications: A review

Khalil

Saurabh

Tye

et al. 2017

Renewable and Sustainable Energy Reviews

153

View full text Add to dashboard Cite

“…Hence, recent studies propose blending polymers to extend the biodegradation period. Seaweed blends with cellulose have significantly prolonged the shelf life of films due to the homogeneous dispersion and hydrophobicity of cellulose [98,99]. 4.3.4.…”

Section: Biodegradability Of Seaweed and Cellulosementioning

confidence: 99%

“…Application: food packaging Alginate/NCC [99] The hybrid hydrogels exhibited desired mechanical properties and improve resistance to biodegradation. Application: hydrogel film Carrageenan/NFC/crosslinking with gelatin [100] Addition of CNF improves the tensile strength, water vapor transmission rate & oxygen transmission rate of film.…”

Section: Preparation Of Seaweed/cellulose Blendsmentioning

confidence: 99%

Biodegradable polymer films from seaweed polysaccharides: A review on cellulose as a reinforcement material

Khalil¹,

Tye²,

Saurabh³

et al. 2017

Express Polym. Lett.

181

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“…After mixing for 15 min, 2% gelatin (20 mg/ml of nHA suspension) and 2% alginate (20 mg/ml of nHA suspension) were added and stirred overnight at 100 rpm and 40 °C. The 1:1 ratio of gelatin and alginate was based on a study, previously carried out in our group (Wang, Nune, & Misra, ). Furthermore, 250‐mg EDC was added and stirred at 40 °C for 1 hr, followed by mixing of 150‐mg NHS for 10 min to make the prehydrogel.…”

Section: Methodsmentioning

confidence: 99%

Design and biological functionality of a novel hybrid Ti‐6 A l‐4 V /hydrogel system for reconstruction of bone defects

Kumar

Nune

Misra

2017

J Tissue Eng Regen Med

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We have designed a unique injectable bioactive hydrogel comprising of alginate, gelatin, and nanocrystalline hydroxyapatite and loaded with osteoblasts, with the ability to infiltrate into three-dimensional Ti-6Al-4V scaffolds with interconnected porous architecture, fabricated by electron beam melting. A two-step crosslinking process using the EDC/NHS and CaCl was adopted and found to be effective in the fabrication of cell-loaded hydrogel/Ti-6Al-4V scaffold system. This hybrid Ti-6Al-4V scaffold/hydrogel system was designed for the reconstruction of bone defects, which are difficult to heal in the absence of suitable support materials. The hybrid Ti-6Al-4V/hydrogel system favourably modulated the biological functions, namely, adhesion, proliferation, cell-to-cell, and cell-material communication because of the presence of extracellular matrix-like hydrogel in the interconnected porous structure of 3D printed Ti-6Al-4V scaffold. The hydrogel was cytocompatible, which was proven through live/dead assay, the expression level of prominent proteins for cell adhesion and cytoskeleton, including 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Furthermore, the high bone formation ability of the hydrogel was confirmed using alkaline phosphatase assay. A high equilibrium water content (~97%) in the hydrogel enables the delivery of cells and bioactive molecules, necessary for bone tissue growth. Although not studied, the presence of hydrogel in the pores of the scaffold can provide the space for the cell migration as well as vascularization through it, required for the effective exchange of nutrients. In conclusion, we underscore that the 3D-printed Ti-6Al-4V scaffold-loaded with bioactive hydrogel to treat the bone defects significantly impacted cellular functions and cell-material interaction.

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The functional response of alginate-gelatin-nanocrystalline cellulose injectable hydrogels toward delivery of cells and bioactive molecules

Cited by 183 publications

References 26 publications

Seaweed based sustainable films and composites for food and pharmaceutical applications: A review

Seaweed based sustainable films and composites for food and pharmaceutical applications: A review

Biodegradable polymer films from seaweed polysaccharides: A review on cellulose as a reinforcement material

Design and biological functionality of a novel hybrid Ti‐6 A l‐4 V /hydrogel system for reconstruction of bone defects

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