The functional proteomics toolbox: methods and applications

Hunter, Thomas C.; Andon, Nancy L.; Koller, Antonius; Yates, John R.; Haynes, Paul A.

doi:10.1016/s1570-0232(02)00570-6

Cited by 61 publications

(38 citation statements)

References 176 publications

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“…environment (Taraszka et al, 2005). The emerging field of developmental proteomics, in which large mixtures of proteins are characterised in a single experimental sequence, may allow for the assessment of variability or similarity in an individual at the level of the proteome (Hunter et al, 2002). The developmental proteomics of rice is perhaps the least studied, but its importance was realised when the proteome of rice at 5 days and the third and fourth leaf stages were analysed (Jung et al 2008;Chen et al, 2009).…”

Section: Exploring the Variability Of The Developmental Stage Specifimentioning

confidence: 99%

Comparative Analyses of Extracellular Matrix Proteome: An Under-Explored Area in Plant Research

Narula¹,

Elagamey²,

Datta³

et al. 2012

Crop Plant

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Section: Exploring the Variability Of The Developmental Stage Specifimentioning

confidence: 99%

Comparative Analyses of Extracellular Matrix Proteome: An Under-Explored Area in Plant Research

Narula¹,

Elagamey²,

Datta³

et al. 2012

Crop Plant

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“…It is known that a polysulfoethyl aspartamide stationary phase enables the use of smaller amounts of organic modifier, compared to the other SCX sorbents [10], and is hence preferred as a first dimension SCX stationary phase in a 2-D separation system for peptides. In addition, polysulfoethyl aspartamide has (twice as) high loading capacity, and an excellent selectivity for peptides [11] compared to typical silica-based SCX. or by autohydrolysis.…”

Section: Column Bleedmentioning

confidence: 99%

Determination and removal of impurities in 2‐D LC‐MS of peptides

Mihailova¹,

Lundanes

Greibrokk

2006

J of Separation Science

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Problems occurring during operation of a 2-D LC-MS system for separation and identification of neuropeptides, such as contamination of the used salts and column bleed, are described. When using polysulfoethyl aspartamide, which is widely used as a strong cation exchange stationary phase in the first dimension, interfering peaks were observed in the second-dimension reversed-phase chromatograms. The observed peaks, found to be caused by column bleeding, had abundance above the threshold value and influenced the quality of the analyses. The origin of the peaks was verified and appropriate measures are proposed. Additionally, peaks caused by polyethylene glycols (PEGs), covering approximately 5 min of feasible chromatographic time in every fraction, were observed. The commercial ammonium formate salts used to prepare the first-dimension mobile phase were found to contain PEG impurities, and in subsequent work the salt solutions were prepared from formic acid and ammonia to avoid any additional contaminations.

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“…This is a huge task, partly due to the complex variety of modification forms of most proteins possess and constant changes in proteome with time (2). Comparative proteomics aims to characterise the biological mechanisms that form the link between observable phenotypes and genotypes, thereby making moment by moment snap shots of cellular responses at the protein level (3).…”

Section: Introductionmentioning

confidence: 99%