Hereditary disorders of primary haemostasis, characterized by mucocutaneous bleeding (MCB), are highly prevalent in children. Few cases are clearly monogenic but the overwhelming majority are classified as mild bleeding disorders, with wide clinical and laboratory heterogeneity suggestive of complex polygenic diseases. In this framework, and by homology with venous thrombosis, some functional polymorphisms affecting the haemostatic system should be considered. We evaluated the role of 18 common haemostatic polymorphisms on the occurrence and severity of MCB in a casecontrol study including 269 patients and 286 matched controls consecutively recruited. FV Leiden was associated with milder bleeding severity, assessed by a standardized bleeding score (p=0.013). Multivariate analysis revealed that three additional polymorphisms protected against MCB (F13 Leu34, OR=0.66, 95%CI:0.47-0.94, p=0.024; VKORC1 1173T, OR=0.59, 95%CI:0.40-0.87¸ p=0.009; and non-O blood group alleles, OR=0.59, 95%CI:0.41-0.86¸ p=0.006). When combined, these polymorphisms showed an additive protection (OR=0.24; 95% CI:0.11-0.52), supporting the polygenic nature of MCB. Our data suggest that some common polymorphisms affecting haemostasis-related genes could protect from bleeding.Response to Reviewers: see attachment supporting the polygenic nature of MCB. Our data suggest that some common polymorphisms affecting haemostasis-related genes could protect from bleeding.
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